EMAGE mouse gene expression spatial database

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EMAGE:7180

Hpca hippocalcin ( MGI:1336200)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002625. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002625_01	euxassay_002625_02	euxassay_002625_03	euxassay_002625_04

euxassay_002625_05	euxassay_002625_06	euxassay_002625_07	euxassay_002625_08	euxassay_002625_09

euxassay_002625_10	euxassay_002625_11	euxassay_002625_12	euxassay_002625_13	euxassay_002625_14

euxassay_002625_15	euxassay_002625_16	euxassay_002625_17	euxassay_002625_18	euxassay_002625_19

euxassay_002625_20	euxassay_002625_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7180_wholemount_strong.wlz

7180_wholemount_moderate.wlz

7180_wholemount_weak.wlz

7180_wholemount_possible.wlz

7180_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7180_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02

telencephalon marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 weak expression: see section 07 15 16 17 18 19 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 06 07 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 18 weak expression: see section 03 04 05 06 07 14 15 16 17 19

dorsal root ganglion

weak

regional

weak expression: see section 07 08 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5276
Entity Detected:	Hpca, hippocalcin ( MGI:1336200)
Sequence:	sense strand is shown >T5276 GACTTGGCTCTTCTGCCATGGGCAAGCAGAACAGCAAGCTGCGGCCAGAGATGCTGCAGGACCTGCGAGA GAACACGGAGTTCTCTGAGCTGGAGCTACAGGAGTGGTACAAGGGCTTCCTGAAGGACTGCCCCACTGGC ATCCTCAACGTGGATGAGTTCAAGAAGATCTACGCCAACTTCTTCCCCTATGGCGACGCCTCCAAGTTTG CCGAGCACGTCTTCCGCACCTTTGACACCAACAGCGACGGCACCATCGACTTCCGGGAGTTCATCATTGC TCTGAGCGTGACCTCGCGTGGCCGCCTGGAGCAGAAGCTCATGTGGGCCTTCAGCATGTATGACCTGGAT GGCAATGGCTACATCAGCCGGGAGGAGATGCTAGAAATTGTGCAGGCCATTTACAAGATGGTTTCGTCCG TGATGAAAATGCCCGAGGATGAGTCGACCCCGGAAAAGAGGACTGAGAAAATCTTCCGCCAAATGGACAC AAACAATGACGGCAAGCTGTCGCTGGAGGAGTTCATCCGCGGAGCCAAAAGCGACCCATCCATCGTGCGC CTGCTGCAGTGTGATCCCAGCAGCGCCTCCCAGTTCTGAGCTGAGAGGAGCCAGCTCGCCTCCCTGCCTT CACCGGCCCTCCGGCTCTCAGCTTCCTCTCCCTCGTGTCTATCCAGGCTGGGGGCCAGACTGGGGATCCC TTTCTGGGGTCTGCACTGTGTGTGTGTGTGGGCCTCTGGAGAAGTGAAACCTGCAAGGAAGGGGCTAAGC AAGACAGCTGTTAGTCTCCACGGCCCAAGAGGCAGAATCTCCCTTAGTGGGACCGAGATAGCCCTCCGGT CAGGTTCTGTATTCTTTAGTGAGACAGCTGGATCATCTTACACCCACCCACAGTCAGGAGGATCTTCCAC AACACGGGGGCAGGGGGGCGGGTAGGTCCCCTTTGGGTGGTCCTCAGCCTGGAGGGAGGTGGGGAGTCAT GCCAAGGTGAAGAGTCTTTTTGTTTGGTGGGGAGCCACGGATGATTTCTGGGCTGTGGGGCTTTGAAGAA GTTCCCTGATCTACTTCCCTGCAGAAGAACACTTCTTGTCCCAGCTCTTGCTGGCTGGAGGCCATGGTAA AACAAAACAAAACTCCCAGGATTCCAACTGGGGGTCCCCAAACTCAACCTGCCTCTTTGCACTTAGATAA CCACAGATTTACCCCGTCTGTACTCTGTATTTCACCTCTCTCTCTCGTGCACTCACGCAAACACACACAC ACACACACACACACACACACACACACACACACACACAGAGGCCCACAGACAGATGGGCAGAGCCCCTCCA CAGCATTCTGTGTGGGAAGGAAGCGCAGGCCCCCGGCCAAGCCCCATGTCCCTCTGCATGCAGCCACACG GAGCATCTTGGTTCTTTTTAATAACTTCAAAATAAAGTCTTGTTTCAGTGCAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5360894 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5360894 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7181 same embryo
	EMAGE:7183 same embryo
	EMAGE:7184 same embryo
	EMAGE:7179 same embryo
	EMAGE:7182 same embryo
	EurExpress:euxassay_002625 same experiment
	MGI:4825441 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source