EMAGE mouse gene expression spatial database

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EMAGE:7185

2810403A07Rik RIKEN cDNA 2810403A07 gene ( MGI:1921450)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002710. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002710_01	euxassay_002710_02	euxassay_002710_03	euxassay_002710_04

euxassay_002710_05	euxassay_002710_06	euxassay_002710_07	euxassay_002710_08	euxassay_002710_09

euxassay_002710_10	euxassay_002710_11	euxassay_002710_12	euxassay_002710_13	euxassay_002710_14

euxassay_002710_15	euxassay_002710_16	euxassay_002710_17	euxassay_002710_18	euxassay_002710_19

euxassay_002710_20	euxassay_002710_21	euxassay_002710_22	euxassay_002710_23	euxassay_002710_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7185_wholemount_strong.wlz

7185_wholemount_moderate.wlz

7185_wholemount_weak.wlz

7185_wholemount_possible.wlz

7185_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7185_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5901
Entity Detected:	2810403A07Rik, RIKEN cDNA 2810403A07 gene ( MGI:1921450)
Sequence:	sense strand is shown >T5901 GTAGTTGGCGGGTGGTTGACTGGAGCAGGCCGCCATGTCCGCAGGGAGCGCGACACATCCTGCCGCTGGC GGGCGCCGCAGCAAATGGGATCAGCCAGCGCCAGCTCCGCTTCTCTTCCTGCCGCCAACGGCCCCAGGTG GAGAAGTCGCTGGCAGCGGGGCGAGCCCCGGGGGCGCCACCACCGCCGCTCCTTCAGGAGCCTTGGATGC CGCCGCTGCCGTGGCTGCCAAGATCAATGCTATGCTCATGGCCAAAGGGAAGCTGAAACCATCTCAGAAT GCGGCCGAGAAGCTTCAGGCCCCTGGCAAAAGCCTAACTAGCAATAAAAGCAAAGATGACCTGGTGGTGG CTGAAGTAGAAATCAATGACGTGCCTCTGACATGCAGAAACCTGCTGACACGAGGACAGACACAAGATGA GATCAGTCGGCTTAGTGGGGCTGCTGTGTCAACTCGGGGACGGTTCATGACGACTGAGGAGAAGGCCAAA GTGGGACCTGGAGACCGTCCATTGTATCTTCATGTTCAGGGACAGACACGGGAATTAGTAGACAGAGCTG TAAACCGAATCAAAGAAATAATCACCAATGGAGTGGTGAAGGCTGCCACAGGGACAAGTCCAACTTTCAA TGGTGCAACAGTCACTGTCTATCACCAGCCAGCTCCCATTGCTCAGTTATCACCTGCTATTAACCAGAAG CCTTCCTTCCAGTCCGGAATGCATTATGTTCAAGATAAACTATTTGTGGGTCTAGAACATGCGGTGCCCA CTTTTAATGTTAAAGAGAAAGTAGAAGGTCCAGGCTGCTCTTATTTGCAACACATTCAAATTGAAACTGG TGCCAAAGTCTTTCTACGAGGGAAAGGCTCAGGCTGCATTGAGCCAGCGTCAGGCCGAGAGGCTTTTGAA CCCATGTATATTTATATCAGTCATCCCAAACCTGAAGGCCTGGCTGCTGCCAAGAAGCTCTGCGAGAATC TCTTACAAACAGTTCATGCTGAGTATTCTAGATTTGTGAATCAAATTAATACTGCTGTACCATTACCAGG CTATACACAACCCTCTGCTATAAGTAGTATCCCTCCTCAACCACCATATTATCCATCCAATGGCTATCAG TCTGGTTACCCTGTTGTTCCCCCTCCTCAGCAGCCAGTTCAGCCTCCCTACGGAGTACCAAGCATAGTGC CACCAGCTGTTTCATTGGCACCTGGAGTCTTGCCAGCATTACCTACTGGGGTCCCACCTGTGCCAACACA GTATCCGATAACACAAGTGCAACCTCCAGCCAGCACTGGACAGAGCCCAATAAGTGCTCCTTTTATTCCT GCTGCTCCTGTCAAAACTGCCTTGCCTACTGGCCCCCAGCCCCAGCCCCAACTCCCAGCTCAGCCACAGT CACAGAAGAGGCGATTCACAGAGGAGCTGCCTGATGAACGGGACTCTGGACTGCTTGGATACCAGCATGG ACCCATTCATATGACTAATTTAGGTACAGGCTTCTCCAGTCAGAATGAGATAGAAGGCGCAGGATCGAAG CCGGCAAGTTCCTCAGGCAAAGAGCGAGAGAGGGACAGGCAGTTGATGCCTCCACCAGCCTTTCCAGTGA CTGGAATAAAAACTGAGTCTGATGAAAGGAATGGGTCTGGGGCCTTAGCCGGAAGCCATGATTATCCAGC CAAGAAGATGAAAACGGCAGAGAAGGGATTTGGCTTAGTAGCTTACGCTGCAGACTCCTCTGATGAAGAG GAAGAACATGGAGGTCATAAAAATGCAAGTAGCTTTCCACAGGGCTGGAGTTTGGGCTATCAGTATCCTT CATCACAGCCACGAGCTAAGCAGCAGATGCCATTTTGGATGGCCCCATAGAAAATAGTGGACAGAGCTTT GACCCTCAGTGACTCTTCTTAGCAATAATGCATGCACTTGATTAAACAAGACTCTGGGACCTGTTCTGGG GGCCATCTTGGATCTTCGAAGATGTTAGGGACACAGTGTCCCCTGTCATAAAGGGACGCCATTCTTTTTA AAACCCTATTTCTGCAGTGGCATAACATCCGTTAAAATTCACTTAGAATCACAAATTTGCATCAAGCTTT TTAACAGTTGATGAAATGTGCTTGTCCAGTGAGATACCAGGACAGAGTCATTGCTACATATGTTTGACCT GATCTAGCCTTTTCCAAATGAATAGCCTCAACTCTGTTGTAAAGAAAGTTTTATTTGTATTTTACTACTA TTGGGTCAATTTTGATAACTGGTTACAAACAAAAAGCCTTACTATTTATTAGTGGGAAATGGTTTTAAGA CCCTTCCTTCCATACTTACATGTAATTCTAAGAGCTCCTCATCCCTCTTTCCATTTGGAGTCTTTGTTTG GAAAATGCTGTTTTACTAATTTAGAATGGTCAAATACAACTGGATCCTGAACAGGAAAATGCAGTTATTT TTGCATTCTTTCTTTACTGGGATGATCCAAAGCTGGCACCTTTAGGCACACTTGTTTCAGATAGCCATTG CTGTTGGCACTGCCCTTCTAAGCTCCCGGTTTCCAACTGGGTCAAAGAAAACGCGAAACACTCATCTGTC TCTCTCAGAACTGGCTGCAGCGAGAAGAGGGATGTGGTGCCACCGACAGGACGGTGACTGACTGGAATTG CAACTTGTCCTTTTTTCTTGGCATTGCGGGTTTTGCCAAAAGAACTTTTTTGTATCAAATATTCATGTGT GGAAGTGAAGGAATTGGTGGTGAGCCAGGAGCGTCTGAGATACTGTCATTTTTGCACATTTTTGAATACT TTGCGGGCTGGCTTTGTACAAACTTATTCTCTGGTTTCTTGTATGTTGTAAATAATTAGACCATAATTTC ATTATAAATAAATGTATAAATATTCTGCTTGTCATTTCTCTTTTTCTTCCCTAGCCTGTTCATACACTAA TATCAATAAACTCTTCAATTATTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3158104 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3158104 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002710 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source