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EMAGE:719

Nog noggin ( MGI:104327)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:719 EMAGE:719
Fig 1I Goldman et al, 2000 [PMID:10769235] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 1J Goldman et al, 2000 [PMID:10769235] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The section in Image J was taken through a whole mount stained sample similar to I. The section contains the VER (ventral ectodermal ridge) at the bottom.
Expression Pattern Description
Spatial Annotation:
EMAGE:719Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
719_wholemount_strong_3D_1.wlz
719_wholemount_moderate_3D_1.wlz
719_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:719_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
surface ectoderm
not detected not detected
regionalThere is no expression in the ventral ectodermal ridge
neural tube
detected detected
regionalExpression is dorsal
notochord
detected detected
regionalExpression is noted in the tail
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1355230
Entity Detected:Nog, noggin ( MGI:104327)
Notes:The probe used in this study by Goldman et al, 2000 [PMID:10769235] is described as follows: "Digoxigenin-labeled riboprobes were prepared from plasmids described in references cited for each gene". The reference given for Nog in the Results section is Zimmerman et al, 1996 [PMID:8752214] who refer to Xenopus noggin and no in situ probes.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ARC/S or CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
General Information
Authors:Goldman et al, 2000 [PMID:10769235] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10769235] Goldman DC, Martin GR, Tam PP 2000 Fate and function of the ventral ectodermal ridge during mouse tail development. Development (127):2113-23
 [ doi:10.1016/S0092-8674(00)80133-6] [ PMID:8752214] Zimmerman LB, De Jesus-Escobar JM, Harland RM 1996 The Spemann organizer signal noggin binds and inactivates bone morphogenetic protein 4. Cell (86):599-606
Links:MGI:1355379 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI