EMAGE mouse gene expression spatial database

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EMAGE:7198

Slc29a1 solute carrier family 29 (nucleoside transporters), member 1 ( MGI:1927073)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002695. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002695_01	euxassay_002695_02	euxassay_002695_03	euxassay_002695_04

euxassay_002695_05	euxassay_002695_06	euxassay_002695_07	euxassay_002695_08	euxassay_002695_09

euxassay_002695_10	euxassay_002695_11	euxassay_002695_12	euxassay_002695_13	euxassay_002695_14

euxassay_002695_15	euxassay_002695_17	euxassay_002695_18	euxassay_002695_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7198_wholemount_strong.wlz

7198_wholemount_moderate.wlz

7198_wholemount_weak.wlz

7198_wholemount_possible.wlz

7198_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7198_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 weak expression: see section 09 10 12

liver

strong

regional

strong expression: see section 01

liver lobe

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5852
Entity Detected:	Slc29a1, solute carrier family 29 (nucleoside transporters), member 1 ( MGI:1927073)
Sequence:	sense strand is shown >T5852 GAGAAAGGCCCAGGGCAGTGTGGAAGCAGAGCCAGACAGGGCTCGATATTCCTTTACCCCCAGCAAGAGC CAGAGGGAGGGAGCTGCCAGCCAGGCACAGCCGAGAACACTGGAGCCATGACAACCAGTCACCAGCCTCA GGACAGGTATAAGGCAGTATGGCTTATCTTCTTTGTGCTGGGCCTGGGGACACTGCTCCCCTGGAATTTT TTTATGACCGCAACCAAGTATTTCACAAACCGCCTGGACGTGTCCCAGAATGTGTCCTCGGACACTGATC AATCATGCGAAAGCACCAAGGCCTTGGCTGACCCCACAGTGGCCTTGCCAGCCCGGAGTTCTCTCAGTGC CATCTTCAACAATGTCATGACCCTGTGTGCCATGCTGCCCTTGCTGGTCTTCACCTGCCTCAACTCGTTT CTGCATCAGCGGATCTCTCAATCTGTTCGGATCTTGGGCAGCCTGCTGGCAATCCTGCTGGTATTCCTTG TCACTGCCGCCCTGGTGAAGGTGGAGATGGATGCTCTGATCTTCTTTGTCATCACCATGATCAAGATTGT GCTCATCAATTCATTTGGTGCCATTTTGCAAGCCAGCCTTTTTGGTCTGGCAGGTGTCCTGCCAGCCAAC TACACAGCCCCCATCATGAGTGGCCAGGGCCTGGCTGGCTTCTTCACCTCTGTCGCCATGATCTGTGCCA TTGCCAGTGGTTCTGAGCTGTCAGAAAGCGCCTTTGGCTACTTCATCACAGCCTGTGCAGTTGTCATTTT GGCCATCCTGTGCTACCTGGCTCTGCCTCGGACGGAATTCTATCGCCATTACCTGCAGCTCAACCTTGCG GGGCCTGCAGAGCAGGAGACCAAGTTGGATCTCATAAGAGAGGAGCCAAAAGGAAGAAGAGAGGAATCTG GGGTGCCAGGCCCCAACTCTCCACCCACCAACAGAAACCAGTCTATCAAAGCCATACTTAAGAGTATCTG TGTCCCGGCTCTGTCTGTCTGCTTCATCTTCACGGTTACCATTGGGTTGTTCCCTGCTGTGACTGCTGAG GTGGAATCCAGCATCGCAGGCACAAGTCCCTGGAAAAGCTACTTCATTCCCGTGGCCTGTTTCTTGAATT TCAATGTCTTTGACTGGCTAGGCCGGAGCCTCACTGCTGTCTGCATGTGGCCTGGCCAGGATAGCCGCTG GCTGCCGGTTTTGGTCGCCTCGAGGATTGTGTTTATTCCCCTGCTGATGCTCTGCAACGTGAAGGCTCGC CACTGCGGCGCGCAGCGGCACCACTTCGTCTTTAAGCATGACGCCTGGTTCATCGCCTTCATGGCTGCCT TTGCCTTCTCCAATGGCTACCTCGCCAGCCTCTGCATGTGCTTCGGGCCCAAGAAAGTCAAACCAGCTGA GGCGGAGACAGCAGGAAACATCATGTCCTTCTTTCTGTGTCTGGGCCTGGCTCTGGGAGCTGTGTTGTCC TTCTTGTTAAGGGCACTTGTGTGACCCTGTGGGGACAGAAGAACTACACTGCCTGCTTCCTGCTCACTTC CTTCCCTGCCAGGGACGAGCAGGGGTCGAGAGGGGCTGTTCTTCTAGCTGACTTCTGCTTTCCTCTGGAC TGTGCTTCGCCCAGCTGTCCAGGAGCCAGCGATGGCCTGCGGGTGGACTTGGAATTCAGGGTCAGAATGG CAAGGGCTCAATGGCCTCTGACTGACAGCTCCGACTGATGCCCGCTTACTCCAAGCACAAGAGACTCCAG GGCCAAGAGAGATCTGTCCGCCTGCCTATCACAGGATAGGGCGGAGGCGGATGGCTGATTGGTGTCGTGT GACCTGATGTCCCTCCCCTTGCCCTTCTTCCTTCTGTGCCTGTTCCATGTCCCCAGCCCTTGTCATTTTA CTGCCTTTTTTATACTGACAGAAACCAGGTGCCTTCAGAGGCCATCTGATTAAATAAACATTTTTTTTCT CCATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3155188 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3155188 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7199 same embryo
	EMAGE:7197 same embryo
	EMAGE:7196 same embryo
	EMAGE:7195 same embryo
	EMAGE:7194 same embryo
	EurExpress:euxassay_002695 same experiment
	MGI:4828176 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source