EMAGE mouse gene expression spatial database

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EMAGE:7221

Baiap2 brain-specific angiogenesis inhibitor 1-associated protein 2 ( MGI:2137336)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002713. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002713_01	euxassay_002713_02	euxassay_002713_03	euxassay_002713_04

euxassay_002713_05	euxassay_002713_06	euxassay_002713_07	euxassay_002713_08	euxassay_002713_09

euxassay_002713_10	euxassay_002713_11	euxassay_002713_12	euxassay_002713_13	euxassay_002713_14

euxassay_002713_15	euxassay_002713_16	euxassay_002713_17	euxassay_002713_18	euxassay_002713_19

euxassay_002713_20	euxassay_002713_21	euxassay_002713_22	euxassay_002713_23	euxassay_002713_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7221_wholemount_strong.wlz

7221_wholemount_moderate.wlz

7221_wholemount_weak.wlz

7221_wholemount_possible.wlz

7221_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7221_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

strong

homogeneous

strong expression: see section 11 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5902
Entity Detected:	Baiap2, brain-specific angiogenesis inhibitor 1-associated protein 2 ( MGI:2137336)
Sequence:	sense strand is shown >T5902 GTCTGCGCGCCTTTTCCTGTTGCTGCAGTTGTCGCTTTCCTCACCGCCACCCGTGCCCCTGCTCTGGTCT GTGGTGTAGCCGGGACCCAGGACCATGTCGCTTTCACGCTCGGAGGAGATGCACCGGCTCACGGAAAATG TCTACAAGACCATCATGGAGCAGTTCAACCCCAGCCTCCGCAACTTCATCGCCATGGGCAAGAACTATGA GAAAGCACTGGCAGGTGTCACCTTCGCTGCCAAAGGCTATTTCGATGCTCTGGTAAAGATGGGGGAGCTG GCCAGCGAGAGCCAGGGCTCTAAGGAACTTGGGGACGTCCTCTTCCAGATGGCTGAGGTGCACCGGCAGA TCCAGAACCAGTTGGAAGAGACGCTGAAGTCTTTTCACAATGAGCTGCTCACGCAGCTGGAGCAGAAAGT AGAACTGGACTCCAGGTATCTAAGTGCTGCACTGAAGAAATACCAAACGGAACAGAGGAGCAAAGGGGAC GCCCTGGACAAGTGTCAGGCTGAGCTGAAGAAGCTCCGCAAGAAGAGCCAAGGGAGTAAGAACCCTCAGA AGTACTCAGACAAGGAGCTGCAGTACATCGATGCCATCAGCAATAAGCAGGGCGAGCTGGAGAACTACGT GTCTGACGGCTACAAGACAGCACTCACTGAGGAGCGCAGGAGGTTCTGCTTCCTGGTGGAAAAGCAGTGC GCTGTGGCCAAGAACTCTGCTGCTTACCATTCCAAGGGCAAGGAGTTGCTGGCCCAGAAGCTGCCTCTGT GGCAGCAAGCCTGTGCCGACCCCAACAAGATCCCAGACCGTGCTGTCCAGCTGATGCAACAGATGGCCAA TAGCAATGGCTCCATCCTTCCAAGTGCCCTGTCAGCTTCCAAGTCCAACCTGGTCATCTCAGATCCCATT CCTGGAGCCAAGCCCTTGCCAGTGCCACCTGAGCTGGCACCATTTGTGGGGCGGATGTCTGCTCAGGAGA ATGTACCTGTCATGAATGGCGTCGCAGGCCCGGACAGCGAGGACTACAACCCCTGGGCTGACCGAAAGGC TGCCCAGCCCAAATCCCTGTCTCCCCCGCAGTCTCAAAGCAAGCTGAGCGACTCGTACTCCAACACACTC CCCGTGCGCAAGAGCGTGACGCCGAAGAACAGCTATGCCACCACTGAGAACAAGACACTGCCACGTTCAA GCTCCATGGCAGCTGGCCTAGAACGTAATGGCCGCATGCGGGTCAAGGCCATTTTCTCCCACGCGGCCGG TGACAATAGCACTCTGCTGAGCTTCAAGGAGGGCGACCTCATCACGCTGCTAGTGCCTGAGGCCCGTGAC GGCTGGCACTATGGGGAGAGTGAGAAGACCAAGATGCGGGGCTGGTTCCCCTTCTCCTACACCCGGGTCC TGGACAGTGACGGAAGTGACAGATTGCATATGAGCCTGCAGCAGGGCAAGAGCAGCAGCACAGGCAACCT CCTAGACAAGGATGACCTGGCCCTCCCCCCTCCTGACTACGGCACGTCCTCCCGGGCATTCCCCACCCAG ACAGCCGGCACATTCAAGCAGAGACCCTACAGCGTGGCCGTTCCTGCCTTCTCTCAGGGTCTGGATGACT ACGGGGCACGGTCTGTGAGCAGGTAAGAGGTTTTAGGCTTCGTTGGGACTGTGGGTGGGTGGCCACAGAC TTGGTTAGAGTAACCTCTCCCTTGGGACCTAGCTTTTCTTACTTTGGGAACCTTGACCCTAAGCCTGCCA CCAGAGCTGGACCCACCCACCCACCCACCCGTCCTGGGCTCCTGTCTGCTCTTAGTACAGTGAGCCCCTC CTCCATCCTGCTGGGTCTTGGTGTCTTGGCCTGCTCTGCTCATCCCGGCAGGCAGGGCAGGGGTGAGGAG GGTTGTCAGCCGCCTCTAGGCCTTCTCTCCCTAGAACCAGGTAGATCCTCACCAAGGGCTGCGGCTGCTG CCTGCCGAGCACAGTCTTATTCTGGGTTCATCATCCTCCCAGAGGCCAGGTAGCCTGGCTGCTGGCTGGC CAGGGCTCTGCCGGGTGGCGGGCGGGCCTGCTGCCCCTGGCCGGCGGTGGGCATGCATCTGGGCAGCTGT CCTGTGCTTTGTTCCACAGTGGCAGTGGCACGCTGGTGTCCACAGTGTGAGGACGCTGACCCCAGGCAGC TGCTCTGAAGAGCTCCCCCCAGTCTCCACCGGTTCCCCTCCTCAGCTCTCGTTGGTTTGTTCTTGGGTTG TTTTCTTTCCCACCCGCTCCTGCCTTTTGGTTGGTGATCCCAGACTCTGAAATCCCCCAGGGTCCATGGT GCTGCTCTCTGCGCCCCTGCGCTCATGCCCCCAGCAGGTGTCCCCAGCCTTGAGCATTAACTGCCGGGTG GGGGCCTGCCGGCTGAGTGGCAAAGCAGCGTTGGCACCTGCTGCCCTAGCCCTGTGGTGTTCACGACGCA CATCCCTTTTCTAAGGCTGGCCACGAGCCAGGGCTAGAGTTTGAAGAAAAAGAAAAAAATCTTTAAAAAA GAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3158004 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3158004 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7222 same embryo
	EMAGE:7224 same embryo
	EMAGE:7223 same embryo
	EMAGE:7220 same embryo
	EurExpress:euxassay_002713 same experiment
	MGI:4823386 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source