EMAGE mouse gene expression spatial database

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EMAGE:7223

Chrnb3 cholinergic receptor, nicotinic, beta polypeptide 3 ( MGI:106212)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004358. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004358_01	euxassay_004358_02	euxassay_004358_03	euxassay_004358_04

euxassay_004358_05	euxassay_004358_06	euxassay_004358_07	euxassay_004358_08	euxassay_004358_09

euxassay_004358_10	euxassay_004358_11	euxassay_004358_12	euxassay_004358_13	euxassay_004358_14

euxassay_004358_15	euxassay_004358_16	euxassay_004358_17	euxassay_004358_18	euxassay_004358_19

euxassay_004358_20	euxassay_004358_21	euxassay_004358_22	euxassay_004358_23	euxassay_004358_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7223_wholemount_strong.wlz

7223_wholemount_moderate.wlz

7223_wholemount_weak.wlz

7223_wholemount_possible.wlz

7223_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7223_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 06 07 08 09 10 12 13 14 15 16

midbrain mantle layer

moderate

single cell

moderate expression: see section 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5238
Entity Detected:	Chrnb3, cholinergic receptor, nicotinic, beta polypeptide 3 ( MGI:106212)
Sequence:	sense strand is shown >T5238 CGTCTCTCTGAAGCAGACGTCATGACAGGCTTCCTACGGGTCTTCTTGGCTCTCAGTGCCACTCTCTCAG GTTCCTGGGTGACTCTCACAGCCACTGCGGGACTCAGCTCAGTGGCTGAACATGAAGACGCACTCCTCAG ACATTTGTTCCAAGGTTATCAGAAATGTGTCCGCCCTGTGTTGAATTCCAGTGACATCATAAAAGTGTAT TTTGGATTAAAGATATCTCAGCTTGTAGATGTGGATGAAAAGAATCAGCTGATGACAACAAACGTGTGGC TGAAGCAGGAATGGACAGACCAAAAATTACGATGGAATCCCGAAGACTATGGTGGAATTAATTCGATAAA GGTTCCATCGGAGTCGCTCTGGCTGCCTGACATAGTTCTCTTTGAAAATGCTGACGGACGTTTTGAGGGC TCTCTCATGACCAAGGCCATTGTGAAATCCAGCGGAACCGTCAGTTGGACTCCTCCCGCCAGCTACAAAA GTTCCTGTACCATGGATGTCACATTTTTCCCGTTCGATAAGCAGAACTGCTCAATGAAGTTCGGATCCTG GACTTACGATGGTACCATGGTCGACCTCATTCTGATCAATGAAAACGTTGACCGGAAAGACTTTTTTGAT AACGGAGAGTGGGAGATACTAAATGCAAAGGGGATGAAGGGCAACAGAAGAGAAGGCTTTTACTCCTATC CGTTTGTTACCTACTCTTTTGTTCTGAGACGCCTGCCCCTGTTTTACACCCTCTTTTTGATAATCCCCTG CCTGGGGCTGTCTTTTCTCACGGTCCTGGTGTTCTACCTACCCTCCGACGAAGGGGAAAAACTCTCCTTA TCCACCTCGGTTTTGGTTTCTTTGACAGTGTTTCTTTTAGTAATCGAAGAAATAATCCCGTCTTCTTCAA AAGTCATCCCTCTCATTGGGGAGTACCTCCTCTTCATTATGATATTTGTCACGCTGTCTATTATTGTCAC GGTTTTTGTAATTAATGTCCACCACAGATCTTCCTCAACGTACCATCCCATGGCCCCCTGGGTGAAGAGG CTGTTTCTGGAAAAACTCCCGAGATGGCTTTGCATGAAGGACCCCAGGGACCGCTTCTCTTTCCCCGATG GAACGGAGAGTAAGGGAACCGTGAGGGGGAAATTTCCAGGGAAAAAGAAGCAGACTCCTACCAGTGATGG AGAAAGAGTTCTGGTCGCTTTCCTGGAGAAGGCCTCTGAGTCCATCAGATACATCTCGAGGCATGTGAAG AAGGAACACTTCATCAGTCAGGTTGTTCAAGACTGGAAATTCGTGGCTCAGGTTCTTGACCGCATCTTCC TCTGGCTCTTTCTGACAGCTTCGGTTTTGGGCTCTGTCCTGATTTTTATTCCAGCCTTGAAGATGTGGAT ACATCGTTTCCATTAGGAACTGCTCTCTGGATCCATGTAGAAGACACACATAGAGAGAATCCCACCTAGA ACTGAGGGCTGCTGGTGTGTTCACAGAAAGGAGTTGCATGCAACAGTTGTGGTGCCCTTGCCTTGTCCTG GGAGCCTTCTGTGACTGGAGGAAACTGAGAAAACGTGGGTTTGAGCTAGTGTGATGGTGGCTGCCATTAG AGATGTATTTGGGCAATTCAGAGAAGTCTCCATGTCATGTTGTTGTGTGGGAGGCTCCGAAATGCTCCAT TTGCCTATCCCTGAACTACTGCATCTGCCCATCCCCCAGACTTCTACATCTGCTCATCCCTAAACTCTTG ATTTTGTCCATCCACTGAACTCCTAGTATCTGCCCAGCCCCTTATCGACTTGTCTGCACACTGGGGCCAT GTGGTACTTCTCTAGCAGTGTGGTTGAACACGTTTTGACAACAGTTTCAGGAATTACACAGGTACAACTC TTGCCACACAGGTCAAAGATGCCACTTGTCAACAAATGTCCCATAAATAGGGTCATTGAAGATGACCTTG AATGACTATGACAGTTCTCTAAGGCAGGGGTTATTGGAAGTTTGTTCACTGCATTGTAAACTTTTCTAAT ACAGGTAGGAACTACACCAGAAAAATAGCCATTAGCTATTAGAACTGCATTCATTAATGCATGTCTTTTT TTTTTTTCTCTTAGAAAATTAAAAGTGTTAAATGTTCTTTGATTATGGTTTTGGCTGGTGTCTTAGGCAG TGTTCTATTACTGTGAAGAGACACTGTAACCATGGCAACAGGTCAGGTGCAGAGGTTTAGTTCACTTTCA TTACGGTGGGAAACATGGTGGCACTCTGGCAGATATGGTGTTGGGAGAGGTAGCCTAGAGTTTTACAGCA AGAGAGAGACTGGTGTGGGCTTTTGAAACCCCAAAGCACACCCCCAGTGACATACTTCCCCCAACAAATC TACACCTCTGGATCCTTCTCAAGGAGTGACAATCCATGATGACTAAGTACTAAAGTATGGGAGCTCATGG GGGCCATTTCTATTCAAGCCACCACAGACAGAACCGGTTTTCACAGAGTTTTCTGCCTCCACCTGTGTCC CCTCTCCCTGTGTCTCCCTCCTTTCCTCATCCCACCCCATGCTCCTCCAGGACAGTCAGGAAGTGTTTAT CAGATTTACTTTGCTGCCGTTGCTTCTGAGTCCTTCCTGGGCTTTGTTAAAGGAGTTTGTTCTTCCACCT CAGTGAAACCCCATGTGCTCTGTTTTATTTGAGACACGGTTCTTCTAAGCAGTTTATCCCTGAGATTTAA AAAACCCTAATCTAAGATTTTATTTTTAGATTACTATAACTGCAACATTCAGTAAGCGCTGCGTTCTCTT ATTCCTACTAGTCTGATTATTTTTTTTCTTCCTAAATAGCTTAAGCTTTTCTTATCTTCAGTCTTAGACA TGTCACACTGTGCCTGAGCAGAGAATGATACCCCCATCCATCTCTATCTCTATTCCCTCCACCCTCCCCT TCTCCTCTCCTCTCTGCCATCTCCTTCTCTCTGCACGCGATGACTAGTGTCTTCAAGCTATGCAGATTCT TTTCTATTTCTCTTACTATCTCTGTTCTGCATTTTCTCTCTTTGTCTCTAAAAATTTCTGATAAAATTTC TTGTCATTGTTGGGCCTGTACTCCTTGTTTATTAACTAACCGGTTGACTCTTCTTTCATACTTTTAAAAT TCTTTAATTAATAGTTTCTTAATTCTTTGACAATTCCATACAACATATTTTGATCCTATTTACCCCAAAT TCCTCCCCTGTAACTTCTCCTAGACCCACTCCCTCACCCTATCCCCCCACATCAGGTCCTTATTATTTAT TTTTAAATGCCCCCCTCCCCTGAGTCCAATTTGTGTTGCCTGCATACTCCGGTGTGGTCCCAGCCTTTGG TCCCAACTCTGCAGCTGGTTCCTCTCTAGCTGTATCAGTCTGTGTGAGCAAGGGAGGGTAGGAGCTCTTG CTGATAGCACAGCGGGAGACTCTGCGCTCAGACTACCAGCATCATGCTTCCAACCCTGGCAAGCAGCCTG CATGAACCCCTCACTGCCCATGGCGGGGTCAGCCCTTGGTCAGACTTCTCTAACTAGTCTTTTGTTTGGA CTCTTCCAATGGTGTTTAAGTGCCTTTGTAGCGGGAATGCTGTAAACCCTGATCTGGAGTTCCCACTGAG TCCAACCACAGCCAGTCGAAGGAGAATCTCTGCTTCAGGTTCTTTAGATCGCCTTGCACAGACTACACGG CTCTCCCACCTCGCTGCCAATAGTGTGCCCACAAGATCCAGCACACGAGATGGATTTTTAAAGAAATGGA TAAACACAGTGGAACAAGAGCTAGGTCACACAGATTGCTGGTCCTAGCTCTGTGGCTAACCAGTGGTGTG AGCAGTGGGCCTTACACACGCTGTCTCCCTGAGGACTTTTTAGCTTGTGGGTCAGCAGAACGAGGTGAGC ATTCGTGACCTTTGAAGTTCTCCTCAGTCCTGTACTCTATCTTTTGGAGGTTTGCTATGGAGCTTCCTCA GTGTTCAACCCCATGGTCCTCACAGTCGCATTGTTCCTTGCTGGGCAGGTAGTGTTGAAGGGTGTGTGTG GTCTGCTCTGTGGCTCCCTGAGGGTAGACATTGTGATAAAAATCAAGGCTTAGCTATGACTTCCTGAATG ATACTGTGGATAAAAGAACGAATAGTTATTATTCCCCCTCCTCCAGGTTGATTTTAATTCATCACATTTG ATGTGTGATTACACTAGGAAAAGTGCAGTATCAATGTTAGAAAAAAATGTTTGGATCTGAGCTTAGCTTT ACATTTGGAAAAATACCAGCACGAACCATTTCCCACAACAAATATTTTCTGTTGAATCAAATAAAGAAAA GATTAAGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5357762 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5357762 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7222 same embryo
	EMAGE:7221 same embryo
	EMAGE:7224 same embryo
	EMAGE:7220 same embryo
	EurExpress:euxassay_004358 same experiment
	MGI:4823862 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source