EMAGE mouse gene expression spatial database

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EMAGE:7224

Dhcr7 7-dehydrocholesterol reductase ( MGI:1298378)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002715. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002715_01	euxassay_002715_02	euxassay_002715_03	euxassay_002715_04

euxassay_002715_05	euxassay_002715_06	euxassay_002715_07	euxassay_002715_08	euxassay_002715_09

euxassay_002715_10	euxassay_002715_11	euxassay_002715_12	euxassay_002715_13	euxassay_002715_14

euxassay_002715_15	euxassay_002715_16	euxassay_002715_17	euxassay_002715_18	euxassay_002715_19

euxassay_002715_20	euxassay_002715_21	euxassay_002715_22	euxassay_002715_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7224_wholemount_strong.wlz

7224_wholemount_moderate.wlz

7224_wholemount_weak.wlz

7224_wholemount_possible.wlz

7224_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7224_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 07 08 09 15 16 17 18

not examined

regional

not examined expression: see section 02 03 04 05 23 24

neural retina

moderate

regional

moderate expression: see section 02 03 04 23 24 weak expression: see section 01

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5904
Entity Detected:	Dhcr7, 7-dehydrocholesterol reductase ( MGI:1298378)
Sequence:	sense strand is shown >T5904 GGATTCCGCACTCAGGGCCTGATGGCTTCGAAATCCCAGCACAACGCTCCCAAAGTCAAGAGTCCCAACG GCAAGGCTGGATCTCAAGGACAATGGGGCCGTGCCTGGGAAGTGGACTGGTTCTCCCTGGCCAGCATCAT TTTCCTGCTGCTCTTCGCTCCATTCATTGTGTACTACTTCATCATGGCATGTGACCAGTACAGCTGCTCC CTGACTGCCCCCGCATTGGATATAGCTACAGGCCATGCTAGTCTGGCAGACATTTGGGCCAAGACACCAC CTGTGACAGCTAAAGCTGCCCAACTGTATGCCTTGTGGGTCAGCTTCCAGGTGCTGCTTTATTCCTGGCT TCCTGACTTCTGCCACAGATTTCTGCCAGGTTATGTGGGAGGCGTCCAAGAAGGTGCCATTACTCCAGCA GGGGTTGTAAACAAGTATGAGGTGAATGGGCTGCAAGCCTGGCTCATTACCCACATCCTCTGGTTTGTGA ACGCTTACCTCTTATCCTGGTTCTCCCCCACCATCATCTTTGACAACTGGATCCCCTTGCTATGGTGTGC CAACATCCTGGGCTATGCCGTGTCCACGTTCGCAATGATCAAGGGCTACCTGTTCCCCACCAGTGCCGAA GACTGCAAATTCACAGGCAATTTCTTCTACAACTATATGATGGGAATTGAGTTCAACCCCCGCATCGGGA AGTGGTTTGACTTCAAGCTGTTCTTCAATGGACGACCAGGGATTGTAGCCTGGACCCTCATTAACCTGTC CTTCGCTGCCAAGCAGCAGGAGCTTTATGGCCATGTGACCAACTCCATGATTTTGGTCAATGTCTTGCAG GCCATCTACGTGTTAGACTTCTTCTGGAATGAAACCTGGTACCTGAAGACCATCGACATCTGCCATGACC ACTTTGGGTGGTACCTAGGCTGGGGAGATTGTGTGTGGCTGCCCTACCTCTACACACTGCAGGGCCTGTA CTTGGTGTACCACCCTGTGCAGCTCTCCACCCCCAATGCCTTGGGCATCCTGCTGCTTGGCCTCGTGGGT TACTATATCTTCCGAATGACCAACCATCAGAAGGACCTGTTCCGCAGGACAGATGGCCGCTGCCTCATCT GGGGCAAGAAGCCCAAGGCCATCGAGTGCTCCTACACATCAGCTGATGGGCTGAAGCACCACAGCAAGCT GTTGGTCTCAGGCTTCTGGGGTGTGGCCCGCCACTTCAACTATACTGGTGACCTGATGGGCAGCCTTGCC TACTGCCTGGCTTGTGGCGGTGGCCACCTCCTCCCCTACTTTTACATCATCTATATGACTATCCTGCTCA CCCACCGCTGCCTCCGTGATGAACACCGCTGTGCCAACAAGTATGGCCGTGATTGGGAGCGCTACACAGC CGCTGTGCCCTACCGCCTGCTGCCTGGAATCTTCTGAGGGCAGCCTTGGGAAGCATAGAGAGGTGTCATT GAGTGTGTGGAGTTGGATGCATGGGGGCTGGCATCTGGCTCTCCCCTGCAAACTGCCTCCTCAGTTTGCT CATCTGGAGAATGGAGAGCCTGGTGTCCCACAGTGGCTGCTGACAGCTGGAGAGCTGAATTCACACGGAT ACCCCAGCAGAAAGGCTTGTTCTCTCTGTTCAGCATCCTGATAGCAGAGGCCCTTTCATGTCTCTCTGCT TGGAGCCCACCATCTATTCTGTCTAGATAACTTAGGCTTATCAGATGTCTCCGATGATTGGGCCATCCTG GTAAACCGGAGACCATGCAGGATTTAAAGAGTTTGGCAATGCCATCACCCCCCGCCGCCCCCCCCCCATC CTGGTGAGCTAGGCTCCATTTTTGTCTGTAGCATGTGCAGTGTTAATTGATCAGCCAAGGGTTCAGAGGC CATTGAAGAAGGGAGGCTTTTTTGTTTAGGTTCTTGATTTTTTGTTTCTTTGTTTGTTTTTGTTTTTACC CCAGGAATGGCAGACAGTGGACTGGAATATTCAGGGGGCACTGCCCACTGCACAGTCCCTGGCTAACTTG ATAAACAGGGTTCCCAGGACTCCCCAAGTGCCCACCTAGCAGCTCATCCACCTGCCCTCTTCTGGAAGGT GCTTCTTGCTTACCCCTGAGACTTGGCTTGGACAACCCAGAACGTATCACCAGTCAGTGGCCTTAGCCAC ACCTTCCCCAACTGGAGACCCAGTGTTGGGGAAGGAACACATTCTCATTAGGGACACTGCCCCCCCAAAC TCAGGGTATTCTGTAAAACTAGATAGGCTTATTTTAGCCTTGCAGGCAGTTCTTTGTATTAAATCTGCTT TGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3157987 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3157987 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7222 same embryo
	EMAGE:7221 same embryo
	EMAGE:7223 same embryo
	EMAGE:7220 same embryo
	EurExpress:euxassay_002715 same experiment
	MGI:4824296 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source