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EMAGE:724

Nkx2-2 NK2 transcription factor related, locus 2 (Drosophila) ( MGI:97347)
TS16 (10 dpc)
in situ hybridisation

Data Images
EMAGE:724 EMAGE:724 EMAGE:724 EMAGE:724
Figure 3G of Shimamura et al., 1995 [PMID:8575293] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 3I of Shimamura et al., 1995 [PMID:8575293] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 3J of Shimamura et al., 1995 [PMID:8575293] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 3K of Shimamura et al., 1995 [PMID:8575293] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Wholemount (dissected brain) is a double in situ; purple - Nkx2-2 expression; brown - Shh expression. The sections are taken from the planes shown on the wholemount image. Image annotations: me - mesencephalon; di - diencephalon; sp - secondary prosencephalon; os - optic stalk; is - isthmus; rh - rhombencephalon; large arrow points to the midline just ventral to the optic stalk; large arrowhead points to zona limitans; small arrowhead interrhombomeric boundaries; open arrow is the rostral limit of Shh expression at the ventral midline; small arrow points to the medial ganglionic eminence.
Expression Pattern Description
Spatial Annotation:
EMAGE:724Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
724_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:724_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
central nervous system
detected detected
Expression in a stripe along the entire anterior-posterior extent of the central nervous system.
future brain
detected detected
regionalExpression has a dorsal deflection at the zona limitans (large arrowhead). Expression crosses the midline just ventral to the optic stalk (large arrow). Anterior to the isthmus the expression extends to the front of the brain in a more dorsal position.
future hindbrain
detected detected
regionalExpression has dorsal deflections at the interrhombomeric boundaries (small arrowheads).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1277654
Entity Detected:Nkx2-2, NK2 transcription factor related, locus 2 (Drosophila) ( MGI:97347)
Notes:The Nkx2-2 probe used in this study by Shimamura et al., 1995 [PMID:8575293] is described as a "2,026bp cDNA". Exact probe end points are undefined.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Shimamura et al., 1995 [PMID:8575293] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8575293] Shimamura K, Hartigan DJ, Martinez S, Puelles L, Rubenstein JL 1995 Longitudinal organization of the anterior neural plate and neural tube. Development (121):3923-33
Links:MGI:1934636 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE