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EMAGE:726

Wnt8b wingless related MMTV integration site 8b ( MGI:109485)
TS15 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:726
Figure 6A of Varlet et al., 1997 [PMID:9056778] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a "control chimeric embryo, created by injection of wild type ES cells into wild type embryo". Image annotations: ov - otic vesicle; op - optic pit; h - heart; hb - hindbrain; mb - midbrain; di - diencephalon; tel - telencephalon; pink arrowhead indicates pale pink staining of Krox20 (Egr2). Only the purple Wnt8b staining has been spatially mapped in this EMAGE entry.
Expression Pattern Description
Spatial Annotation:
EMAGE:726Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
726_wholemount_strong_3D_1.wlz
726_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:726_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalWnt8b is expressed dorsally
diencephalon
detected detected
regionalExpression is rostral and dorsal.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1335317
Entity Detected:Wnt8b, wingless related MMTV integration site 8b ( MGI:109485)
Notes:The Wnt8b probe used in this study by Varlet et al., 1997 [PMID:9056778] was "kindly provided by Scott Lee and Andrew McMahon (S.Lee and A.McMahon, personal communication)" and is of unspecified sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase
General Information
Authors:Varlet et al., 1997 [PMID:9056778] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9056778] Varlet I, Collignon J, Robertson EJ 1997 nodal expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation. Development (124):1033-44
Links:MGI:1335322 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE