EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7281

Stt3a STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) ( MGI:105124)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004591. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004591_01	euxassay_004591_02	euxassay_004591_03	euxassay_004591_04

euxassay_004591_05	euxassay_004591_06	euxassay_004591_07	euxassay_004591_08	euxassay_004591_09

euxassay_004591_10	euxassay_004591_11	euxassay_004591_12	euxassay_004591_13	euxassay_004591_14

euxassay_004591_15	euxassay_004591_16	euxassay_004591_17	euxassay_004591_18	euxassay_004591_19

euxassay_004591_20	euxassay_004591_21	euxassay_004591_22	euxassay_004591_23	euxassay_004591_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7281_wholemount_strong.wlz

7281_wholemount_moderate.wlz

7281_wholemount_weak.wlz

7281_wholemount_possible.wlz

7281_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7281_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 20 21 22 23 24

viscerocranium

moderate

regional

Expression in the turbinate bone.

mandible

moderate

regional

moderate expression: see section 03 04 07 08 09 10 13 14 15 16 17 18 19 20 21 22 weak expression: see section 05 06

maxilla

moderate

regional

moderate expression: see section 07 08 09 10 13 14 15 16 17 18 19 weak expression: see section 05 06

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 20 21 22 23 24

clavicle

moderate

regional

moderate expression: see section 09 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7648
Entity Detected:	Stt3a, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) ( MGI:105124)
Sequence:	sense strand is shown >T7648 AAGAGGTAATCCCGGATGGCAGGGGGAGCAGAGTGGGTTCCTGAGGAGCATCCGTGAGGTATTTGAATAT CATCAGTTGCCACCCATTGATGTCAAGATGACTAAGCTTGGATTTTTGCGATTGTCCTATGAGAAGCAGG ACACACTTCTAAAGCTTCTCATCTTGTCGATGGCTGCTGTGTTATCTTTTTCTACTCGTCTTTTTGCTGT GCTGAGATTTGAAAGTGTCATCCATGAGTTTGATCCGTACTTTAATTATCGGACTACCCGGTTTCTGGCT GAGGAGGGGTTTTATAAATTCCATAACTGGTTTGATGACCGGGCTTGGTACCCTTTGGGCCGAATCATTG GAGGAACAATTTACCCAGGTTTAATGATCACTTCTGCTGCAATCTACCATGTACTCCATTTCTTCCATAT CACCATTGACATTCGGAATGTCTGTGTTTTCCTGGCCCCGCTTTTCTCCTCTTTCACCACCATCGTTACG TACCACCTTACCAAAGAGCTCAAGGATGCAGGAGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTC CTGGGTATATTTCTCGATCTGTAGCTGGCTCCTATGATAATGAAGGAATTGCTATCTTTTGCATGCTGCT TACTTACTACATGTGGATCAAGGCAGTGAAGACTGGTTCCATCTATTGGGCTGCCAAGTGTGCCCTCGCT TATTTCTACATGGTCTCTTCATGGGGAGGCTATGTGTTCCTGATCAACTTGATTCCTCTACATGTCCTGG TGCTAATGCTGACAGGCCGTTTTTCTCACCGGATCTACGTAGCCTACTGTACTGTTTACTGCCTGGGCAC CATTCTTTCTATGCAGATTTCCTTTGTTGGTTTCCAGCCCGTCCTTTCATCAGAACACATGGCGGCCTTT GGAGTGTTTGGTCTCTGTCAGATCCATGCTTTCGTAGATTACCTGCGCAGCAAGTTGAATCCACAGCAAT TCGAAGTTCTTTTCCGGAGTGTTATCTCCCTGGTTGGCTTTGTCCTCCTCACTGTGGGAGCTCTCCTCAT GCTAACAGGAAAAATTTCTCCCTGGACAGGGCGTTTCTACTCTCTGCTGGATCCCTCTTATGCTAAGAAT AACATTCCCATTATTGCATCTGTTTCTGAGCACCAGCCCACAACCTGGTCTTCCTACTATTTTGATCTAC AGCTCCTTGTCTTCATGTTTCCAGTTGGCCTCTATTACTGCTTTAGCAACCTGTCTGATGCTCGGATTTT TATCATCATGTATGGTGTGACCAGCATGTACTTTTCAGCTGTAATGGTGCGTCTAATGCCGGTATTGGCA CCTGTTATGTGCATTCTTTCTGGCATTGGTGTTTCCCAGGTGCTGTCCACATATATGAAAAATCTGGACA TAAGTCGCCCAGACAAGAAGAGCAAGAAGCAACAGGATTCTACTTACCCTATTAAGAATGAGGTGGCGAG TGGGATGATACTGGTCATGGCTTTCTTTCTCATCACCTACACGTTTCATTCGACTTGGGTGACCAGTGAA GCCTATTCTTCTCCCTCCATTGTTCTGTCTGCTCGTGGTGGGGATGGCAGTAGGATCATTTTTGATGACT TCCGAGAAGCGTATTATTGGCTCCGTCACAATACTCCAGAGGATGCAAAAGTCATGTCATGGTGGGATTA TGGCTACCAAATTACTGCAATGGCAAATCGGACAATTTTAGTGGACAATAACACATGGAATAATACCCAT ATTTCTCGAGTAGGGCAGGCAATGGCATCCACAGAAGAAAAAGCCTATGAAATCATGAGGGAGCTTGATG TCAGCTATGTGCTTGTCATTTTTGGAGGCCTTACTGGGTATTCTTCGGATGATATCAACAAGTTTCTTTG GATGGTCCGGATTGGAGGAAGCACAGAGACAGGAAGACACATTAAGGAGAATGACTACTATACTCCTACT GGGGAATTCCGTGTTGATCGTGAGGGTTCTCCGGTGCTGCTCAACTGCCTTATGTACAAAATGTGTTACT ACCGCTTTGGGCAGGTCTACACAGAAGCCAAGCGTCCACCAGGCTTTGACCGTGTTCGAAATGCTGAGAT TGGTAATAAAGACTTTGAGCTTGATGTCCTGGAGGAAGCGTATACCACAGAACACTGGCTAGTCAGGATA TACAAGGTAAAGGACCTGGATAATCGAGGCTTGTCAAGGACATAAACGTCACATTGTGCCCTGAGCATTA TGCTTCGCACTGAGCGCGTCATGTTGAGGACGCTGAAGATGTTTTTTATATGCAGTTTATAAGAACAGCC GGATGGGGTTAGAATTGTCTGCAAGTTTTGCCCTGGACAATATGGGCTGGGCCAAGTGAAATGATTTTTA TAATTCTGAGCAGGTTACCAAATGAAATGTTATGGCTTTACTTTGGTCAATTAAAAGGGGGGGGGGATTT TTTTTAAATGTGCCTTATTTGTTTTGACTTAAATTGGCTGATACGAGGATCACAGAAGTGAGCGGATGGA AGACCATATCCATGCTCTAGGTCCCCAAATGAACCAGATAGGAGCATTTTTTTCTCCTATCAGCAATCTC AAGGACTAGCTCTGGTTCAACAAATGTAAACAACAACTTTGTCACACTTTTTTGTTTTTTAGCACCCAGG TACAATGCTTTCCTTATAATGGGTGCTTAATAAATTTTTATCAAATGAATAAATGTTTCTGGGACCAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30443880 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30443880 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7283 same embryo
	EMAGE:7284 same embryo
	EMAGE:7282 same embryo
	EurExpress:euxassay_004591 same experiment
	MGI:4828520 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source