EMAGE mouse gene expression spatial database

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EMAGE:7283

Hmgb1 high mobility group box 1 ( MGI:96113)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004493. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004493_01	euxassay_004493_02	euxassay_004493_03	euxassay_004493_04

euxassay_004493_05	euxassay_004493_06	euxassay_004493_07	euxassay_004493_08	euxassay_004493_09

euxassay_004493_10	euxassay_004493_11	euxassay_004493_12	euxassay_004493_13	euxassay_004493_14

euxassay_004493_15	euxassay_004493_16	euxassay_004493_17	euxassay_004493_18	euxassay_004493_19

euxassay_004493_20	euxassay_004493_21	euxassay_004493_22	euxassay_004493_23	euxassay_004493_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7283_wholemount_strong.wlz

7283_wholemount_moderate.wlz

7283_wholemount_weak.wlz

7283_wholemount_possible.wlz

7283_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7283_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6466
Entity Detected:	Hmgb1, high mobility group box 1 ( MGI:96113)
Sequence:	sense strand is shown >T6466 CGGACGCGTGGGTCCCGCTCTCACAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAG CGCGAGCCGGACGGGCACTGGGCGACTCTGTGCCTCGCGGAGGAAAATCAACTAAACATGGGCAAAGGAG ATCCTAAGAAGCCGAGAGGCAAAATGTCCTCATATGCATTCTTTGTGCAAACTTGCCGGGAGGAGCACAA GAAGAAGCACCCGGATGCTTCTGTCAACTTCTCAGAGTTCTCCAAGAAGTGCTCAGAGAGGTGGAAGACC ATGTCTGCTAAAGAAAAGGGGAAATTTGAAGATATGGCAAAGGCTGACAAGGCTCGTTATGAAAGAGAAA TGAAAACCTACATCCCCCCCAAAGGGGAGACCAAAAAGAAGTTCAAGGACCCCAATGCACCCAAGAGGCC TCCTTCGGCCTTCTTCTTGTTCTGTTCTGAGTACCGCCCCAAAATCAAAGGCGAGCATCCTGGCTTATCC ATTGGTGATGTTGCAAAGAAACTAGGAGAGATGTGGAACAACACTGCAGCAGATGACAAGCAGCCCTATG AGAAGAAAGCTGCCAAGCTGAAGGAGAAGTATGAGAAGGATATTGCTGCCTACAGAGCTAAAGGAAAACC TGATGCAGCGAAAAAGGGGGTGGTCAAGGCTGAAAAGAGCAAGAAAAAGAAGGAAGAGGAAGATGATGAG GAGGATGAAGAGGATGAGGAAGAGGAGGAAGAAGAGGAAGACGAAGATGAAGAAGAAGATGATGATGATG AATAAGTTGGTTCTAGCGCAGTTTTTTTTCTTGTCTATAAAGCATTTAACCCCCCTGTACACAACTCACT CCTTTTAAAGAAAAAAATTGAAATGTAAGGCTGTGTAAGATTTGTTTTTAAACTGTACAGTGTCTTTTTT TGTATAGTTAACACACTACCGAATGTGTCTTTAGATAGCCCTGTCCTGGTGGTATTTTCAATAGCCACTA ACCTTGCCTGGTACAGTCTGGGGGTTGTAAATTGGCATGGAAATTTAAAGCAGGTTCTTGTTGGTGCACA GCACAAATTAGTTATATATGGGGACAGTAGTTTGGTTTTTTGTTTTGTTTTTTTCTTTTGGTTTTCTTTC TGGGTTTTATTTTTTTCATCTTCAGTTGTCTCTGATGCAGCTTATACGAAGATAATTGTTGTTCTGTTAA CTGAATACCACTCTGTAATTGCAAAAAAAAAATTGCGGCTGTTTTGTTGACATTCTGAATGCTTCTAAGT AAATACAATTTTTTTTATTAGTATTGTTGTCCTTTTCATAGGTCTGAAAGTTTTCTTCTCGAGGGGAAGC TAGTCTTTTGCTTTGCCCATTTTGGGTCACATGGATTATTAGTGTGTTATCTTTCATCTAGTTAGCTGGA AGAGAGCTTTTGTCCACATGCCCTGCCATTGTGGTAGGGTAACATTTTCATCCATAGTTGAAGAATCTCC TAAATTGTGATAGTTGGATAAGAGATATTATATAACCTACTTGGCAAAGCAAGGAGTGATCAATACTGTC ACACCGTGGGACTATTAGGATCAAGCAATCTGAACGTCTGTCCTTGAAGGACTGATGGAAAAGTACCTTC TAATCCTTACACGAGGACTCTCCTTTAACCGCCATTACTGTGTAATGACAGTTATATTTTGCAGTTTCCC CTACTAAAGAAGACCTGAGAATGTATCCCCAAAAGTGTGAGCTTAAAATACAAGACTGCTGTACTATTTG TTGACCTTAGTCCCAGCGAAGGCTATCACAAGAACGCTGGCTGTAAAGCCTTTGCCCTTCTATCTAGATA TGGATTGCTCAGGAAACTTGACTGTTTAAAGGTATTTTTAATTACTTGAGCCAGCTTTTAAAATTATGCC ACATTTAAAATGAAGGGTATATTTTCCTATACTGTGGTTTGTCCCTTTATGAATCAGATACAAGAGGATA AACTTTGCATATTAGTACCATTTGTCCAATACATTTGCTTTTTCTTTATAAAACCCAAACTCATTCATTA ATCAGGTTTAATCTGCTTAGTTTAGGGAACAATTTGGCAATTTTGTGGATTTTTTTTTGAGATTATCGTT CTCTTAAAGTGCCAGTGTTTTAAATAGCGTTCTTGTAATTTCACGCGCTTTTGTGATGGAGTGCTGTTAT ATAATTTTGACTTGGGTTCTTTACATTTGCGTTGTTAATGTAATTTGAGGAGGAATACTGAACATGAGTC CTGGATGATACTAATAAACTAATAATTACAGAGGTTTTAAATATTAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3586745 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3586745 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7284 same embryo
	EMAGE:7282 same embryo
	EMAGE:7281 same embryo
	EurExpress:euxassay_004493 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source