EMAGE mouse gene expression spatial database

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EMAGE:7284

Stk17b serine/threonine kinase 17b (apoptosis-inducing) ( MGI:2138162)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004505. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004505_01	euxassay_004505_02	euxassay_004505_03	euxassay_004505_04

euxassay_004505_05	euxassay_004505_06	euxassay_004505_07	euxassay_004505_08	euxassay_004505_09

euxassay_004505_10	euxassay_004505_11	euxassay_004505_12	euxassay_004505_13	euxassay_004505_14

euxassay_004505_15	euxassay_004505_16	euxassay_004505_17	euxassay_004505_18	euxassay_004505_19

euxassay_004505_20	euxassay_004505_21	euxassay_004505_22	euxassay_004505_23	euxassay_004505_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7284_wholemount_strong.wlz

7284_wholemount_moderate.wlz

7284_wholemount_weak.wlz

7284_wholemount_possible.wlz

7284_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7284_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6464
Entity Detected:	Stk17b, serine/threonine kinase 17b (apoptosis-inducing) ( MGI:2138162)
Sequence:	sense strand is shown >T6464 GTAAGAAGCGTCGGTCAGCGTCTGTGCGGTCGCCGCCGGGAGTCGCCTCACAGGGGCCTGGCTGACGGCG ACCAGCCGTTGTGGGGAAGAGTGCGAGGTAAAAGTCTGCCTAGAGAAGCAGGTCTGGCAGTCATCAACAT GTCTCGGAGGAGATTCGATTGCCGAAGTGTCTCAGGCTTGCTAACTACAACCCCTCAAACGCCGATGAAA ACAGAGAATTTTAATAATTTCTATACTCTTACCCCAAAAGAACTTGGGAGAGGAAAATTTGCTGTGGTTA GACAATGTATATCAAAATCAACTGGACAAGAGTATGCTGCCAAATCCCTGAAGAAGAGGAGACGAGGGCA GGATTGCCGGGCGGAGATTCTGCATGAGATAGCTGTGCTGGAGCTGGCCAGGTCTTGTCCCCACGTGATT AATCTGCATGAGGTCTACGAAAATGCAACGGAAATCATTTTGGTGTTAGAATATGCTGCGGGTGGAGAAA TTTTCAACCTGTGTTTACCTGAGTTAGCCGAAATGGTATCTGAAAATGATGTTATCAGACTCATTAAACA AATCCTTGAAGGAGTTCATTATCTACATCAGAATAACATTGTTCACCTTGATTTAAAGCCACAGAATATA CTTTTGAGCAGTATATACCCACTCGGGGACATAAAAATTGTAGATTTTGGAATGTCTCGAAAAATTGGGA ATGCAAGTGAGCTTCGGGAAATCATGGGAACACCTGAATACTTAGCTCCAGAAATCCTCAACTATGATCC CATTACCACAGCAACAGATATGTGGAATATTGGCATAATAGCGTATATGTTGTTAACTCATACGTCACCA TTTGTAGGAGAAGATAATCAAGAAACATATCTGAATATTTCTCAAGTGAATGTAGATTATTCAGAAGAAA TGTTTTCATCAGTTTCACAGCTGGCCACAGACTTCATCCAGAGCCTTCTAGTAAAGAACCCAGAGAAAAG ACCAACAGCAGAATCCTGCCTATCCCACTCATGGCTGCAGCAGTGGGACTTTGGAAGCTTGTTTCATCCT GAGGAAACTTCAGGCTCCTCTCAAATTCAGGATCTGACTCTCAGGTCCTCTGAAGAGAAGACCTCCAAGT CCTCCTGTAATGGGAGCTGTGGAGCCCGGGAGGACAAGGAGAACATCCCTGAAGATGGCAGCTTAGTTTC TAAAAGATTTCGATTCGATGACTCCTTGCCCAGCCCCCATGAACTTGTTCCAGATTTGTTCTGTTAGCAT TTTTCTCTGTGACTCATCTGGACTGACTCGGAAATTTGAAATCTCTGGTGTGAGATTGTGTTTGTAGCTT CATATATTATGTTTATATTATAAATGCACTTCTGCTTAGAAGAACTTAAGGAACAGTTTAAATGCTAGGC TTCTGTTGGCTAGCATATCATTTCTTGTCCTGAAATTGTTTTGCAGAGGAAAATATTTAAGTATATGACA AAAAATGTAAATTGTGTTTAAGAGAACACATGCAACTGAAAGAACTCAAGTTCAGTCAGACTTATAAAAT GGGTTATATTATGGTTAGTAAAAGTTGAAAAAAAAATGAAAACAGGAATTTAGTAGGTTCTAAGGTAAGC CCTATACCATAACTCTATTACAGAGAATCTGTTTGGGGAAATGCTGTCAAGGGTAAACCACAACATATAC TGCTTTATAAATACTCCAGAGAGAGTTTATAATTGAAAGTATTTCCCAGTTACCAATAATAGCTTGAAAC TGTAAGATTTTCTTTGTGTGCCATGTGCTCGGTGAGAGGACACAGTCAACCAGAGCAGGGTTGATCCAGG CTGTTTCTCTGCAAACCGAGTCAAAACTCGACATCATTTCCAGCTCATGTATTTTGTACGTGCATCATAT ATCAGATCTAATAAGATCTGGAAGATGGATATGCAAATAAGAGGCCTTTGTCTTCTAGAATGATTAGAGT AGAGGAGAATTGGATAGTACAGAATATGCTCTAGTTTCAGTCAGACATATTCATAAAGGGAAATGTTAAG TTCTGGCAGCTGACTTAGTGTTGGATGTCTCCTAAGTCTCAGGATAGAAGCCCATCATTAGAGCATAGGC ACTTCAGGAATTCTTGTGTGAAATTCTAGTGAGTGAGGAGGTGTGACATGCAGCTATCTTTGGGCTCCTT TTGTGTGTGTTCTGCTGGACACAACACATGGGAGTGTTCAGTGTTGTCCATGGTCAATATCTATGTTCAG TCCTGATGGGAGGGGCCTAGGGACTGCTTTGGAGATTTCCCACTGGTGTCCATTTTAAGGTCTGTAATAA TGTCAAGATAACAGATCTCATAAATATGCTACTCTATCAGACTCCGTCGCCAAAACAAATTAAAAGCCTG TGTATTGAAGTGGGTGTTAGTCTAACAACCTGTAAATTCTTGAAATTGTTACTAAAATTCCAAATTCTTT AGATAACTTTAAACTATTTAAATTGAGCATTGCTGTCTTTGTTTGATTAAAGGTTGAGTTCCTTTATATC TGTTATTTTTAAAGGAAAAGTTGTTCGCCTTTTGTATATGTGTGTGCATATGTGTATGTGTACAGGTATA TGTATATATGTATTGATAGATAAAATACAGCCTTTAAACAACTTCAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3586767 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3586767 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7283 same embryo
	EMAGE:7282 same embryo
	EMAGE:7281 same embryo
	EurExpress:euxassay_004505 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source