Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:729

Shh sonic hedgehog ( MGI:98297)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:729
Figure 2F of Rallis et al., 2003 [PMID:12736217] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: FL - forelimb; HL - hindlimb; arrow indicates Shh expression in the ZPA (zone of polarising activity) of the forelimb.
Expression Pattern Description
Spatial Annotation:
EMAGE:729Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
729_wholemount_strong_3D_1.wlz
729_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:729_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud mesenchyme
detected detected
regionalExpression in the zone of polarizing activity (ZPA) of the forelimb and hindlimb.
hindlimb bud mesenchyme
detected detected
regionalExpression in the zone of polarizing activity (ZPA) of the forelimb and hindlimb.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23359
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Rallis et al., 2003 [PMID:12736217] was previously described by Echelard et al., 1993 [PMID:7916661] as "either a 2.8 kb or 0.6 kb RNA transcript generated by T7 (2.8 kb) or T3 (0.6 kb) transcription of XbaI and HindIII digests of Bluescript (Stratagene) subclones of the original Shh cDNA inserts."
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Rallis et al., 2003 [PMID:12736217] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00473] [ PMID:12736217] Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ, Logan MP 2003 Tbx5 is required for forelimb bud formation and continued outgrowth. Development (130):2741-51
 [ doi:10.1016/0092-8674(93)90627-3] [ PMID:7916661] Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP 1993 Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell (75):1417-30
Links:MGI:2677741 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI