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EMAGE:73

En1 engrailed 1 ( MGI:95389)
TS15 (9.7 dpc)
in situ hybridisation

Data Images
EMAGE:73
Figure 4E of Martinez-Barbera et al., 2001 [PMID:11731459] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:73Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
73_wholemount_strong_3D_1.wlz
73_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:73_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:En1_probeB
Entity Detected:En1, engrailed 1 ( MGI:95389)
Sequence:sense strand is shown

>En1_probeB
AACATCCTAAGGCCCGATTTCGGTTGCAAAAAGGAACAGCCCCTGCCTCAGCTCCTGGTGGCTTCGGCTG
CAGCCGGAGGAGGCGCAGCAGCAGGAGGAGGAAGCCGCGTGGAGCGTGACCGAGGCCAGACTGGTGCAGG
TAGAGACCCCGTTCACTCTCTGGGCACACGAGCTTCGGGGGCTGCCTCGCTCTTGTGTGCTCCAGATGCG
AACTGTGGCCCACCCGACGGCTCCCAGCCCGCCACCGCTGTCGGCGCCGGCGCATCCAAAGCCGGGAACC
CGGCTGCTGCGGCGGCCGCGGCCGCAGCAGCGGCTGCAGCGGCAGTGGCGGCAGCGGCGGCAGCAGCCTC
GAAGCCCTCGGACAGTGGCGGTGGTAGTGGAGGCAACGCGGGGAGTCCCGGGGCGCAGGGCGCCAAGTTC
CCGGAACACAACCCTGCGATCCTAC
nt 689 - nt 1133 of NM_010133.1
Notes:Probe used in this study by Martinez-Barbera et al., 2001 [PMID:11731459] is indicated as being described by Acampora et al. 1998 [PMID:9811592] ie. a "PCR fragment including the region between aa 112 and aa 260 (Logan et al., 1992 [PMID:1363401] )".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6 x DBA/2
Age:9.7 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + undefined
General Information
Authors:Martinez-Barbera et al., 2001 [PMID:11731459] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11731459] Martinez-Barbera JP, Signore M, Boyl PP, Puelles E, Acampora D, Gogoi R, Schubert F, Lumsden A, Simeone A 2001 Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2. Development (128):4789-800
 [ PMID:9811592] Acampora D, Avantaggiato V, Tuorto F, Briata P, Corte G, Simeone A 1998 Visceral endoderm-restricted translation of Otx1 mediates recovery of Otx2 requirements for specification of anterior neural plate and normal gastrulation. Development (125):5091-104
 [ doi:10.1002/dvg.1020130505] [ PMID:1363401] Logan C, Hanks MC, Noble-Topham S, Nallainathan D, Provart NJ, Joyner AL 1992 Cloning and sequence comparison of the mouse, human, and chicken engrailed genes reveal potential functional domains and regulatory regions. Dev Genet (13):345-58
Links:MGI:2155571 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI