EMAGE mouse gene expression spatial database

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EMAGE:7306

Sec16a SEC16 homolog A (S. cerevisiae) ( MGI:2139207)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004351. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004351_01	euxassay_004351_02	euxassay_004351_03	euxassay_004351_04

euxassay_004351_05	euxassay_004351_06	euxassay_004351_07	euxassay_004351_08	euxassay_004351_09

euxassay_004351_10	euxassay_004351_11	euxassay_004351_12	euxassay_004351_13	euxassay_004351_14

euxassay_004351_15	euxassay_004351_16	euxassay_004351_17	euxassay_004351_18	euxassay_004351_19

euxassay_004351_20	euxassay_004351_21	euxassay_004351_22	euxassay_004351_23	euxassay_004351_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7306_wholemount_strong.wlz

7306_wholemount_moderate.wlz

7306_wholemount_weak.wlz

7306_wholemount_possible.wlz

7306_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7306_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5263
Entity Detected:	Sec16a, SEC16 homolog A (S. cerevisiae) ( MGI:2139207)
Sequence:	sense strand is shown >T5263 GACCATAGAACTGTATGGCTTCCTGGGAAGTCACCCAATGAGGCAAACCTGATAGATTTTACCAATGAGG CTGTGGAACAAGTGGAAGAGGAGGAGTCAGGGGAGGCCCAGCTCTCATTTCTCACAGACAGTCAGACAGT GACCACCAGCGTGCTGGAGAAGGAGACTGAGCGGTTCCGTGAGCTCTTGCTGTATGGCCGGAAGAAGGAT GCTCTGGAGTCTGCCATGAAAAATGGCTTATGGGGTCATGCTCTTCTGCTTGCAAGTAAGATGGACAGCC GGACACATGCCCGTGTCATGACCAGGTTTGCCAACAGTCTTCCAATCAATGACCCCCTGCAGACTGTCTA CCAGCTCATGTCTGGACGGATGCCTGCTGCATCCACGTGTTGTGGCGATGAGAAGTGGGGAGATTGGAGG CCACATCTTGCAATGGTTTTGTCCAACCTGAACAACAACATGGATGTTGAATCTAGGACCATGGCTACGA TGGGTGATACTCTAGCATCAAAAGGCCTGTTGGATGCTGCACACTTCTGCTACCTGATGGCCCAGGTTGG ATTTGGGGTTTATACAAAGAAAACTACAAAACTTGTCTTGATAGGCTCAAATCACAGTTTGCCCTTTTTA AAGTTTGCCACCAATGAAGCCATTCAAAGGACGGAGGCTTATGAGTATGCTCAGTCCCTTGGGGCACATA CCTGTTCCTTACCTAACTTCCAGGTGTTTAAATTCATCTACTTGTGCCGCCTGGCTGAAATGGGACTTGC CACACAGGCCTTCCACTACTGCGAAGTAATAGCCAAGAGTGTCCTGACACAGCCTGGTGCATACTCTCCA GTACTGATTAGCCAGTTGACTCAGATGGCTTCCCAGTTACGCCTCTTCGATCCTCAACTGAAGGAGAAGC CAGAAGAGGAGTCCTTTGTGGAGCCTGCCTGGTTGGTGCAGCTGCAGCACGTGGAGAGGCAGATCCAGGA GGGCACTGTGCTGTGGAGCCAGGATGGAACTGAACCCCAGCAGTGTCGCATTACATCGGGCTCTGAGGTG GAGCAGTCAGATGGCCCTGGACTCAACCAGCAGGCAGGGCCACAGGCCGACAACCCTCTACTGATGCCAA GCACTGAGCCTTTGATGCATGGCGTGCAACTGCTGCCCACAGCTCCTCAGACATTGCCTGATGGCCAGCC TGCTCACCTTTCCAGGGTGCCGATGTTCCCAGTGCCAATGTCTCGTGGGCCCCTGGAGCTGAGTCCTGCC TATGGACCCCCAGGATCTGCACTTGGCTTCCCAGAATCCTCCAGATCTGATCCTGCAGTGCTGCATCCTG GGCAGGCCCTGCCACCCACTACACTAAGTCTCCAGGAAAGTGGGCTTCCACCCCAGGAGGCCAAAAGTCC AGACCCAGAAATGGTGCCACGGGGCTCACCTGTCAGACACTCCCCTCCGGAGCTCAGTCAAGAGGAGTTT GGCGAGAGCTTCGCTGACCCGGGCTCTTCCAGAACAGCACAGGACTTGGAAACCTCCCCAGTGTGGGATC TTGGCAGCTCCAGTTTGACACGTGCACCATCTTTGACATCTGATTCTGAAGGGAAGAAACCTGCACAGGC TGTCAAAAAGGAGCCCAAGGAGCCCAAGAAGACTGAGTCCTGGTTCTCTCGTTGGCTGCCTGGAAAAAAA AGGACAGAAGCTTATCTACCAGATGACAAGAACAAATCAATTGTTTGGGATGAAAAGAAAAACCAGTGGG TGAATTTGAATGAACCAGAGGAGGAGAAGAAGGCTCCACCCCCACCTCCAACATCGTTCCCCAGGGTTCC CCAGGTGGCTCCCACTGGGCCTGCAGGACCACCCACGGCCTCCGTGAATGTGTTTTCTAGAAAAGCAGGT GGGTCCAGAGCTCGCTATGTGGATGTTCTAAACCCAAGTGGAACACAGCGGAGTGAACCAGCTCTTGCTC CTGCAGATTTCTTTGCTCCTCTCGCCCCACTGCCAATTCCTTCTAACTTATTTGTACCAAACCCAGATGC AGAAGAGCCACAACCTGCAGATGGGACTGGCTGTAGGGGACAGGCACCAGCTGGGACTCAGTCTAAGGCA GAGTCCACCCTGGAACCCAAGGTGGGAAGTTCTACAGTATCAGCCCCTGGACCTGAGCTCTTACCCTCCA AACCAGATGGCTCCCAAGGAGGAGAGCTCTCGCGTTGTAGCTCTCTGAGCTCGCTCTCACAGGAAGTGAG CCGGCATTTCCATCAGGCTCCTGGAGACCATTGTCCTACAGGGGCCCCTCATGGTGGGTCTGTGCCCTTT TACAACCCTGCTCAGTTGGTGCAGGCTTCTGTCACCTCGGGAAATTCAAGGCCAGGGAGGATTGGCCAAA GAAAATATGCGGCATTGAACTAGGATTGCTGCTGGATTCTTATTTGGAACCCTGAGATGATTAGATAACT GTTCTTCACCAAGAACCAACTAGCTGCCTCAGGTCTCGTGGATCCCAGATGGACATGGCAGTAACTCAGA GCCGGTCATACTGTGTGTCTCCTTCCTTGCAGCCTATGTATATGAATGATTGAAAAATTGATTTCAAGGT TCCTTTCAAATGCTGGATAGAATGAATCTAATTCAAAGTCTTTTATGTAAAAGAACAATGTTTCTTTTTT AGTCACTTTAGACAAATTAAGGTTTGAGCCACTTGAGAATATCCCCGCAGAGAGGCTAGGCAGGGATTCA CTACAGGAGAGCACAGTTACTTGGACACCAAGGCCTTTCTCACCTGAGAAATGGTGCAAACCAACCAGGG ATCCTTAGGCTACTGAGAAGGCTGTCTGCTTACCTGGAGAGGACTGACTGCTGGGATGTCTGGCCTAAGA GGAGCCTCAAACTGCACTGGGCAACTGTGCCTTGTCCTGTGAACTGGGGAGTACATGCCAGCTCCTCAAA GGGTCACCAGATGGCAGCGGCTCCCAGGCTGGGGAGGAAGTGGTTACAAGGGCATGTCTCTCCAACATTT CCAGCCTCACTCCATTTTCTGTGGCCTCATTAGTGCTGGGTGCGTTTTTTTTTTGCTCCGAGCTTTCCGT CATGGCTCAGTCACTGGGGAGAGCCCCAGGGAGCTGATATTCTGAAGCTGCTGATCTGATTAACCAGGTC AAAAACTGGCTGTGTGCTGCAACGTACTGCCCTCCTAGCCAGGAGTCTGTATGGAGCCCTCTAGCTGACT CAATGTCACCTGCAGACAGCACTTAAAGCCTTGGTGGTATCTTGGAGTGGCTTACGGTGTCCTAGCGCAC ACTCTGGAACCTTGCATCTCTACCCCAAAGCCCACCAGGGACCTTCAGCAGCGACTCACTGGCTCACCCA TATCACTTCAAGGAGCTGCCACCACATAAGAGTCAAAGCTTTAAGGCTTCACTGACTGTTCCTAAAACTA TCCTTACACTCTAAAGAGGAAGGGAAAGAGAGTTAAATATTTAAAAATCAGCAGGTAGAAAACATAAATG GTTTGTTTGGTATTAAATTTAGAAATTGTAGAACATGGAATTAGTACAGCCCTTCCCGGTTTTCTGGTTC CTTGCATCATCTAGGAAAGACTCACAGAATGGCTTCCAGGACGTTCCATAGATGAGTGTACTTGGACCCT CCTGTTCCTGTCATTAAGAAAGGCCTGTCCCTGTGGGTCAGTAGCAACTTGTGTCCTTGCTGGGACCAGG CAGGACCCTTGCCTCTGGTTCAACCCTGCCCAGGCTCTGTGTTGACTGCCAACCACTTTGGATTCAAGAA GCTTCCTCCCTGGGAGCTGCTATTTTTCCAAAATTCACATTGTTAAATAAAATATTTTGGGCTCTCAAGT AAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5360092 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5360092 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7309 same embryo
	EMAGE:7307 same embryo
	EMAGE:7308 same embryo
	EMAGE:7304 same embryo
	EMAGE:7305 same embryo
	EurExpress:euxassay_004351 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source