EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7309

Gpcpd1 glycerophosphocholine phosphodiesterase GDE1 homolog (S. cerevisiae) ( MGI:104898)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004349. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004349_01	euxassay_004349_02	euxassay_004349_03	euxassay_004349_04

euxassay_004349_05	euxassay_004349_06	euxassay_004349_07	euxassay_004349_08	euxassay_004349_09

euxassay_004349_10	euxassay_004349_11	euxassay_004349_12	euxassay_004349_13	euxassay_004349_14

euxassay_004349_15	euxassay_004349_16	euxassay_004349_17	euxassay_004349_18	euxassay_004349_19

euxassay_004349_20	euxassay_004349_21	euxassay_004349_22	euxassay_004349_23	euxassay_004349_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7309_wholemount_strong.wlz

7309_wholemount_moderate.wlz

7309_wholemount_weak.wlz

7309_wholemount_possible.wlz

7309_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7309_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5241
Entity Detected:	Gpcpd1, glycerophosphocholine phosphodiesterase GDE1 homolog (S. cerevisiae) ( MGI:104898)
Sequence:	sense strand is shown >T5241 CTGTAACTGGGCTCCTTGCTTCCTCGGGCCACCTAAGGGAGCGCTGGCGAGGCGCTGACGCTCGGCTCCG TCGGCCGCGGCCCAGGCTCTCGGCGCGGCCCGGAGCGGCAGAAATCCTAAAATTCCATCAGAATGACACC TTCTCAGGTCACTTTTGAAATAAGAGGAACTCTTTTACCAGGAGAGGTCTTTGCAATATGTGGAAGCTGT GATGCCCTGGGAAACTGGAATCCTCAAAATGCTGTGGCTCTTATTAATGAAAACGAGACAGGAGACAGTG TGTTGTGGAAAGCAGTGATTGCTCTCAATAGAGGAGTGTCAGTGAAGTACCGCTACTTCAGAGGCTGCTT TTTAGAACCAAAGAAAGTGAAATCATTATTGACGATGGACAGTTTGGCATCCACAGGTAAAGCTCACACT CGAGGGTCTGGAGGAAGATGAAGATGATGATGACGATAAGGTCTCTCCCACTGTTCTTCACAAAATGTCC AACAGCCTGGAGATATCCTTAATAAGTGACAATGAGTTCAAGTGCAGGCACTCACAGCCAGAATGTGGGT ATGGCTTACAGCCCGATCGTTGGACAGAGTACAGCATACAGACAATGGAACCAGATAATCTGGAGCTCAT CTTTGACTTTTTTGAGGAAGATCTCAGTGAGCATGTAGTTCAGGGTGATGTTCTTCCTGGACACGTGGGC ACAGCATGCCTCCTGTCTTCTACCATTGCTGAGAGTGGAAGAAGCGCTGGAATCCTTACTCTTCCCATCA TGAGCAGAAATTCCAGAAAAACTATAGGCAAAGTCAGAGTTGATTTTATCATCATCAAGCCATTACCTGG ATATAGTTGTTCTATGCAGTCTTCATTTTCCAAGTATTGGAAACCAAGAATACCATTGGACGTTGGACAT CGTGGTGCAGGGAACTCAACAACGACTGCCAAGCTAGCTAAAGTACAGGAAAATACTATCGCTTCTTTAA GAAATGCTGCCAGTCATGGCGCAGCATTTGTAGAATTTGATGTCCACCTTTCAAAGGACTTTGTGCCCGT GGTGTATCATGACCTCACCTGCTGTCTGACCATGAAGAGGAAATATGAAGCTGATCCAGTTGAATTGTTT GAAATCCCAGTAAAAGAATTAACATTTGACCAACTCCAGTTATTGAAGCTTTCTCATGTGACTGCATTAA AAACCAAAGACCGGAAACAATCTTTGTATGAGGAGGAAAATTTCTTTTCTGAAAATCAGCCATTTCCTTC TCTTAAGATGGTTTTAGAATCATTGCCAGAAAATGTAGGATTTAATATAGAAATAAAATGGATTTGCCAA CACAGGGATGGAGTATGGGATGGCAACTTATCAACATATTTTGATATGAATGTGTTTTTGGATATAATTT TAAAAACTGTTTTAGAAAATTCTGGGAAGAGGAGAATAGTGTTTTCTTCTTTTGATGCAGATATTTGTAC AATGGTTCGGCAGAAGCAGAACAAATATCCCATATTATTTTTGACCCAAGGAAAGTCTGATATTTACCCC GAACTCATGGACCTCAGATCTCGGACAACACCCATTGCAATGAGTTTTGCACAGTTTGAAAATATTTTGG GGATAAATGCCCATACTGAAGACCTCCTTAGAAACCCATCCTATGTCCAAGAGGCAAAAGCTAAGGGATT GGTCATATTCTGCTGGGGTGATGATACCAACGATCCTGAAAACAGAAGGAAACTGAAGGAATTTGGAGTA AATGGTCTAATATATGATAGGATATATGATTGGATGCCTGAACAGCCAAATATATTCCAAGTGGAGCAGT TGGAGCGCCTGAAGCAAGAATTGCCAGAGCTTAAGAACTGTTTGTGTCCCACTGTTAGCCACTTCATCCC TTCTTCTTTCTGTGTGGAGCCTGATATCCACGTGGATGCCAACGGCATTGATAGTGTGGAGAACGCTTAG TTCACAGTGCACAGAGGACATTTAGGGGCACACCGCTGCCCTCATGTTTAGTTTTCACCATCGAGCACTG CTGTTGCCTCTTAGGTTTTTCAGTCCAATGAAGCAATAATGAAGTATTTTACTATTTCATTACAGGTCCC GCAAGAATATCAAGTATGCTATTTGTCACTTGTCCAGGTATAATTACCAATCAGTCTCTGTACAATAAGA AAATATACTGTTAAACATTTCAGTGAGATATCTAAGCCTATAATGGTGAATCTTCATTAAAAGTTGGAAA TTAACGATATAAATATCTCATACTTAAAGTTCAGGCTTTTCATTTGATTAAGTCTTAAATCTTCAGTGCT TGAGAAAAATGATTGCATAATTATACCTGACCATGGAAAAACTAAGTACCTCAATGCATGCATTTGCACT GGTGGCTCCAGCTGCACAGGTCTGTGTCATCCATGTACATAGGTATCTTTACATGGTTGATAGGAACACG CACCATTTTCCTTTAGTATAAACCTCAGATTGCTGCCATTTCTCACCTGACCCAAGCCAGCCTGCAGATG AACCTCAAAACTTGTCTCATAAACCATTCAAAGATATTAAAGTTCCAGAATGCTGCAGGGTAACTTAATG TATAAAGTATTTGTAAGAAGTATATATTGCATATATAGTAGTGTAGATCAGAAGGTATCAATTTGACTCA ATGCATGCTTTAGGTTTTAAGCATGAGATTGTACATGTTTACTGTTAAGTCCTTGCATCTGGTGCTAGGT GAGGGAGATGTTAAGGACATAAAATATTTTGTCGCCTAAAAAACAAAGTCTGTTTTAGACACTTTGAGTA TGCTTATTTAGTGTCTCACTACCTAGTAAACATAAGTGTCTCACTACCTAGTAAACATAATTGCTGTGTG TTGCATAGTAGTTAAATTCCCATGCAGAAAAATAAATGTCCTGAATTCTCAAATTGGTATTCTTTATTGT TTAATATATATCATGTATGTAATTTATTTAGGAATGTAGATCTTACTGTATATAAATGCATGCTTTTAGG ATTATACTAAGATTTCTTTAGAAGCAGATTGTACTGATAAAACTGTAACTTCAGAATGAATGTTAAATAA AATGACAGATTTATTTTTTTCCTCATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5357683 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5357683 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7306 same embryo
	EMAGE:7307 same embryo
	EMAGE:7308 same embryo
	EMAGE:7304 same embryo
	EMAGE:7305 same embryo
	EurExpress:euxassay_004349 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source