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EMAGE:731

Shh sonic hedgehog ( MGI:98297)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:731
Figure 6G of Filosa et al., 1997 [PMID:9226455] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:731EMAGE:731Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
731_voxel_strong_3D_1.wlz
731_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:731_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
neural tube floor plate
detected detected
foregut
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23359
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Filosa et al., 1997 [PMID:9226455] was previously described by Echelard et al., 1993 [PMID:7916661] as "either a 2.8 kb or 0.6 kb RNA transcript generated by T7 (2.8 kb) or T3 (0.6 kb) transcription of XbaI and HindIII digests of Bluescript (Stratagene) subclones of the original Shh cDNA inserts."
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:involves: 129/Sv x C57BL/6 x CD-1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:7.5% gelatin
General Information
Authors:Filosa et al., 1997 [PMID:9226455] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9226455] Filosa S, Rivera-Perez JA, Gomez AP, Gansmuller A, Sasaki H, Behringer RR, Ang SL 1997 Goosecoid and HNF-3beta genetically interact to regulate neural tube patterning during mouse embryogenesis. Development (124):2843-54
 [ doi:10.1016/0092-8674(93)90627-3] [ PMID:7916661] Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP 1993 Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell (75):1417-30
Links:MGI:1338649 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI