EMAGE mouse gene expression spatial database

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EMAGE:7315

Ruvbl1 RuvB-like protein 1 ( MGI:1928760)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004392_01	euxassay_004392_02	euxassay_004392_03	euxassay_004392_04	euxassay_004392_05

euxassay_004392_06	euxassay_004392_07	euxassay_004392_08	euxassay_004392_09	euxassay_004392_10

euxassay_004392_11	euxassay_004392_12	euxassay_004392_13	euxassay_004392_14	euxassay_004392_15

euxassay_004392_16	euxassay_004392_17	euxassay_004392_18	euxassay_004392_19	euxassay_004392_20

euxassay_004392_21	euxassay_004392_22	euxassay_004392_23	euxassay_004392_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7315_wholemount_strong.wlz

7315_wholemount_moderate.wlz

7315_wholemount_weak.wlz

7315_wholemount_possible.wlz

7315_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7315_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6400
Entity Detected:	Ruvbl1, RuvB-like protein 1 ( MGI:1928760)
Sequence:	sense strand is shown >T6400 GTAACCCAGGCGCCTGGCGTGCGGTAGTTCCTACCACTCCACGCAGGTTGTGTTCCTAGACACTCGGCAA AATGAAGATTGAGGAGGTGAAGAGCACCACGAAGACGCAGCGCATCGCCTCCCACAGCCACGTGAAGGGT CTGGGGCTGGACGAGAGCGGCCTAGCCAAGCAGGCGGCTTCGGGTCTCGTGGGCCAGGAGAACGCGAGAG AGGCATGTGGCGTCATTGTAGAATTAATCAAAAGCAAGAAAATGGCTGGAAGAGCTGTCTTGTTGGCGGG GCCTCCTGGAACTGGCAAGACAGCCTTGGCCCTTGCTATTGCTCAGGAGCTGGGCAGTAAAGTCCCTTTC TGCCCGATGGTGGGTAGCGAGGTATACTCAACTGAGATCAAAAAGACAGAGGTGCTGATGGAGAACTTCC GCAGGGCCATTGGGTTGCGGATAAAGGAGACTAAGGAGGTTTATGAAGGGGAGGTGACAGAGCTCACTCC CTGTGAGACAGAGAACCCCATGGGTGGGTACGGCAAAACTATCAGCCATGTGATCATAGGGCTCAAGACT GCTAAAGGAACCAAACAGCTGAAGCTGGACCCCAGTATTTTTGAAAGTTTGCAGAAAGAACGAGTAGAGG CTGGAGATGTGATTTACATTGAAGCAAATAGTGGAGCTGTGAAGAGGCAAGGCAGGTGTGACACCTATGC CACAGAGTTTGACCTTGAAGCTGAAGAGTACGTCCCTTTGCCAAAGGGAGATGTGCACAAGAAGAAGGAA ATCATACAGGATGTGACCTTGCATGACCTGGATGTGGCTAATGCGCGGCCTCAGGGTGGGCAAGATATTC TGTCTATGATGGGCCAGTTGATGAAGCCAAAAAAGACAGAGATCACAGATAAACTTCGAGGGGAGATCAA CAAAGTGGTGAACAAATACATTGACCAGGGTGTGGCCGAGCTGGTCCCTGGAGTGCTTTTTGTCGATGAG GTCCACATGCTGGATATCGAGTGCTTTACCTACCTGCACCGAGCCCTGGAGTCCTCCATCGCCCCCATTG TCATCTTTGCATCCAACCGAGGCAACTGTGTCATCAGGGGCACTGAGGACATCACTTCTCCACACGGCAT CCCGTTGGATCTGCTAGACAGGGTGATGATCATCAGGACCATGCTATACACGCCACAGGAGATGAAGCAG ATCATTAAGATCCGAGCCCAGACGGAAGGCATCAACATCAGCGAGGAGGCGCTGAACCACCTCGGGGAGA TTGGCACCAAGACCACTCTGAGGTATTCGGTGCAGCTGCTGACCCCAGCCAACCTGCTGGCAAAGATCAA TGGCAAGGACAGCATTGAGAAGGAGCACGTGGAAGAGATCAGCGAGCTCTTCTATGACGCCAAGTCCTCT GCCAAGATTTTGGCTGACCAGCAGGACAAGTACATGAAGTAACGTTGGGATGGAGGCGCACCCAGAGACG GACCCCACACCGTGCACAGGGGCTCGCCTCAAGCATGCTTTGCAGTGTTACAGTTTCTTTGTAAATTATC AAACCTCCATGGTGGTTTGGAAGGATCCCTTCCCCACCTAGCTTGTTTTCTAATAAAACTGAATAGGTTA TTTTGATGATAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3582426 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3582426 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7316 same embryo
	EMAGE:7317 same embryo
	EMAGE:7318 same embryo
	EMAGE:7314 same embryo
	EMAGE:7319 same embryo
	EurExpress:euxassay_004392 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source