EMAGE mouse gene expression spatial database

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EMAGE:7317

Btn1a1 butyrophilin, subfamily 1, member A1 ( MGI:103118)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004383. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004383_01	euxassay_004383_02	euxassay_004383_03	euxassay_004383_04

euxassay_004383_05	euxassay_004383_06	euxassay_004383_07	euxassay_004383_08	euxassay_004383_09

euxassay_004383_10	euxassay_004383_11	euxassay_004383_12	euxassay_004383_13	euxassay_004383_14

euxassay_004383_15	euxassay_004383_16	euxassay_004383_17	euxassay_004383_18	euxassay_004383_19

euxassay_004383_20	euxassay_004383_21	euxassay_004383_22	euxassay_004383_23	euxassay_004383_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7317_wholemount_strong.wlz

7317_wholemount_moderate.wlz

7317_wholemount_weak.wlz

7317_wholemount_possible.wlz

7317_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7317_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart valve

moderate

regional

moderate expression: see section 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6397
Entity Detected:	Btn1a1, butyrophilin, subfamily 1, member A1 ( MGI:103118)
Sequence:	sense strand is shown >T6397 CGGACGCGTGGGTCGAAATCGCCTGAAGCTCTTGGCGGGCTTCATTGCCCCAGTTAGCTCAGAGATGGCA GTTCCCACCAACTCCTGCCTCCTGGTCTGTCTGCTCACCCTCACTGTCCTACAGCTGCCCACGCTGGATT CGGCTCCCTTCGATGTGACCGCACCTCAGGAGCCAGTGTTGGCCCTAGTGGGCTCAGATGCCGAGCTGAC CTGTGGCTTTTCCCCAAACGCGAGCTCAGAATACATGGAGCTGCTGTGGTTTCGACAGACGAGGTCGACA GCGGTACTTCTATACCGGGATGGCCAGGAGCAGGAGGGCCAGCAGATGACGGAGTACCGCGGGAGGGCGA CGCTGGCGACAGCCGGGCTTCTAGACGGCCGCGCTACTCTGCTGATCCGAGATGTCAGGGTCTCAGACCA GGGGGAGTACCGGTGCCTTTTCAAAGACAACGACGACTTCGAGGAGGCCGCCGTATACCTCAAAGTGGCT GCTGTGGGTTCAGATCCTCAAATCAGTATGACGGTTCAAGAGAATGGAGAAATGGAGCTGGAGTGCACCT CCTCTGGATGGTACCCAGAGCCTCAGGTGCAGTGGAGAACAGGCAACAGAGAGATGCTACCATCCACGTC AGAGTCCAAGAAGCATAATGAGGAAGGCCTGTTCACTGTGGCAGTTTCAATGATGATCAGAGACAGCTCC ATAAAGAACATGTCCTGCTGCATCCAGAATATCCTCCTTGGCCAGGGGAAGGAAGTAGAGATCTCCTTAC CAGCTCCCTTCGTGCCAAGGCTGACTCCCTGGATAGTAGCTGTGGCTATCATCTTACTGGCCTTAGGATT TCTCACCATTGGGTCCATATTTTTCACTTGGAAACTATACAAGGAAAGATCCAGTCTGCGGAAGAAGGAA TTTGGCTCTAAAGAGAGACTTCTGGAAGAACTCAGATGCAAAAAGACTGTACTGCATGAAGTTGACGTGA CTCTGGATCCAGACACAGCCCACCCCCACCTCTTCCTGTATGAAGATTCAAAGTCAGTTCGATTGGAAGA TTCACGTCAGATCCTGCCTGATAGACCAGAGAGATTTGACTCCTGGCCCTGTGTGTTGGGCCGTGAGACC TTTACTTCAGGGAGACATTACTGGGAGGTGGAGGTGGGAGATAGAACTGACTGGGCCATTGGTGTGTGTA GGGAGAATGTGGTGAAGAAAGGGTTTGACCCCATGACTCCTGATAATGGGTTCTGGGCTGTGGAGTTGTA TGGAAATGGGTACTGGGCCCTCACCCCACTCAGGACCTCTCTCCGATTAGCAGGGCCCCCTCGCAGAGTT GGGGTTTTTCTGGACTATGACGCAGGAGACATTTCCTTCTACAACATGAGTAACGGATCTCTTATCTATA CTTTCCCTAGCATCTCTTTCTCTGGCCCCCTCCGTCCCTTCTTTTGTCTGTGGTCCTGTGGTAAAAAGCC CCTGACCATCTGTTCAACTGCCAATGGGCCTGAGAAAGTCACAGTCATTGCTAATGTCCAGGACGACATT CCCTTGTCCCCGCTGGGGGAAGGCTGTACTTCTGGAGACAAAGACACTCTCCATTCTAAACTGATCCCGT TCTCACCTAGCCAAGCGGCACCATAACAAATATTCCAGCTTCACGACTTTGCCTTCCTTTGACTAATCCC TCATGCCCCGAAGCTTCAGCTGTTGGCTTCTTGCAGCCCTGCTTCTTCCTGGTGGATGGAGATTAATTCA CATTGGGAAGGTTAGGTATGTTGCTGCCAGACAAGGCAGGAAGAAAGGCCATCCTAGTTTGTTTCTGTAC TAACAGTGGGGAGGAAGAGAGCTGAATCCTAAACTATTTCCAGTGCTCATATTCCTTCAGGCCAGAGCCT ATAGAGAAGGATTTGGTACAATCACTCGAGGGATCAAGAGGCAATTAGGTTGGCATGGAATTATGGCAGA AACATCTGGAATAGGGGTATGTGGAATGACAGGTTTTAGGTAAGGGAGAACAAAACCAAACCATAGGATG CTGAGAAAGAAAGATCTTGGACTAAACTCCTAAAAAAGCACTTAGAGAAGATATGACAGGCAAATGAAGT GAATTTGGTCTAATTTGATACACTTGCCCTGTCCCTAGGGTTTTTCAGTTATATCTCAATTTTTTTGTTG TTAATTACATTTTTGACAGCTTCATACATGTATATAATGCATTCTAATTACTCTCACTCTCCTCTATTCT GTCTTATTTCCCTCCCCTCCCCTCATACCTTCCTTCTTGCTTCAAACCTGGCACACTGAGTTTAATGGGC TATCATGGGAACATGGATTTAGAGCTTTCCTCTGAGCTCAAGAGAGCAGGTGTGACTGAATACAGTGATT TCCCCTCTCCTACAATCAATCAGCAGTCAATAGCTCAGCTGGGAGGGGTAGGGCCTCATGAGACTTCCCC TATCAAGGCTAAATGTTGAAAGGGCCAGTTTTTAGCACCTGTGAGATCATGATTGCAAGAGCCCAGAAGA CAGCATTGCTCGGTCATTCTCCCTACCCTTTGGCTTTTCTGGTCTTTTGTCCTCTCTTTCAGGATGTGTC TGAACTCTGTATCTTAAGTTTTCTATGTCATGTTCTATAAGATAGAGGAGACTGGCCCTGCTTGTTTGAG AGCAATGTGAGCAAGCTAGCAAGAGACAGAAAGGAGCGGAGATGAATAGGGGTAGAGAAAATTTTTAAAC AAACCCTCCAGGTGTGTGTGTGTGTGTGTGTGTCTTCCTCTTTTTTGACCTCCCTAAAGGTCAATCCAAC CTCACATTATTGACTCCACTAGGTGGGGGTTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTTTTAAGATAGAGGTTTACTATGTAGCTTAGGCTGGCTTTGAATTCCTGATCCTCCTGCCTCTACCTT CCAAGTGCTGGAAACATAGCCACATCCACCACCCCTATCCAGTCCACCTGGTTTGATTCAGCAACGCTCA GGTAGCATCGCTGTTTGATCTGGAGCTGCCAGCTCCCTCGGCCCCCACTGCAATGCTTAACCCCCTCACA GGCACCTTCCCTTGCCTAACACTGCCATCCTTTTCCACACTGAGCCATTTGCTCAATGTAGCCTACCCAG GTATCCTGCTTTCTGGTCCCCAAAGTTACACCATGATGCTCAGCACAGCTGGACAGTTTGTCCCAATTTG TGTGTGTCCTCCTGTTTGTATGGGACTTCTTTTTGTCAATGGCCTGTGTGTGTATCCAAGCTCTTCCACT TCTATTGTATTTTTCCGGCTTCTAAAACAGATGTTACCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3582574 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3582574 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7316 same embryo
	EMAGE:7318 same embryo
	EMAGE:7315 same embryo
	EMAGE:7314 same embryo
	EMAGE:7319 same embryo
	EurExpress:euxassay_004383 same experiment
	MGI:4823519 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source