EMAGE mouse gene expression spatial database

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EMAGE:7356

Rab28 RAB28, member RAS oncogene family ( MGI:1917285)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004499. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004499_01	euxassay_004499_02	euxassay_004499_03	euxassay_004499_04

euxassay_004499_05	euxassay_004499_06	euxassay_004499_07	euxassay_004499_08	euxassay_004499_09

euxassay_004499_10	euxassay_004499_11	euxassay_004499_12	euxassay_004499_13	euxassay_004499_14

euxassay_004499_15	euxassay_004499_16	euxassay_004499_17	euxassay_004499_18	euxassay_004499_19

euxassay_004499_20	euxassay_004499_21	euxassay_004499_22	euxassay_004499_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7356_wholemount_strong.wlz

7356_wholemount_moderate.wlz

7356_wholemount_weak.wlz

7356_wholemount_possible.wlz

7356_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7356_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb digit 3 mesenchyme

weak

regional

weak expression: see section 23

hindlimb digit 1 mesenchyme

strong

regional

strong expression: see section 05 06 moderate expression: see section 19 20 weak expression: see section 03 04 21

hindlimb digit 2 mesenchyme

strong

regional

strong expression: see section 05 06 moderate expression: see section 20 weak expression: see section 03 04 21

hindlimb digit 3 mesenchyme

strong

regional

strong expression: see section 05 06 moderate expression: see section 20 weak expression: see section 03 04 21 22

hindlimb digit 4 mesenchyme

strong

regional

strong expression: see section 05 06 moderate expression: see section 20 weak expression: see section 03 04 21

hindlimb digit 5 mesenchyme

strong

regional

strong expression: see section 05 06 moderate expression: see section 19 20 weak expression: see section 03 04 21

naris

moderate

regional

moderate expression: see section 08 11 12 13 weak expression: see section 09 10

viscerocranium

moderate

regional

Expression in the turbinate bone.

sternum

moderate

regional

moderate expression: see section 12 weak expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6472
Entity Detected:	Rab28, RAB28, member RAS oncogene family ( MGI:1917285)
Sequence:	sense strand is shown >T6472 GGGGAGGAGGGCAAGATGGCGGCCGGTGAGTGATTCTCCCTGGCTTCCTTCAGGGCCGAGGAGACGCCGG GGCGGGGGACCCGCTCGTGCAGCCCGCCGGCCTCCTCACCCCCGCCCCCGGAGGCCTCCTCTTTCCCGTC CTCCACCTCGGGGGCAGGCCAGCATCCTCGGACACCATGTCAGACTCTGAGGAGGAGAGCCAGGACCGGC AACTGAAAATCGTCGTGCTGGGGGACGGCACCTCGGGGAAGACCTCCTTAGCTACGTGTTTTGCTCAAGA GACATTTGGGAAGCAGTACAAGCAGACGATAGGACTGGATTTCTTTTTGAGAAGGATTACCTTACCAGGA AATTTGAATGTTACTCTTCAAGTTTGGGATATAGGAGGGCAGACAATAGGTGGCAAGATGTTGGATAAAT ACATCTATGGAGCACAGGGAATCCTCTTGGTATATGATATTACAAACTACCAAAGCTTTGAGAATTTGGA AGATTGGTATTCTGTGGTGAAGACAGTAAGTGAAGAGTCAGAAACTCAGCCCCTGGTTGCTTTAGTGGGC AATAAAATTGACTTGGAGCATATGCGAACAGTAAAAGCTGATAAGCACTTACGATTTTGCCAGGAAAATG GTTTTAGTAGCCACTTTGTTTCAGCCAAGACAGGAGACTCTGTCTTCCTGTGTTTTCAGAAAGTTGCAGC AGAAATCCTGGGAATCAAATTAAACAAAGCAGAAATAGAACAGTCACAGAGAGTGGTGAAGGCAGATATT GTAAACTACAACCAGGAGCCCTTGTCAAGAACTGTTAACCCTCCTCGGAGCTCCATGTGTGCAGTGCAGT GAGCACACACTGTATTGATAGTTCTGGCTGCTCTTCCCCTGAATTGGGCCCCAGCTGCCTAGGAACGTGT TTTATCAGTGACTGTCTCCGTAGCCAGCTCACACTTCCTCCTGCTCTGCTCCTTCATCCTTTAGTTGCCT GAGGGATCAGGCAGCTCCCTGGACCGGAAGGAGTGCGATTTGGAACCACACGTTTTGAAATCCGAATGGA GAGTCCATTCTCTGGATTGAGACTGCCTCCTCGCTCCAGAAGAACCATGTTGCTTCCTTCTCTCTTCACT TCTTTGTTTCCCAGATATAAAAAATTTAACCAGATATGAAGCTGCTCCAAGCTTTGATGATCATTTTCTA TCTGAACTTGTGTATATGAAAGTGTACTTCAGAGCGCTGATGGTGAAAGCCTCTCAAGAAGGTGTTCCTT TGCTGGCTATTGAGAGTACAATATACAAAGCTGTATCTAATTGAAAGTGCTTCAAATGAGGCTGTAATAG AAATATTTTTTCATTTTTAAAGGAGCCTAATGTGTTTATATTGTAGTTTATAACTCACAAGTAGTGAGTA TTTGATATTGTATTTTTATTACTGTATCATGGTCATTGCATATTTTATCATATTTGGCTTAGATGGCTTT ATGGATTTTGATAAATGGTTCAGCTGATCCTCCACATGGAATTTCTCCTACAGACTGTATGAGAGTGCTT CCAGATGAGTCATGATCTCCTTGGATCATTGGGGATCGATCACACTGGACCTGTTGTGCAGATGGTTTTC TCAGATAAATAAATTTATTTAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3587008 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3587008 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7353 same embryo
	EMAGE:7357 same embryo
	EMAGE:7355 same embryo
	EMAGE:7358 same embryo
	EMAGE:7354 same embryo
	EurExpress:euxassay_004499 same experiment
	MGI:4827572 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source