EMAGE mouse gene expression spatial database

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EMAGE:7357

Bub3 budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) ( MGI:1343463)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004484. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004484_01	euxassay_004484_02	euxassay_004484_03	euxassay_004484_04

euxassay_004484_05	euxassay_004484_06	euxassay_004484_07	euxassay_004484_08	euxassay_004484_09

euxassay_004484_10	euxassay_004484_11	euxassay_004484_12	euxassay_004484_13	euxassay_004484_14

euxassay_004484_15	euxassay_004484_16	euxassay_004484_17	euxassay_004484_18	euxassay_004484_19

euxassay_004484_20	euxassay_004484_21	euxassay_004484_22	euxassay_004484_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7357_wholemount_strong.wlz

7357_wholemount_moderate.wlz

7357_wholemount_weak.wlz

7357_wholemount_possible.wlz

7357_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7357_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 12 13 14 15 16

telencephalon mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 13

hindbrain

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15

midbrain lateral wall

moderate

regional

moderate expression: see section 09

midbrain mantle layer

moderate

regional

moderate expression: see section 08 10 11 12 13 14 15

saccule

strong

regional

strong expression: see section 01 02 03 04 05 19 20 21 22 23

utricle

strong

regional

strong expression: see section 01 02 03 04 05 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6469
Entity Detected:	Bub3, budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) ( MGI:1343463)
Sequence:	sense strand is shown >T6469 CGCTTTGCCTGCGAGTGCTCGGAGGGAAGCGGATCGGTAGTGGCCGGTGCAGAAACGTTGCTTAGGCGGA CAGGAGATGACCGGTTCGAACGAATTCAAGCTGAACCAGCCACCCGAGGATGGCATCTCCTCGGTTAAGT TCAGCCCCAACACATCCCAGTTCCTCCTGGTCTCCTCCTGGGACACGTCTGTGCGCCTCTACGATGTGCC CGCCAATTCCATGAGGCTCAAGTACCAGCACACTGGTGCTGTCCTGGACTGCGCCTTCTACGATCCAACG CATGCCTGGAGTGGTGGATTAGATCATCAACTGAAAATGCACGATTTGAACACTGATCAAGAAAATCTTG TTGGAACTCACGATGCCCCTATCAGATGTGTTGAATACTGTCCAGAAGTGAACGTGATGGTAACTGGAAG TTGGGATCAGACAGTTAAACTGTGGGACCCCAGAACTCCGTGTAATGCTGGGACTTTCTCCCAGCCGGAA AAGGTCTACACCCTGTCAGTGTCTGGGGACAGGCTGATTGTGGGCACGGCGGGCCGCCGAGTGCTGGTGT GGGACTTGCGGAACATGGGCTATGTGCAGCAGCGGAGGGAGTCCAGCCTGAAGTACCAGACTCGCTGCAT CCGAGCCTTCCCGAACAAGCAGGGTTATGTGTTGAGCTCCATCGAAGGCCGAGTGGCTGTGGAATACTTG GACCCGAGCCCTGAGGTGCAGAAGAAGAAGTACGCCTTCAAGTGCCACAGGCTAAAGGAGAACAACATTG AGCAGATTTACCCAGTCAACGCCATCTCCTTTCACAACATCCACAACACGTTTGCCACAGGTGGTTCGGA TGGATTCGTCAATATTTGGGACCCATTTAACAAGAAGCGCCTGTGCCAGTTCCATCGGTACCCCACCAGC ATCGCTTCCCTTGCCTTCAGTAATGACGGAACTACGCTTGCAATAGCATCGTCATATATGTATGAGATGG ATGACACCGAGCATCCTGAAGATGGTATCTTCATTCGCCAAGTGACAGACGCAGAAACAAAGCCCAAGTC CACGTAATCTGGGGAAAGCAGTTTCTCTGGAAAAGCTTTGCTTCCTACAAACACACGCTTTCCCTGAGGC CTGCTTGCAGCCATTGGACACTTGGTCTAGTGTGTGGATTTCTATGTCTTCTGTCGTAACAGAATTGTCT CTGTGCCCTGTGATTGTTTTGGGTAGGGACGCTTTGTATCCTTGTACAGTAGTCTTGACGATTCTCCTCC CCTACGCCATTTTTGTTTGTGTTTGCTATCTAAAACTGTTATATTTTTCTGTTGTCTAATAAATCAGAGA TTTGGAACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3586753 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3586753 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7353 same embryo
	EMAGE:7356 same embryo
	EMAGE:7355 same embryo
	EMAGE:7358 same embryo
	EMAGE:7354 same embryo
	EurExpress:euxassay_004484 same experiment
	MGI:4823523 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source