EMAGE mouse gene expression spatial database

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EMAGE:7358

Wdr47 WD repeat domain 47 ( MGI:2139593)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004509. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004509_01	euxassay_004509_02	euxassay_004509_03	euxassay_004509_04

euxassay_004509_05	euxassay_004509_06	euxassay_004509_07	euxassay_004509_08	euxassay_004509_09

euxassay_004509_10	euxassay_004509_11	euxassay_004509_12	euxassay_004509_13	euxassay_004509_14

euxassay_004509_15	euxassay_004509_16	euxassay_004509_17	euxassay_004509_18	euxassay_004509_19

euxassay_004509_20	euxassay_004509_21	euxassay_004509_22	euxassay_004509_23	euxassay_004509_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7358_wholemount_strong.wlz

7358_wholemount_moderate.wlz

7358_wholemount_weak.wlz

7358_wholemount_possible.wlz

7358_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7358_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 19 20 21 22 23 24 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

vibrissa

moderate

regional

moderate expression: see section 03 04 05 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 19

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 08 09 moderate expression: see section 07 10 12 13 14 15 16 17 18 19

not examined

regional

not examined expression: see section 01 02 22 23 24

neural retina

moderate

regional

moderate expression: see section 01 02 22 23 weak expression: see section 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6470
Entity Detected:	Wdr47, WD repeat domain 47 ( MGI:2139593)
Sequence:	sense strand is shown >T6470 GGGGCGGACGCGAGCAGGACCGGCTGACGAGCTGATGGATTGACGTGCGGGCTGCTTAGCCCAGACCGCC GCCCTCGCGCTCGCGCCTTAGACTGGAATTGGCTGTCTCGGGGCTCTGGCCGCCGGCCTCTCCGCCGCGT TTTCCTCCACCGCTTCTGCCGGCTCCGCCCGGAGCTCTCAGGCTAGCAATATGACTGCTGAAGAAACGGT GAATGTGAAAGAGGTTGAGATCATTAAGCTGATTTTGGACTTCTTGAATTCAAAGAAGCTTCACATTAGT ATGCTGGCGCTGGAGAAGGAAAGTGGAGTCATAAATGGCCTGTTTTCAGATGATATGCTCTTCCTGAGGC AGCTGATCCTGGATGGCCAATGGGATGAAGTTCTTCAGTTCATTCAGCCTCTAGAATGTATGGAGAAATT TGACAAAAAAAGGTTTCGTTATATTATCCTGAAGCAGAAGTTTCTAGAAGCTTTGTGTGTTAACAATGCA ATGTCTGCAGAAGATGAGCCCCAGCATTTGGAATTTACCATGCAGGAAGCGGTGCAGTGTCTGCATGCTC TGGAGGAGTACTGCCCTTCTAAAGACGATTACAGCAAGCTATGTCTGCTCCTCACCTTGCCTCGTTTGAC CAATCACGCTGAGTTTAAGGACTGGAATCCCAGCACTGCACGAGTTCACTGCTTTGAAGAGGTCTGTGTC ATGGTTGCAGAGTTCATTCCTGCTGACAGGAAGCTAAGTGAGGCTGGTTTTAAAGCAAGTAACAATCGCT TATTCCAGCTCGTAATGAAGGGCCTACTTTATGAATGCTGCGTAGAATTTTGTCAGAGTAAAGCAACTGG TGAAGAAATTACAGAAAGTGAAGTCCTTCTTGGCATCGACCTGTTATGTGGCAACGGTTGTGACGATTTA GATCTAAGTTTATTGTCTTGGCTTCAGAATCTCCCTTCTTCAGTCTTTTCTTGTGCATTTGAACAGAAAA TGCTTAATATTCATGTTGACAAACTTCTGAAGCCTACAAAAGCTGCATATGCTGATCTGTTGACTCCTCT TATCAGCAAACTGTCTCCCTACCCTTCATCTCCAATGAGAAGACCTCAGTCAGCTGATGCCTATATGACT CGCTCTCTCAATCCTGCTTTGGATGGCCTCACATGTGGGTTAACCAGTCATGATAAGAGGATCTCAGATC TTGGGAACAAAACTTCTCCAATGTCACACTCCTTTGCTAACTTCCACTATCCAGGGGTACAGAACCTCAG CAGAAGTCTCATGCTTGAGAATACAGAGTGTCACAGCATCTATGAAGAATCTCCTGAGCGAAGTGATACG CCTGTTGAGGCCCAGCAGCCTGTGAGCAGTGAAGCCATGTGCCAGGGGTCAGGGTTAGAAAAAGAGCCTG CAAATGGAGCACAGAACCCAGTTCCAGCTAAACAAGAAAAAAATGAGCTTCGAGATTCCACTGAACAATT TCAGGAATATTACAGGCAAAGACTGCGCTACCAGCAGCACCTAGAACAGAAGGAGCAACAGCGGCAGATA TACCAGCAGATGTTACTTGAAGGAGGCGTGAACCAAGAGGATGGTCCTGATCAGCAGCAGAATCTTACTG AACAATTCCTTAATAGGTCTATTCAGAAGCTTGGCGAATTAAATATTGGGATGGATAGTCTTGGTAATGA GGTGCCAGTGCTCAACCAGCAATGCAGCGGGAGCAAAAACAATGGATCAAATAATTCCTCTGTGACTAGT TTCAGTACACCACCCCAAGATTCCAGTCAGAGGTTAATACATGATACTGCCAATATTCACACAAGCACTC CTCGTAATCCTGGATCAACGAATCACATCCCTTTTCATGAAGACTCACCTTGTGGAAGCCAGAACTCATC AGAACATTCTGTCATTAAGCCATCACCTGGAGATTCTTCAGGGAATCTATCAAGGTCAAAAGGGGAAGAG GATGACAAGTCAAAAAAGCAGTTTGTTTGTATTAATACCCTAGAAGACACACAGGCTGTTAGAGCCGTGG CATTTCATCCAAGTGGTAGTTTATATGCTGTTGGTTCAAATTCAAAAACTCTGAGAGTGTGTGCCTATCC AGAAAAAATGGATGCAAGTGCACACGACAATCCTAAGCAGCCAGTGGTGCGATTTAAAAGAAACAAACAT CATAAAGGATCTATTTATTGTGTGGCTTGGAGTCCGTGTGGACAGTTGTTAGCAACAGGATCAAATGACA AATATGTAAAAGTGCTGCCCTTCAATGCAGAGACTTGTAATGCAACAGGACCAGATCTGGAATTTAGCAT GCATGATGGGACAATTCGAGATTTGGCATTTATGGAAGGCCCAGAAAGTGGTGGAGCTATTTTAATAAGT GCTGGAGCAGGAGATTGTAACATTTATACAACAGACTGTCAAAGAGGACAGGGTCTTCATGCTTTGAGTG GACACACTGGGCACATTTTAGCACTTTATACCTGGAGTGGGTGGATGATTGCGTCTGGTTCCCAGGATAA GACTGTTAGATTCTGGGATCTTCGTGTACCAAGCTGTGTTCGTGTGGTTGGCACAACATTCCATGGAACT GGCAGTGCAGTGGCATCTGTGGCTGTGGACCCCAGTGGCCGTCTCTTAGCTACAGGTCAGGAGGACTCTA GCTGCATGCTGTACGACATAAGAGGAGGCAGAATGGTCCAGAGTTACCACCCTCACTCCAGTGACGTTCG CTCTGTTCGCTTCTCTCCTGGAGCTCACTATCTGCTGACAGGCTCTTACGATATGAAAATAAAGGTGACA GACCTGCAAGGGGACCTCACCAAGCAGCTTCCTCTCATGGTGGTGGGGGAGCACAAGGACAAGGTGATCC AGTGCAGATGGCACACCCAGGATCTCTCCTTCCTCTCATCCTCTGCAGACAGAACTGTGACCCTCTGGAC TTACAGTGGCTAGAGGCCACCCTGCACTGGCTCTGCGGTGCAAGCACAGACCCCAGACTACTGAACTGTT AGGACTACAGGCCTGAAGAACGCGTCCTGCCCCAGAAAGAGCTTAAGTGGAGGAAGCCCCCTTATTACCA TTTCCTGTGAACAGAAGGTGTTGGGTGGTGGTTTTCAGTCTTCCATGTGTGTTCATGCTGCTGTGTAGTT TCCTTGCCAAAGTCTGCAGAAGAGCTCTTATGTTGTTGGTTTGCACCCCAAGTCATGGTCCAGGATGGGT TCATGGTTATTAAGTGCACTCCTTGGGTGTTACCCAGGTCACAGTTTATACTCACACTGACATGGAATTA TAATTTAAGTAATTGTTACACATACTAGATCCAGGTTCTCCCCCAGCTAAAACAGAGGTATTCAGAAATG ACTTTGCTCTTGTCACAATCCCATACTGTATCACGTGTCTTCTAGAGGTCAGGGTTTGGTAAGTCTCCAA TTTTACCTGATCATCTTTGCATAGGGCTCACCACAGAGCCTCCAGATGAAAGGAGAGCTGCTTCTTCCTC TTAACCCAGGGGTGTGGTTGCTAATTTGACACGTCACTTTGTTTCAGGCTAAAAGGTAATAACCATTTTG CTTTGGGAACTTGTTGACTTCGGTTTTTTGAATGCACAAGGTCCCCCCTCCCTTCCAAACAGCACTGTAG AAGCAGAGCGGCAGCTGTTACGTGGAACAATAGTCAATGGTAGGTTAACTTACCAGGTAGCATTTTCCTC TTCAAAATTAAGACTTTGCCATGACCCTAGCATGGCTTGAAAAGCTATGTTTGCATGATAATTTAAAATG GAAACTGAGACTTTGCACTCCAGAAGCTTATTCGGATTTTCTTGCACAGTTTTGTGTAATGAGGAATAAT GATTTTATTTGTACAGTACTATATATCCTAACATTTTATGTATCTTTATTTTCATATTGTTCAGTATTTT ACTTTAAGTTATTAAATAGGCTACTTTATTTATTTCAGCTGCAGTGGTATTGACCTGTCTGTGCACTATA TGTAGCAAGCATTTTACATCTATAGTACACAAGTGGGAGGTAATAGAAGTGTAACTGTGCTTTTTATTTC AATAAAGACCTCTTGACACTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3586908 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3586908 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7353 same embryo
	EMAGE:7357 same embryo
	EMAGE:7356 same embryo
	EMAGE:7355 same embryo
	EMAGE:7354 same embryo
	EurExpress:euxassay_004509 same experiment
	MGI:4829202 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source