EMAGE mouse gene expression spatial database

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EMAGE:736

Myf5 myogenic factor 5 ( MGI:97252)
TS15 (25 Somite no.)
in situ hybridisation

Data Images


Fig 1 (centre) Summerbell et al, 2000 [PMID:10934019] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.	Fig 1 Summerbell et al, 2000 [PMID:10934019] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Authors state that the apparent staining in the head is possibly due to reagent trapping. The wholemount stained embryo was sectioned using a vibratome to produce the sections shown. Image annotations: psm, presomitic mesoderm; I, II, IV, VI, XIV and XV indicate somites 29, 28, 26, 24, 16 and 15 respectively; m, myotome; dm, dermomyotome; sb, somitic bud; td, thoracic diaphragm. Black arrows indicate expression in dorsal myotome, red arrows indicate Myf5 expressing cells that are not obviously located in either the epaxial or hypaxial domains, arrowhead indicates cells from ventral myotome that will migrate into the limbs that do not express Myf5.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

736_wholemount_strong_3D_1.wlz

736_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:736_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

tail unsegmented mesenchyme

not detected

Expression is absent in the presomitic mesoderm

trunk somite

strong

regional

- Expression is detected in the dorsalmost dermomyotome. Expression is detected in the dorsal myotome (Fig 1.I) - Expression is strong in the dorsal myotome and weak in the ventral myotome (Fig 1.II). - Expression is strong in the ventral myotome and the somitic bud (Figs 1.IV, VI) - Expression is detected in the ventral myotome and hypaxial cells that will migrate to the thoracic diaphragm (Fig 1.XIV) - Expression is absent in the ventral myotome cells that will migrate to the limbs (Fig 1.XV)

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1861714
Entity Detected:	Myf5, myogenic factor 5 ( MGI:97252)
Notes:	The probe used in this study by Summerbell et al, 2000 [PMID:10934019] is defined as follows: "Riboprobes were prepared using T7 RNA polymerase on an XbaI linearised 1063 bp (BbrPI/MscI) genomic subclone of Myf5. Only 354 bp of the 504 bp riboprobe form duplexes with Myf5 mRNA".
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	25 Somite no.
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	Mirsky's fixative
Staining procedure:	undefined

General Information

Authors:	Summerbell et al, 2000 [PMID:10934019] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:10934019] Summerbell D, Ashby PR, Coutelle O, Cox D, Yee S, Rigby PW 2000 The expression of Myf5 in the developing mouse embryo is controlled by discrete and dispersed enhancers specific for particular populations of skeletal muscle precursors. Development (127):3745-57
Links:	MGI:1888754 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source