EMAGE mouse gene expression spatial database

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EMAGE:7367

Nuf2 NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) ( MGI:1914227)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004486. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004486_01	euxassay_004486_02	euxassay_004486_03	euxassay_004486_04

euxassay_004486_05	euxassay_004486_06	euxassay_004486_07	euxassay_004486_08	euxassay_004486_09

euxassay_004486_10	euxassay_004486_11	euxassay_004486_12	euxassay_004486_13	euxassay_004486_14

euxassay_004486_15	euxassay_004486_16	euxassay_004486_17	euxassay_004486_18	euxassay_004486_19

euxassay_004486_20	euxassay_004486_21	euxassay_004486_22	euxassay_004486_23	euxassay_004486_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7367_wholemount_strong.wlz

7367_wholemount_moderate.wlz

7367_wholemount_weak.wlz

7367_wholemount_possible.wlz

7367_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7367_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6477
Entity Detected:	Nuf2, NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) ( MGI:1914227)
Sequence:	sense strand is shown >T6477 AAAATGACAACGGTCAGCGGCTTTCGGTTGGACGCTCGGTTTTTAACTGTTTTTCCGTGAGCTGCTAGGC CTCCAGGAGGACCTTGTTGAGCCTTTCCTGCGTCTACAGAGGTCTGTGGTTTCTTGAAACACAGGAAGCA ACAATACTCAGGGAGGAGACTCCCATAATGGAAACTTTGTCATTCCCCAGATACAATGTAGCTGAGCTTG TGGTTCATATTCGCAATAAATTATTAACAGGAGCAGATGGCAAAAACCTCTCGAAGAGCGATTTTCTTCC AAATCCGAAGTCTGATGTCTTGTACATGATTTACATGAAAGCCTTACAGTTAGTGTATGGAGTCCGGCTG GAGCACTTCTACATGATGCCGATGAACATAGAAGTCACATATCCACATTTAATGGAGGGCTTCTTACCAG TCAGAAGTTTGTTCTTTTACATGGATTCCTTTATGCCCATTTGCCGGGTGAATGACTTTGAGATTGTCGA CATTCTTAATCCAAGAACAAACCGGACAAGTCGTTTTTTAAGTGGCATTATCAACTTTATTCACTTCAGA GAAACGTGCCTGGAGAAGTGTGAAGAATTTCTTTTGCAAAATAAATCCTCTATGGTCAGAATGCAGCAGT TAAGCAATGTGCACCAGGAAGCTTTGATGAAACTGGAAAAACTCAATACCGTTCCGGCTGAGGAGCGAGA GGAGTTCAAGCAGTTTATGGATGACATTCAGGAGCTGCAGCACCTGCTGAATGAAGAGTTCAGACAGAAA ACGACACTGCTGCAGGAGGAATATGCCAAAATGAAATCCGATATTTCAGAGAAAACCAAGCATTTAAATG AACAAAAGTTGTCATTAGTTTCTTTGAAAGAAGTTGAAGACAATTTGAAAAGCAAAATTGTGGACTCTCC AGAGAAGCTGAAGAACTATAAAGACAAGATGAAAGGCACCGTCCAGAAGCTCCGCAGTGCCAGGGAAAAA GTGATGGAGCAGTATGATATCTATAGAGATTCTGTGGATTGCTTGCCTTCCTGTCAGCTGGAGGTGCAGT TGTATCAAAAGAAATCACAGGACCTTGCAGATAATAGGGAGAAACTAAGCAGTCTCTTAAAGGAGAGCTT GAACTTGGAGGACCAGATTGAAAGTGATTCATCGGAACTAAAGAAACTGAAGACTGAAGAAAATTCTCTC ATAAGAATGACGACTGTAAAGAAGGAGAAGCTCGCCACCGCGCGATTCAAAATCAACAAGAAGCAGGAGG ATGTGAAACATTACAAGCAGGCCATGATTGAAGACTGCAATAAAGTTCAAGAAAAGAGAGATGCTGTCTG TGAGCAAGTAACCACTGTTAATCAAGAAATCCACAAGATTAAATCTGCAATTCAACAACTGAGAGATACC AAGAAAAGGGAGATATTGAAGTCTCAGGAGATCTTTGTAAACCTGAAAAGTGCTCTGGAGAAGTACCATG AGGGCATCGAGAAGGTAGCGGAGGAGCGCAGCGCTAAACTGGAGGAGAAGACGGCAGAGCTGAAGAAGAG GATGGTCAGAATGGTCTGAACAACAACAGCCCTTCTGGAAACGGCCTGCTGCCTTTCTGCTTCTGTTTGG CATAGTTATATTGAAGCTAATGGAAGTACCTGCAATATCAGCTCACTAACACGGGTACCACTGGTTCTTG TTTCTCTTTGGTGTCATTTTATATGGCGATGTGCTTCCTGTGTGCTTCCAGACCTATCCCTGAGTTGAAA CTATTCCAATGGCCATTCATATTAGTTCTATTACAAGGCTGGAAGAGCTTAGATCCTTTCTACCCAGGCC CAACGACTACAGCTGATACAGCTGACATAAACTCTGATGGAGATACAACTCAGGGCCTTGCATGCTACAC TGACTTGCTTTCCCAGGCTCCAACATGCCATGTATTGTATAGACTTTTACTACAATTCAAATAACGTGTA CAGCTTTTATGTCCCTACTCTCTCTCTTTTTCTATGTACTGGTTGGAATAAATAGATACTGAACTCTGTG AGTTTTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3587655 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3587655 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7368 same embryo
	EMAGE:7369 same embryo
	EMAGE:7365 same embryo
	EMAGE:7366 same embryo
	EurExpress:euxassay_004486 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source