EMAGE mouse gene expression spatial database

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EMAGE:7376

Scpep1 serine carboxypeptidase 1 ( MGI:1921867)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004501. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004501_01	euxassay_004501_02	euxassay_004501_03	euxassay_004501_04

euxassay_004501_05	euxassay_004501_06	euxassay_004501_07	euxassay_004501_08	euxassay_004501_09

euxassay_004501_10	euxassay_004501_11	euxassay_004501_12	euxassay_004501_13	euxassay_004501_14

euxassay_004501_15	euxassay_004501_16	euxassay_004501_17	euxassay_004501_18	euxassay_004501_19

euxassay_004501_20	euxassay_004501_21	euxassay_004501_22	euxassay_004501_23	euxassay_004501_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7376_wholemount_strong.wlz

7376_wholemount_moderate.wlz

7376_wholemount_weak.wlz

7376_wholemount_possible.wlz

7376_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7376_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5747
Entity Detected:	Scpep1, serine carboxypeptidase 1 ( MGI:1921867)
Sequence:	sense strand is shown >T5747 CACGGCGTCACGCGATGGCCTTTGGCTCCTGCCTGTTGCTGATGTTGCGGCGGGGTTTTCATCATGGAGC TCTCGCGGCGGATCTGTCTCGTGCAACTGTGGCTGCTGCTCCTATCGTTCTTACTGGGCTTCAGCGCGGG ATCTGCCATCCACTGGCGGGAACCCGAAGGCAAGGAAGTATGGGATTATGTGACTGTCCGAAAGGATGCC CACATGTTCTGGTGGCTCTATTATGCCACCAACCCTTGCAAGAACTTTTCAGAGCTGCCCCTGGTCATGT GGCTTCAGGGTGGTCCGGGTGGTTCTAGCACTGGATTTGGAAACTTTGAGGAAATTGGCCCTCTTGACAC CCAACTCAAGCCTCGAAATACCACCTGGCTGCAGTGGGCCAGTCTCCTGTTTGTGGATAATCCCGTGGGC ACGGGCTTCAGCTACGTCAACACAACAGATGCCTACGCAAAGGACCTGGACACGGTGGCTTCCGACATGA TGGTTCTCCTGAAATCCTTCTTTGATTGCCATAAAGAATTCCAGACGGTTCCATTCTACATTTTCTCAGA ATCCTACGGAGGAAAGATGGCTGCTGGCATCAGTGTAGAACTTTACAAGGCTGTTCAGCAAGGGACCATT AAGTGCAACTTTTCTGGGGTTGCTTTGGGTGACTCCTGGATCTCCCCCGTGGATTCAGTGCTGTCCTGGG GACCTTACCTGTATAGTATGTCTCTCCTTGATAATCAAGGCTTGGCCGAGGTGTCCGACATTGCAGAGCA AGTCCTCGATGCTGTAAACAAGGGCTTCTACAAGGAGGCCACTCAGCTGTGGGGGAAAGCAGAAATGATC ATTGAAAAGAACACCGACGGGGTAAACTTCTATAACATCTTAACTAAAAGCAGCCCGGAGAAAGCTATGG AATCGAGCCTCGAGTTCCTTCGGAGCCCCTTAGTTCGTCTCTGTCAGCGCCACGTGAGACACCTGCAAGG AGACGCCTTAAGTCAACTCATGAACGGCCCCATCAAAAAGAAGCTCAAAATTATCCCTGAGGATATCTCC TGGGGAGCCCAGGCATCTTACGTCTTCCTAAGCATGGAAGGGGACTTCATGAAGCCTGCCATCGACGTTG TGGATAAGTTGCTGGCAGCTGGGGTCAATGTGACCGTGTACAACGGACAGCTGGATCTCATTGTGGACAC CATAGGTCAGGAGTCCTGGGTTCAGAAGCTCAAGTGGCCACAGCTGTCCAAATTCAATCAGCTAAAATGG AAGGCCCTGTACACCGATCCCAAGTCTTCAGAAACAGCTGCGTTCGTCAAGTCCTATGAGAACCTAGCCT TCTACTGGATCCTAAAGGCCGGTCACATGGTTCCTTCTGACCAAGGGGAGATGGCCCTGAAGATGATGAA GCTGGTGACCAAGCAGGAGTAGCTGAGCTGGCTGGCCCTGGAGGCGCTAAGAGCAGAGCCCAGGATGCAG GTGCTAATGTCTATCCCTGGTGCTCTTCTCCCCCGCTCTGCCATGGGATATGACTCTGGGAGCACCTGCT CTCTCCCGTACCAAAAGACTGTGGCCTTCTGTGTCTACTTAGAAATCAGTTCTGCTTCCCAAAGAGTATT TAAAACCAGACTCATTTAATCAAAGTGAAGGGTTGCAATCGATTGGTCTCTTACTACAAAAGCAGTTGAT AGCACATGTAAATCCCAGCACTTGAGAGGTAGAAGAAGCAAGAGGATGGATGAGAGGTTCAAACGCATCC GCAGCTACGTCGAAAGTTCAAAAGCAGCCTATGCCAAACAGGGAGAGTCCCTGTCCCCCACCCCCACCCC CAAAAAAGAGCAAAAGCAAACCACATGATTTATAGGCAAAAGCAGTGGGAGAGACAAAGAAAATACTTAA ACACCCACTGAGCTGCCAACTAGGTGTCTCTGACTACGGGTCTTTTATTTGCTACATATATATTTTTACA AAAATGAAATCCATATTGTACGCTATTTTGCTGTCTGCTTCGTTCCCATGTCAACATGACCTGCACTTCT TTTCCCATCAATAAATGTGTTGTGATATTTTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2650587 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2650587 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7381 same embryo
	EMAGE:7377 same embryo
	EMAGE:7380 same embryo
	EMAGE:7379 same embryo
	EMAGE:7378 same embryo
	EurExpress:euxassay_004501 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source