EMAGE mouse gene expression spatial database

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EMAGE:7382

Col1a2 collagen, type I, alpha 2 ( MGI:88468)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004456. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004456_01	euxassay_004456_02	euxassay_004456_03	euxassay_004456_04

euxassay_004456_05	euxassay_004456_06	euxassay_004456_07	euxassay_004456_08	euxassay_004456_09

euxassay_004456_10	euxassay_004456_11	euxassay_004456_12	euxassay_004456_13	euxassay_004456_14

euxassay_004456_15	euxassay_004456_16	euxassay_004456_17	euxassay_004456_18	euxassay_004456_19

euxassay_004456_20	euxassay_004456_21	euxassay_004456_22	euxassay_004456_23	euxassay_004456_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:7382

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7382_wholemount_strong.wlz

7382_wholemount_moderate.wlz

7382_wholemount_weak.wlz

7382_wholemount_possible.wlz

7382_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7382_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 01 02 03 04 05

limb

moderate

regional

moderate expression: see section 01 02 03 04 05

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05

gland

moderate

regional

moderate expression: see section 01 02 03 04 05

alimentary system

moderate

regional

moderate expression: see section 01 02 03 04 05

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05

cardiovascular system

moderate

regional

moderate expression: see section 01 02 03 04 05

urinary system

moderate

regional

moderate expression: see section 01 02 03 04 05

reproductive system

moderate

regional

moderate expression: see section 01 02 03 04 05

respiratory system

moderate

regional

moderate expression: see section 01 02 03 04 05

skeleton

moderate

regional

moderate expression: see section 01 02 03 04 05

tail

moderate

regional

moderate expression: see section 01 02 03 04 05

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6457
Entity Detected:	Col1a2, collagen, type I, alpha 2 ( MGI:88468)
Sequence:	sense strand is shown >T6457 CGGACGCGTGGGGTTTCGACTAAGTTGGAGGGAACGGTCCACGATTGCATGCCTGCGCCCGCCAGGTGAT ACCTCCGCTGGTGACCCAGGGGCTCTGCAACACAAGGAGTCTGCATGTCTAAGTGGTAGACATGCTCAGC TTTGTGGATACGCGGACTCTGTTGCTGCTTGCAGTAACTTCGTGCCTAGCAACATGCCAATATTTGCAAT CGGGATCAGTACGAAAGGGCCCCACTGGAGACAGAGGACCACGTGGACAAAGGGGTCCCGCAGGTCCCCG AGGCAGAGATGGTGTTGATGGTCCCATGGGCCCTCCTGGTCCCCCTGGCTCCCCTGGTCCTCCCGGCTCC CCTGCCCCCCCTGGTCTTACTGGGAACTTTGCTGCTCAGTATTCTGACAAAGGAGTTTCATCTGGCCCTG GACCAATGGGTTTAATGGGACCCAGAGGCCCTCCTGGTGCTGTTGGAGCCCCTGGCCCTCAAGGTTTCCA AGGACCTGCTGGTGAACCTGGTGAGCCTGGTCAAACGGGTCCTGCAGGTCCCCGTGGTCCAGCTGGCTCT CCTGGCAAGGCTGGTGAGGATGGTCACCCTGGAAAACCCGGAAGACCTGGGGAGAGAGGAGTCGTTGGAC CACAGGGTGCTCGTGGTTTCCCCGGGACTCCTGGACTTCCTGGCTTCAAAGGCGTGAAAGGACACAGTGG TATGGATGGATTAAAGGGACAGCCTGGTGCACAGGGTGTGAAGGGTGAACCCGGTGCCCCTGGAGAGAAT GGAACTCCAGGTCAAGCAGGAGCCCGAGGGCTTCCTGGTGAGAGAGGGCGTGTCGGAGCTCCTGGTCCAG CTGGTGCCCGAGGCAGTGATGGAAGCGTTGGCCCCGTCGGCCCTGCTGGTCCCATTGGGTCTGCTGGACC CCCTGGTTTCCCAGGTGCTCCTGGTCCCAAGGGTGAACTTGGGCCCGTTGGTAACCCTGGCCCTGCTGGT CCTGCTGGTCCCCGTGGTGAAGTGGGTCTTCCAGGTCTTTCTGGCCCTGTTGGACCTCCTGGCAACCCTG GAACAAATGGGCTCACTGGCGCCAAGGGTGCTACTGGACTCCCTGGTGTCGCTGGGGCTCCCGGTCTCCC TGGCCCCCGTGGTATTCCTGGCCCCGCTGGTGCTGCTGGTGCTACAGGTGCCAGAGGACTTGTTGGTGAG CCTGGTCCTGCTGGCTCCAAAGGAGAATCCGGTAACAAGGGTGAGCCTGGCTCTGTTGGAGCCCAAGGTC CTCCTGGTCCCAGCGGTGAAGAAGGAAAGAGAGGGTCTCCCGGAGAAGCTGGATCTGCTGGCCCTGCAGG GCCTCCAGGGCTTAGAGGCAGCCCTGGTTCTCGAGGTCTTCCTGGAGCTGATGGCAGAGCTGGTGTAATG GGTCCTCCTGGCAATCGTGGTTCAACCGGCCCTGCTGGAATCCGAGGTCCTAATGGAGATGCCGGTCGAC CTGGGGAACCTGGTCTCATGGGACCCAGAGGTCTTCCTGGGTCTCCTGGAAATGTTGGCCCATCTGGTAA AGAAGGCCCTGTGGGTCTCCCTGGCATTGATGGCAGACCTGGCCCAATCGGCCCAGCTGGACCAAGAGGT GAAGCTGGCAACATTGGATTCCCTGGACCCAAAGGCCCCTCTGGTGATCCTGGCAAACCTGGTGAGAGAG GACACCCCGGTCTTGCTGGTGCTCGGGGAGCTCCAGGACCCGATGGCAACAATGGAGCTCAGGGCCCCCC TGGACCCCAGGGTGTTCAAGGTGGCAAAGGTGAACAAGGCCCTGCTGGTCCTCCTGGCTTCCAGGGTCTC CCTGGTCCCTCAGGTACTACTGGAGAAGTTGGCAAGCCCGGAGAAAGGGGTCTTCCTGGTGAATTCGGTC TCCCTGGTCCTGCTGGTCCAAGAGGAGAACGTGGTACCCCGGGTGAGAGTGGAGCTGCTGGCCCTTCTGG TCCTATTGGAAGCCGAGGTCCCAGTGGAGCCCCAGGGCCTGATGGAAACAAGGGTGAAGCTGGTGCAGTC GGTGCTCCAGGCAGTGCTGGTGCCTCTGGGCCTGGTGGGCTTCCAGGAGAGAGGGGTGCTGCTGGCATAC CTGGGGGCAAAGGAGAAAAGGGTGAAACTGGTCTCCGAGGTGACACTGGCAACACTGGTAGAGATGGTGC TCGTGGCATTCCTGGTGCTGTAGGTGCCCCTGGTCCTGCTGGGGCCTCAGGTGACCGGGGTGAAGCTGGT GCTGCCGGTCCTTCTGGCCCAGCTGGTCCTCGGGGTAGCCCTGGTGAACGTGGTGAAGTTGGCCCTGCTG GCCCCAATGGATTTGCTGGTCCTGCTGGTGCTGCTGGCCAACCCGGTGCTAAAGGAGAGAAGGGAACCAA AGGGCCTAAGGGTGAAAATGGCATTGTTGGTCCAACCGGGTCTGTTGGAGCTGCTGGCCCATCTGGTCCT AATGGCCCACCTGGTCCTGTTGGAAGTCGTGGTGATGGAGGGCCCCCTGGTATGACTGGCTTCCCTGGTG CTGCTGGACGGACTGGTCCCCCCGGACCCAGTGGTATTGCTGGCCCCCCTGGCCCCCCTGGTGCTGCTGG AAAGGAAGGGATTCGTGGTCCCCGTGGCGACCAAGGTCCAGTTGGCCGAACTGGAGAAACAGGTGCATCT GGACCCCCTGGATTTGTTGGTGAAAAGGGTCCCTCTGGAGAACCTGGTACCGCAGGAGCCCCTGGTACCG CAGGTCCTCAGGGTCTTCTTGGTGCTCCCGGTATTCTGGGTCTCCCTGGCTCTAGAGGTGAACGTGGTCT GCCAGGTATTGCTGGTGCTCTGGGTGAACCTGGTCCTCTGGGCATCTCAGGCCCTCCTGGTGCCCGTGGT CCCCCTGGTGCTGTGGGCAGCCCTGGAGTTAATGGTGCCCCTGGTGAAGCTGGTCGTGATGGTAACCCTG GCAGCGATGGTCCCCCAGGTCGTGATGGTCAGCCTGGACACAAGGGAGAGCGTGGTTACCCTGGCAGCAT TGGTCCGACTGGTGCTGCAGGTGCCCCTGGTCCTCACGGTTCTGTGGGTCCTGCTGGGAAACATGGAAAC CGAGGTGAACCTGGTCCTGCCGGTTCTGTTGGTCCTGTTGGTGCTGTTGGTCCAAGAGGTCCTAGTGGTC CTCAAGGCATCCGAGGTGACAAAGGAGAACCTGGTGATAAAGGGCACAGGGGTCTTCCTGGCTTAAAAGG ATACAGTGGATTGCAGGGTCTTCCTGGTCTTGCTGGCCTACATGGTGACCAAGGAGCTCCTGGTCCTGTG GGTCCTGCTGGTCCCAGGGGTCCTGCTGGGCCTTCTGGACCCGTTGGCAAAGATGGTAGATCTGGGCAAC CTGGCCCAGTCGGGCCTGCTGGTGTTCGTGGTTCTCAGGGTAGCCAAGGTCCCGCTGGTCCCCCTGGTCC CCCTGGACCTCCTGGTCCTCCTGGTGTCAGCGGAGGTGGCTATGACTTTGGTTTTGAAGGAGACTTCTAC AGGGCTGACCAGCCTCGCTCCCAGCCTTCACTCAGACCCAAGGACTATGAAGTTGATGCAACTCTGAAAT CTCTCAATAACCAGATTGAGACCCTTCTCACTCCTGAAGGCTCTAGAAAGAACCCTGCTCGCACGTGCCG GGACTTAAGACTCAGCCACCCAGAGTGGAACAGCGATTACTACTGGATTGACCCTAACCAAGGATGTACT ATGGATGCCATCAAAGTGTACTGTGATTTCTCTACTGGTGAAACCTGCATCCAGGCCCAACCTGTAAACA CCCCAGCGAAGAACTCATACAGCCGCGCCCAGGCCAACAAGCATGTCTGGTTAGGAGAGACTATCAATGG TGGCAGCCAGTTTGAATACAACGTAGAAGGGGTGTCCTCCAAGGAAATGGCAACTCAGCTCGCCTTCATG CGCCTGCTAGCCAACCGTGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGTACCTGGATG AGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTGAACTTGTTGCTGAGGG CAACAGCAGGTTCACCTACTCTGTCCTAGTCGATGGCTGCTCCAAAAAGACAAATGAATGGGGCAAGACA ATCATTGAATACAAAACAAATAAGCCATCTCGCCTGCCGTTCCTTGACATTGCACCTCTGGACATTGGTG GTGCTGACCAAGAATTCCGTGTGGAGGTTGGCCCCGTCTGTTTCAAATAAGTGAACTCAACATAAATTAA AAACCAAAAACCCCTGAAAAAACTTTCTCTTTGCCGTTTCCTCCTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3586272 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3586272 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7387 same embryo
	EMAGE:7385 same embryo
	EMAGE:7386 same embryo
	EMAGE:7383 same embryo
	EMAGE:7384 same embryo
	EurExpress:euxassay_004456 same experiment
	MGI:4823984 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source