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EMAGE:7386

Ndufs4 NADH dehydrogenase (ubiquinone) Fe-S protein 4 ( MGI:1343135)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7386 EMAGE:7386 EMAGE:7386 EMAGE:7386 EMAGE:7386
"Pseudo-wholemount" of euxassay_004465. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004465_01 euxassay_004465_02 euxassay_004465_03 euxassay_004465_04
EMAGE:7386 EMAGE:7386 EMAGE:7386 EMAGE:7386 EMAGE:7386
euxassay_004465_05 euxassay_004465_06 euxassay_004465_07 euxassay_004465_08 euxassay_004465_09
EMAGE:7386 EMAGE:7386 EMAGE:7386 EMAGE:7386 EMAGE:7386
euxassay_004465_10 euxassay_004465_11 euxassay_004465_12 euxassay_004465_13 euxassay_004465_14
EMAGE:7386 EMAGE:7386 EMAGE:7386 EMAGE:7386 EMAGE:7386
euxassay_004465_15 euxassay_004465_16 euxassay_004465_17 euxassay_004465_18 euxassay_004465_19
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euxassay_004465_20 euxassay_004465_21 euxassay_004465_22 euxassay_004465_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7386Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7386_wholemount_strong.wlz
7386_wholemount_moderate.wlz
7386_wholemount_weak.wlz
7386_wholemount_possible.wlz
7386_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7386_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6452
Entity Detected:Ndufs4, NADH dehydrogenase (ubiquinone) Fe-S protein 4 ( MGI:1343135)
Sequence:sense strand is shown

>T6452
CGGCGGTCTCAATGTCAGTGTCGTTGAGACAGGCGATGTTGGGGCGAAGGGCAATGGCTACAGCTGCCAT
TTCCGTCTGTAGAGTTCCATCCAGGTTCTTGAGCACATCCACTTGGAAGCTGGCAGACAACCAGACTCGG
GACACACAGCTTATAACAGTTGATGAGAAACTGGATATCACAACTTTAACTGGTGTTCCAGAAGAGCACA
TCAAAACCAGAAAGGTCAGAATCTTTGTTCCTGCTCGCAATAACATGCAGTCTGGAGTAAACAACACAAA
GAAATGGAAGATGGAGTTTGATACCAGAGAGAGATGGGAAAATCCTTTGATGGGTTGGGCATCAACCGCT
GACCCCCTCTCCAACATGGTTCTGACCTTCAGTGCCAAAGAAGATGCAATTGCCTTTGCAGAAAAAAACG
GATGGAGCTATGATGTGGAAGAGAAGAAGGTTCCGAAACCCAAGTCCAAGTCTTATGGTGCAAACTTTTC
TTGGAACAAAAGAACAAGAGTGTCTACAAAATAGGTTGGAGCTGGCTACATCTCTGCTTGACTGTGACTG
AAGTGTCAGCTGTGCACTATTTATAGTCCATGGATAATGCACCTCTTAATCTCCTAATAAATGTGACCTT
TAAACTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3585543 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585543 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7387 same embryo
 EMAGE:7385 same embryo
 EMAGE:7382 same embryo
 EMAGE:7383 same embryo
 EMAGE:7384 same embryo
 EurExpress:euxassay_004465 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS