EMAGE mouse gene expression spatial database

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EMAGE:7416

AU018091 expressed sequence AU018091 ( MGI:2142124)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005221_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7416_wholemount_strong.wlz

7416_wholemount_moderate.wlz

7416_wholemount_weak.wlz

7416_wholemount_possible.wlz

7416_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7416_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7635
Entity Detected:	AU018091, expressed sequence AU018091 ( MGI:2142124)
Sequence:	sense strand is shown >T7635 AAGTAATCCAGATGAGCTGTCAGCTGTCCCTCCCTGTTGGTCCCCACCCTACCCGCCAGGCACATTTCCG GTGGCTACTTGAATGCTGTGGGTCCTACCTCCTCCTACCTTAAAGAATTTTCAGCTTCTGTGCAGCACAG ATCTGGAGCTCTGCCCCAGGAACACATCCAGCGCTCCAGGATTTCATCCAGTGTCTGCAGAGTTTGGATC CCTCCACCAGGCACTAAAGATGTTATGGCAGAACCTCCATCAGTTTGGCCAGAAGTTGGTTCGCCGGCGG CCCCTGGCGGCAAACGAACAGTCTGAGTCTTCCTTGTCTCGATGTCTGACCACTTTGGACCTGGTGTCCT TGGGTGTGGGCAGTACCCTGGGTGCAGGCGTGTATGTCCTGGCCGGGGAGGTGGCCAGAGAGAAAGCTGG ACCGTCCATCATCATCTGCTTCTTAATTGCTGCCCTGTCTTCAGTGATGTCAGGACTCTGTTATGCGGAG TTTGGGGCCAGGGTACCATGCTCGGGTTCCGCATATCTCTACAGCTATGTCACAGTGGGCCAGCTGTTGG CTTTCATCACTGGCTGGAACTTAATACTCAACTATGTCATTGGTGCAGCCAGTGTGGCTCGGGCCTGGAG TGCAGCTTTTGATGGCCTCATAGGGAACCATATCTCCCAAGCCATGCAGACGACTTTCCCTATCCAAGTG CCCAGCTTTCTGGCCAAGTATCCAGACTTTTTTGCGCTTGGTCTGGTGATAGTTCTCACTGGAATCCTCG CCCTGGGAGCACGAGAGTCTGCCCTGGTCACCAGGGTTTTCACAGGAGTGAATCTTTTGGTGCTGTGTTT CGTCTCCCTCTCTGGCCTCATTAATGGCAAGCTACACAACTGGCAGCTTACAGAGGATGACTACAAACTG GCTCTGTTAGAATCCAACAACACTGACAGCTTGGGACCAATGGGCTCTGGAGGGTTCATGCCTTTTGGCT TGAAGGGGATTCTCCGTGGTACAGCAACATGTTTCTTTGCCTTCATTGGGTTCGACTGTATTGCGAGTAC AGGGGAAGAGGCCCGCTGTCCCCAGCGTTCCATTCCATTGGGCATCGTGACCTCACTCTTCATTTGCTTT CTGATGTATTTTGGTGTTTCGGGGGCACTCACTCTCATGATACCCTACTACCAGATTAACATCAACAGCC CCTTGCCCCAGGCATTTATCCACGTTGGATGGGGGCCTGCTCGCTATGCAGTAGCTGTGGGAACCTTATG TGCCCTTTCATCCAGCCTCATAGGTTCCATTTTCCCTGTGCCTCGGGTGGTGTATTCCATGGCAGAGGAT GGGCTCCTGTTCCGGAAACTTGCCTATGTTCATCCTCGAACACACACTCCGGTCCTAGCCACTGTCCTTT GTGGAATCATCGCAGCATTTATGGCTTTCCTCGTTGAGCTCAGTGATCTGGTGGACCTCTCGTCCATAGG GATCCTGCTGGCCTACACCTTAGTGACCTTCTCTGTTCTGATCCTCAGGTACCAGCCGGACCAGGTCTTA AGCTCTTGTAAGAGGGAGAAGTCTGAGAGTGCAGCTGTGGAGCTGGAGCCTGGACTTGAACCTTCTTCAT GCATGGAGCCTATTTCTCCAGAAGGGATGCCAGGGATTGCAAGGACACTCTGTATCCCCACGAACACCAC CCCAACTCTGAGATCTGGCCGGATTGTCTATGGATGTGCTTCCCTGCTCGTCTTTCTTCTGATGCTCCTG TGCCTGATCCTGACCCAGTGGTCCACACAGCTATTCTCTGGGGACCCAGTTTTGATTGCCATGGCTGCTT CACTATTGCTTCTTACTGTCGGAGTGATCACTGTCATCTGGAGACAGCCTCAGAGCACCACTCCTCTCCA CTTCAAGGTTCCAGCCCTGCCTGTGCTTCCTGCACTGAGTATCTTTGTGAATATCTACCTGATGATGCAA ATGACTTCTGGGACCTGGTTTCGCTTTGGAATCTGGATGGTGATCGGATTTGCTATCTACTTTGGATATG GGATACAGCACAGCTTGGAAGAGAAGAATGACCAACAGCCAACAGCCTCAAGCTCCCAGACTCTCCAGGA ACTCCAGGAACATACTCCCAGTGTTGACTTACCTTAACTGGAGAAACGGGATGCTTCCAGGACCCCATGG GCATCAGTTTCCTTCCCTTCCCTTCCCTTTCCCTTTCCTCCCTCTTTCCCTTCCCCCTTTTCCTTTCCTC CCTCTTTCCCTTCCTTTTATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGCCTG TGTGTGTGTGTGTGGGGCGGGTTGGGAAGGTAGTACTGGGAATTGAACTTAGAGTCTTGTGCGTCTGAGG CAAATGCTCCATCACTGACCTAATCTCTTGTAGGTATCTTTAGGTCAAGGAAAAGTTTGAATATTTGTGG GCTGTAGATGGGACTTCTTTTCTTACTTTCTTTTTTTTCTTCTTCTCTTTTTGGGACTTATATCATTGTA ATTTTGTGAGCAAATTCCTTAACCTTTTTTTTTTTTGGTGTGTGTATTGAAATTGGATCCATGATTTTGC ATATGCTAGGCAAGTAGTACTCTACCACTGAGCCACATCCCTAGTCCCTTTAGTTATTTAACTGACAGCA AAATTTAGGTATTTACATATTATAGAATGGTTAAAAAAACTGATTAATATATGTTTCTTTTTATGTTTGG GCAGTTAGTCTTCTATGGCTAAGGTCAGACTTGAACTTGATCTTCTGAACATTATTATTTATGGAGAGAG AGAAAACACATGTGTCACAGTGTTTGTGGAGTTCAAAGGACAACTTTATAGGTTCCTTCTCCCTTTCCAC TTTTCATGGATTGAACTCAGGTATTTAGGCTTTTGTGGCAACAGCTTTACCCACTGAGGCATCTTGTTGG CCCCTGTATCCCCTCCCCCACCCATAAACAGTATCATAACTGAGAACTATCATGAACTCATGACCCTTCT GTCACCAGTTCTCCAAGTGTTGGTATTATAAGTGTGGACTGTGCTTGTTGTTGTTTTTGTTCTGTTTTTC AAGACACAGGGTTTCTTGATGTAGTTCTGGCTATCCCTGTAACTTACTACAGTAAGTTAGATCAAGCTGG CCTTGAACACAGAGATCTCCCCCATGAGTGCTTAGATTAAAGGCATGTGCCACCTCTGGCCTATCTTCAT GCTCAGTTACTGTGGTGCTAAACATTATGCCAGGGTTTTAAGCACATTTGGACAACCAATTTACCAACTG AGCTACACCCCCCAACCCTATGTTTATGTTTTGTGATGAAAACATTAAATTTTTTTAAACATTTTACAGA TCTATAGTGCATTGTTCATCTGTGGCAACATGTATATGAACTTGGAGGATGTATATTATGTTAAGTGAAA TAAATGAGGTTTGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30112559 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30112559 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7413 same embryo
	EMAGE:7414 same embryo
	EMAGE:7412 same embryo
	EMAGE:7417 same embryo
	EMAGE:7415 same embryo
	EurExpress:euxassay_005221 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source