EMAGE mouse gene expression spatial database

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EMAGE:7453

Pdzk1ip1 PDZK1 interacting protein 1 ( MGI:1914432)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004424. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004424_01	euxassay_004424_02	euxassay_004424_03	euxassay_004424_04

euxassay_004424_05	euxassay_004424_06	euxassay_004424_07	euxassay_004424_08	euxassay_004424_09

euxassay_004424_10	euxassay_004424_11	euxassay_004424_12	euxassay_004424_13	euxassay_004424_14

euxassay_004424_15	euxassay_004424_16	euxassay_004424_17	euxassay_004424_18	euxassay_004424_19

euxassay_004424_20	euxassay_004424_21	euxassay_004424_22	euxassay_004424_23	euxassay_004424_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7453_wholemount_strong.wlz

7453_wholemount_moderate.wlz

7453_wholemount_weak.wlz

7453_wholemount_possible.wlz

7453_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7453_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

anterior naris

moderate

regional

moderate expression: see section 08 09 11 12

external naris

moderate

regional

moderate expression: see section 08 13

kidney calyx

moderate

regional

moderate expression: see section 06 07 09 16 17 18 19 weak expression: see section 10 15

kidney pelvis

moderate

regional

moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7573
Entity Detected:	Pdzk1ip1, PDZK1 interacting protein 1 ( MGI:1914432)
Sequence:	sense strand is shown >T7573 AAAACACCAGCAGTGGACCGAGGTCCTACAGCTGCCTGCAGCCATGTTGGCCTTCAGTCTGCTCGTGCTG GGTCTGCTCGCGGAAGTGGCACCTGCCAGCTGTCAACAAGGTCTAGGAAACCTTCAGCCCTGGATGCAAG GCCTCATTGCTGTCGCTGTGTTCTTGGTCCTTGTTGCAATCGTCTTCGCCGTCAACCACTTCTGGTGCCA GGAGGAGCCGGAGCCTGGGAGCACAGTGATGATCATTGGAAACAAGGCAGATGGGGTCCTGGTGGGAATG GATGGCAGATACTCCTCAATGGCATCTGGTTTTAGGTCCAGCGAGCACAAGAATGCCTACGAGAATGTTC TGGAGGAAGAGGGCAGGGTCCGCAGCACACCCATGTGACAAGCTTCTCTGTGGCCCTAGTCCCCAGGCTA CAGGGAACATAGAGTTGATCCCCACCATCTCACCCAGCCACTGCTCTACAGGAATCTACTGAAACAAGCT GACTCCCTCACCTCTCTAGAATCTAGAATCACAGTCATCTCAGGCCAGGACTCAGACCAAGCCCAGCACA CCATGTGCTGTGACCCTGGGCTTTCCTGGGTCTTCTACCCAGTTCTGAGGAGCCTTGTGAAGGGTGGCTC AGCTCCTCCCCTCCCCTTGCTCCTGCAACTATGGAAGTGGCTCTTATTTCTGTGAAATAAAGACTTTTTA TACTTCTGGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241868 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241868 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7451 same embryo
	EMAGE:7449 same embryo
	EMAGE:7452 same embryo
	EMAGE:7448 same embryo
	EMAGE:7450 same embryo
	EurExpress:euxassay_004424 same experiment
	MGI:4827144 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source