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EMAGE:7467

Pemt phosphatidylethanolamine N-methyltransferase ( MGI:104535)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7467
euxassay_004562_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7467Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7467_wholemount_weak.wlz
7467_wholemount_possible.wlz
7467_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7467_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7435
Entity Detected:Pemt, phosphatidylethanolamine N-methyltransferase ( MGI:104535)
Sequence:sense strand is shown

>T7435
CATTTCCTTCTGGTTCTGGCTGATCTCTTCGTTATGAGCTGGCTGCTGGGTTACATGGACCCCACAGAGC
CCAGCTTTGTGGCGGCTGTGATCACCATTGTGTTCAACCCACTCTTCTGGAATGTGGTAGCGAGATGGGA
GCAGAGAACTCGGAAGCTGAGCAGAGCCTTCGGCTCCCCCCACCTAGCCTGCTATTCCCTGGGCATCTGC
ATCCTGCTTTTGAACATCCTCCGCTCCCACTGCTTCACACAGGCTATGATGAGCCAGCCCAAGATGGAGG
GCCTGGACAACCACACCACCTACTTCCTGGGCCTTGCATTCCTAGGTTGGGGATTCGTGTTTGTGCTGTC
CAGCTTCTATGCACTGGGGTTCACTGGGACCTTTCTAGGTGACTACTTTGGGATCCTCAAGGAGTCCAGA
GTGACCACATTTCCCTTCAGCGTGCTGGATAACCCCATGTACTGGGGCAGTACAGCCAACTACCTAGGCT
GGGCACTCATGCATGCTAGTCCCACAGGCCTGCTGTTGACGGTGCTGGTGGCAATTGTCTACGTGGTTGC
TCTCCTATATGAAGAGCCCTTCACTGCTGAGATCTACCGACAGAAAGCTACCAGGTTGCACAAAAGAAGC
TGACAGGGCCATGAAGGATCTTTGGAAAGCTGGACTGGCCTCTCGGCTGCCCCAAGCAACAACCCTTCCC
AGGGAGAGCAGCACTGGCCACTGTACCTGTGCCTTAGAAACCAGTCATGGGGGCCTCAGGCATTATGCTG
TGTGACTGCTGAGACCCCCATCCCCACCATGTCCCGACACACTAATAAAGGCATTGTGACCTCAAAAAAA
AAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4196324 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4196324 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7468 same embryo
 EMAGE:7465 same embryo
 EMAGE:7466 same embryo
 EMAGE:7470 same embryo
 EMAGE:7469 same embryo
 EurExpress:euxassay_004562 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS