EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7470

Qprt quinolinate phosphoribosyltransferase ( MGI:1914625)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004564. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004564_01	euxassay_004564_02	euxassay_004564_03	euxassay_004564_04

euxassay_004564_05	euxassay_004564_06	euxassay_004564_07	euxassay_004564_08	euxassay_004564_09

euxassay_004564_10	euxassay_004564_11	euxassay_004564_12	euxassay_004564_13	euxassay_004564_14

euxassay_004564_15	euxassay_004564_16	euxassay_004564_17	euxassay_004564_18	euxassay_004564_19

euxassay_004564_20	euxassay_004564_21	euxassay_004564_22	euxassay_004564_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7470_wholemount_strong.wlz

7470_wholemount_moderate.wlz

7470_wholemount_weak.wlz

7470_wholemount_possible.wlz

7470_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7470_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mandible

moderate

single cell

moderate expression: see section 05 06 07 15 17 18 19 20

maxilla

moderate

single cell

moderate expression: see section 05 06 15 16 17 18 19 20

kidney calyx

moderate

regional

moderate expression: see section 06 07 08 17 18 weak expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7433
Entity Detected:	Qprt, quinolinate phosphoribosyltransferase ( MGI:1914625)
Sequence:	sense strand is shown >T7433 GAACTGTGGTGAACACACTGACCCGCTGGCTGCTCCAAGTCACCATGGACCCTGAAGGCCTGCAGCTCCT GTTACCCCCTACAACCTTGGCAGCCCTGGCGAACAGCTGGCTGCAAGAGGACTGCCCGGGCCTCAATTTT GCATCCTTGGTTACCGGGTCGGCACCCTCGCAGGCTGTGCTGTGGGCCAAATCTCCTGGGGTTCTGGCAG GGCGACCCTTCTTTGACGCCATCTTCACTCAACTCAACTGCCAAGTGTCCTGGTTCCTCCCGGAGGGATC GAAGCTGGTACCTGTGGTCAAGGTGGCAGAGGTCAAGGGACCTGCCCACCACCTGCTCTTGGGGGAACGG GTGGCTCTTAACACCCTGGCCCGCTGCAGTGGGATTGCCAGCGCGGCAGCGACAGCTGTGGAGGTGGCCA GGAGCACCGGCTGGACTGGACACGTGGCGGGCACGAGGAAGACGACACCTGGGTTCCGACTGGTGGAGAA GTATGGGCTCCAAGTAGGCGGGGCAGCATGCCACCGCTACGACCTGGGAGGGATGGTGATGGTGAAAGAC AACCATGTAGTGGCGGCCGGCAGCATGGAAAGGGCAGTGCTGAAGGCTCGGCAGGCAGCCGGGTTTTCCC TGAAGGTAGAGGTGGAATGTAGCAGCCTGGAGGAGGCCTTTCGGGCAGCAGAGGCTGGGGCTGACCTGGT AATGCTGGACAACTTTAAGCCTGAGGAGCTGCACCCCACAGCAGCTACACTTAAAGCCAGATTCCCCAGC GTGTCGGTGGAAGCCAGTGGGGGCGTCACACTGGACAACCTCACCCAGTTCTGTGGGACACACATTGACG TCATCTCGTTGGGAATGCTGACCCAGGCTGCTCCAGCCCTGGATTTTTCCCTCAAACTGTTTGCTGAAGG AGATACCCCAGTGCCTCACGCTCGCCGGTTCTAAAGCCGAAGAAGATGCCACCAGAATGGACTACTGCGG CTTGTTGGGCACCTTGGGTCACATGTCTTTAGATTCAGAGGCCAATAGGACATATCTAGGCTTTTTACCT AGGCTTCCCACTAGCTCTGCCATCTCCAGCTTGTGTGACTTGCCAGGGCTGACTTTGATTTTGCTTGCCT CGGTTTTCTCATCTGTGAAATAGGTCTAATAAATAAAATCAACAGCCTTACTGGGCTGTTGATGATAATA ACTACTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4196244 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4196244 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7468 same embryo
	EMAGE:7465 same embryo
	EMAGE:7466 same embryo
	EMAGE:7467 same embryo
	EMAGE:7469 same embryo
	EurExpress:euxassay_004564 same experiment
	MGI:4827559 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source