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EMAGE:751

Notch2 Notch gene homolog 2 (Drosophila) ( MGI:97364)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:751
Fig 4G Przemeck et al, 2003 [PMID:12441287] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: the red circle indicates the approximate position of the node; the asterisk indicates the position of the allantois.
Expression Pattern Description
Spatial Annotation:
EMAGE:751Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
751_wholemount_strong_3D_1.wlz
751_wholemount_moderate_3D_1.wlz
751_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:751_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
node
detected detected
notochordal process
detected detected
embryo
detected detected
regionalExpression in single stripes lateral to each side of the node
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2446000
Entity Detected:Notch2, Notch gene homolog 2 (Drosophila) ( MGI:97364)
Notes:The Notch2 probe used this study by Przemeck et al, 2003 [PMID:12441287] is indicated as that described by Larsson et al, 1994 [PMID:7698746] . The only mouse Notch2 clone mentioned by Larsson et al, is one described by Lardelli et al, 1994 [PMID:7918097] that was used to screen a human cDNA library. Lardelli et al, 1994 describe Notch3, not Notch2 however Ladelli and Lendahl, 1993 [PMID:8440332] which is also cited by Larsson et al, 1994 do describe two Notch2 clones. It is not clear which one was used here.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:129S1/Sv x 129X1/SvJ x C57BL/6J
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Przemeck et al, 2003 [PMID:12441287] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00176] [ PMID:12441287] Przemeck GK, Heinzmann U, Beckers J, Hrabe de Angelis M 2003 Node and midline defects are associated with left-right development in Delta1 mutant embryos. Development (130):3-13
 [ doi:10.1006/geno.1994.1613] [ PMID:7698746] Larsson C, Lardelli M, White I, Lendahl U 1994 The human NOTCH1, 2, and 3 genes are located at chromosome positions 9q34, 1p13-p11, and 19p13.2-p13.1 in regions of neoplasia-associated translocation. Genomics (24):253-8
 [ doi:10.1016/0925-4773(94)90081-7] [ PMID:7918097] Lardelli M, Dahlstrand J, Lendahl U 1994 The novel Notch homologue mouse Notch 3 lacks specific epidermal growth factor-repeats and is expressed in proliferating neuroepithelium. Mech Dev (46):123-36
 [ doi:10.1006/excr.1993.1044] [ PMID:8440332] Lardelli M, Lendahl U 1993 Motch A and Motch B - two mouse Notch homologues coexpressed in a wide variety of tissues. Exp Cell Res (204):364-72
Links:MGI:2449022 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI