EMAGE mouse gene expression spatial database

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EMAGE:7531

Sdhaf2 succinate dehydrogenase complex assembly factor 2 ( MGI:1913322)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004602. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004602_01	euxassay_004602_02	euxassay_004602_03	euxassay_004602_04

euxassay_004602_05	euxassay_004602_06	euxassay_004602_07	euxassay_004602_08	euxassay_004602_09

euxassay_004602_10	euxassay_004602_11	euxassay_004602_12	euxassay_004602_13	euxassay_004602_14

euxassay_004602_15	euxassay_004602_16	euxassay_004602_17	euxassay_004602_18	euxassay_004602_19

euxassay_004602_20	euxassay_004602_21	euxassay_004602_22	euxassay_004602_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7531_wholemount_strong.wlz

7531_wholemount_moderate.wlz

7531_wholemount_weak.wlz

7531_wholemount_possible.wlz

7531_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7531_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7669
Entity Detected:	Sdhaf2, succinate dehydrogenase complex assembly factor 2 ( MGI:1913322)
Sequence:	sense strand is shown >T7669 AAGAGGTAATCCGAGAGATGGCGGTGGTCACCTTGATCCCGACGCTGGCGCGGGCACTGTCAAAGCACAG TCTGCTGTCTCCCTTGCCTAGTGTGACATCATTCAGACGCTTCTACAGAGGTGACAGCCCAACAGACTCT CAAAAAGACATGATTGAAATCCCTTTGCCTCCGTGGCAGGAGAGAACTGATGAATCCATAGAAACCAAAA GAGCCCGCTTACTCTATGAGAGCAGAAAGAGGGGGATGCTGGAGAACTGCATTCTCCTTAGCCTGTTTGC TAAAGAGTATCTGCACAACATGACAGAGAAGCAGCTGAACCTCTATGATCGCCTGATTAACGAGCCTAGC AATGACTGGGATATTTACTACTGGGCCACAGAAGCAAAACCAGCCCCAGAAATATTTGAAAATGAAGTCA TGGAATTGCTGAGAGAATTCGCCAAAAACAAAAACAAGGAGCAGAGACTGCGTGCCCCAGATCTGGAGTA TCTCTTTGAAAAGCCTCATTAAGCTGGGTACCACTGCCTGTCATGGTGGTCAGTATATGGATAGAAACTG CTTCTGGCACTGACCTTGGCCTCCTGCTTCTCCTTAGACTCTTGAATCTCTCCCAGGAAAGACATGTAAT ATGTTAGCTGACTCATTCTAACCTGTGCTCGTCTGTACAGCACAGGTCAGACCCCTTACCTGGCTGCTAA GTGCTGTGCTGCAGGTCTGCCACAAGAGGGCACTATAGGGGAAGTGATTCTGCTGCCTCAGCCCCAACAG TACAAAAGACACTGCTGCTGAAGGAAGAGCTGACCCTATCGTCTATCCAGTGACCCTGGGCTTTGTTAAG ATAAAACTGCTCCCTTCCAGCGTGATAAGTCCTTTGCTAAGGAATGTCGGTCCAAGCCCCTGAACTGAGC CATCAAAGGCACAGCAAGCTGAATGAGGCACCAAAGCAGGAATATTGTGGGCTTACTAAGTCAACATCTT CATCAAATTTTCACAACATACATGTTTTAAACTGAAAGATAGTTGAATATTGAAGAATATTAAGAGAAGA AAATTGACAGTTCTCTAACCCTCACAATTATCTTCATTTTTGTCTGTTTCTATGCAACCTATAGTCATGC TTATAATGGTGTTTTACAAAATTATAGTCATAAGTTTTCTGACTTTGTGGCATTGAGGCAAACTTTCTCC TCATTCTGACAGCCTTTCAAATTATTTTAGTTGACCAAATAACAGTGACACATAAGAAGTAACATTTTAA CTGCGTGTGATAGGTCACACCTATGATCCTAGCCCTTGGAGAACTGAGGTAGGAACATCACTAAGAATTT GAGGCCAGCCTGGACCACACGGTTAGAATGCAGTAGCTAGGAGAGCTGTGCAGTCCTGAAGGCATCCTTT CTGCAGGAGCTCTGCCCTCAGCTGCAAAGAGGCAGTCACTCTGGCCGGTATGTGAGTGTTGGCTAGAGAA AGCAGGCCAGAACTGAGAAGCCTGGGTGGGTAACTACAAGAGCTATAGGAAAAAGTTCCCTGGCCAGGGA GGGAAGCGGGAATCAGCATCAGTTACCTCACAACGCTCCCACAGACAAATAGCATTGGGCCTACTTAAGG ACAAGAGATTGCCTTGGAAGGAGTCAGCAATGCATCCATGATCAAAAAGCCAGGATTTGGGGCTGGAGAG ATGGCTCAGCGGTTAAGAGCACTGGCTGGCTGTTCTTCTAGAGGACCTGATTCAGTCCCTAGCACCCACA AAGCAGCTCACAACTGTCTGTAACTCTAGTTTCAAGAGGATCTGACACAGATGCAGGTGAAACACCAACG AATATTTTTTAAAAATCATAAAAAGGTTAATAAAAAGACGAAAGCTGGAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30441476 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30441476 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7534 same embryo
	EMAGE:7529 same embryo
	EMAGE:7533 same embryo
	EMAGE:7532 same embryo
	EMAGE:7530 same embryo
	EurExpress:euxassay_004602 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source