EMAGE mouse gene expression spatial database

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EMAGE:7533

Srsf3 serine/arginine-rich splicing factor 3 ( MGI:98285)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004629_01	euxassay_004629_02	euxassay_004629_03	euxassay_004629_04	euxassay_004629_05

euxassay_004629_06	euxassay_004629_07	euxassay_004629_08	euxassay_004629_09	euxassay_004629_10

euxassay_004629_11	euxassay_004629_12	euxassay_004629_13	euxassay_004629_14	euxassay_004629_15

euxassay_004629_16	euxassay_004629_17	euxassay_004629_18	euxassay_004629_19	euxassay_004629_20

euxassay_004629_21	euxassay_004629_22	euxassay_004629_23	euxassay_004629_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7533_wholemount_strong.wlz

7533_wholemount_moderate.wlz

7533_wholemount_weak.wlz

7533_wholemount_possible.wlz

7533_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7533_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7625
Entity Detected:	Srsf3, serine/arginine-rich splicing factor 3 ( MGI:98285)
Sequence:	sense strand is shown >T7625 AGAATCTGTAGGAGCAGAACCAGAGGGAAGCGGGTGGGCCTGTCGGAGCGTTAGGATTTGAGCTTGGGCC TTTTGAACCCAGGATCTCGAAATGCATCGTGATTCCTGTCCCTTGGATTGTAAGGTTTATGTAGGTAATC TTGGAAATAATGGAAACAAGACTGAATTAGAACGGGCTTTTGGCTATTATGGACCACTCAGAAGTGTGTG GGTTGCTCGAAACCCTCCTGGCTTTGCTTTCGTCGAATTTGAGGATCCCCGAGATGCTGCTGATGCTGTC CGGGAACTAGATGGAAGAACACTGTGTGGCTGCCGTGTAAGAGTGGAACTGTCGAATGGTGAAAAGAGAA GTCGGAATCGTGGGCCGCCTCCCTCTTGGGGTCGTCGTCCTCGAGATGATTACCGCAGGAGGAGTCCTCC ACCTCGGCGCAGATCCCCAAGAAGGAGAAGCTTTTCCCGAAGCCGGAGCAGGTCACTTTCTAGAGATAGG AGAAGAGAAAGGTCTCTGTCTCGTGAGAGAAATCACAAGCCGTCTCGATCCTTCTCTAGGTCTCGTAGCC GATCTAGGTCAAATGAAAGGAAATAGAAGACCAGTTTGCAAAAGTGGTGTACAGGAAATAACTTCATCTG ACAGGAGTATGTACAGGAAATTAAAGTTTTGTTTGAGACTTCATAAGCTTGGTGCATTTTTAAGATGGTT TAGCTGTTTAAATTTGTTTTGTCTCTTGGAACAGTGACACACAAAACAATGTAATTCTCTATGGTTTTCA GATGGATCATAAGAGGCACGTGATATCAAGAATTGTTACTTTACAATGTTCCCTTAAGCAAGATTTAATT TTCTTTGAATTTTAGTTTTTCATAGACTGAAATAAACCTTAGGTCCTGCCCAGTTTTAAGTGTGATGTAC TAATGATATAAAGCAACTGGCGGAAATTGAAAGAAGCTATAGTCCTCTAGTAGCTGAGACACTGTGGCAC TGTGGGTGGAATGATAAAGCGGTGTTTAAGAGCTGCTGTGAACACAAGCCAACAGATAAGGGTAGGAACC ACACTGAAGATTTGCAAAGGGGTTCCTTCCTGGTTTCTCTATGGGGATGCAGAGCTATTGTAACGTCTTT ATTTGGAAATGTAAAACTCAGATGCCAGTGTCCTTTCAGTTTAAGGGTACATTGTAGAGCTCAACTTTTC AGTTACTGTGCAAGATTGTTTTTCATGCTGTCATCTGTAATATGTTTGTGAGAATCTTTGGGATTAAAGT TTTGGTTACAAATTGTTGTTTAACTTGAAAGCCTGTTTTTCCTTGCACACTCAAAATCTGTGAGCTTGGT ATCAAGTCCAGGTAAAACATTCCTATTGGAAGCCATACTTATATTTTCTTGTAAAGTGCTTTTGAATTAA TAAAGTACTAGCATAATTGTGTAGTAGTCAGTCGAGCGAACCACTGTTGCCATTGTTCTTATCCCTGGGA AAGTAGTTGGTTACCCAGTTCATTCTTTGTAAGAAACACAACTGAGAAGCACTACATTAGTGAAGGAATT TTTTTTTCCCCTTTGCTTGTCACTTATGAGAGTTAGCCATTTTTTAGGTGTTGAAGCCAGTTCAGGATTA TGGCTGTTAGTGACTAACATTTTAAAGTAAGCTCCAGTATGGTAGGTATATTGTAATTCCCTTAAGTTGC CCATAAAAAGTGGCTTTGATCTTCAGCATGAAAACATTCAAAATTTTAAAATTTATTTTATAACTTGAGG TGTTGCATGGGATTCCAAATTGATCCATCATGATGTAAAATCCCCAATATGGTTAATTGTAATGATGCAC AGTTGAAATGGAGGCATGCATATCCTTTCTCTTAGAATCTGGAGGAGTTGTGGTTTCAGGTATTTGTGTG CAACTAGATTAAATCATAATGCAACAGTCTTGTGGCTTAAGTTTCCTTAAATGTGTTCTTTTGAGATAAG ATTCTACTGTACTGACTTGGTCTATGTAAGAGCCATTGCACTAAGTTAAATCTGTAAGCTTATAAATCTC CCCCATTGTTATCTTTAAACAATAAATTTCAGTAAAAAAGGAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30441615 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30441615 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7531 same embryo
	EMAGE:7534 same embryo
	EMAGE:7529 same embryo
	EMAGE:7532 same embryo
	EMAGE:7530 same embryo
	EurExpress:euxassay_004629 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source