EMAGE mouse gene expression spatial database

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EMAGE:7539

Snx24 sorting nexing 24 ( MGI:1916476)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004659. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004659_01	euxassay_004659_02	euxassay_004659_03	euxassay_004659_04

euxassay_004659_05	euxassay_004659_06	euxassay_004659_07	euxassay_004659_08	euxassay_004659_10

euxassay_004659_11	euxassay_004659_12	euxassay_004659_13	euxassay_004659_14	euxassay_004659_15

euxassay_004659_16	euxassay_004659_17	euxassay_004659_18	euxassay_004659_19	euxassay_004659_20

euxassay_004659_21	euxassay_004659_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7539_wholemount_strong.wlz

7539_wholemount_moderate.wlz

7539_wholemount_weak.wlz

7539_wholemount_possible.wlz

7539_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7539_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7696
Entity Detected:	Snx24, sorting nexing 24 ( MGI:1916476)
Sequence:	sense strand is shown >T7696 GATCGCCAACCTCGCGCCGCCGACTAAGGTCCGGGCCGCGCACCTCGGGCCGCGCGCGCGCCGGGAGGGG AGCGGGAGGCGGGGCGGTCTACGCGGTGCGGCGGAGCGGTGAGTGGCGCGGCCCCGCCGCGGCCATGGAG GTCTACATCCCGTCCTTTCGCCACGAGGACAGCGATCTGGAGCGCGGCTACACGGTGTTTAAAATAGAAG TGCTAATGAACGGAAGAAAACATTTTGTTGAGAAACGGTACAGCGAATTCCATGCCTTGCACAAAAAGCT TAAGAAATGTATAAAGACTCCAGAAATCCCTTCTAAACACGTCAGGAACTGGGTCCCCAAAGTCTTGGAA CAACGGCGGCAAGGCCTGGAAACGTATTTACAGGCCGTCATTTTGGAGAATGAAGAGCTTCCCAAACTCT TCCTTGACTTCCTCAATGTACGGCACTTGCCCTCTCTACCGAAAGCAGAAAGCTGTGGATCTTTCGATGA GACAGAGTCTGAAGAGTCAAGCAAGCTGTCCCACCAGCCGGTGCTGCTGTTCCTTGGGGATCCATACGTC TTGCCTGCAGCCAGCGATTTTCCAAATGTGGTCATCGAAGGAGTTCTTCATGGGATATTTTTCTCTCACC TGCAGCCCAGGTAGAAACCCTACCCGGCTGGAAGAAGCTGAAGTGAGTGTCGATGCCGATGGCAAGAAAA TCGATATCTCTACCAAGTTAACCGAAACTCTGAGACACAATAGCTCTAGCTAGTGGGAAAATTCACAGTT CCAGCTTCCTTTGGAGCAGGGACATTTCTATTAGAATGGTGCTTTTAAAATTAGAAACTGAACCGGGGTG GGGTCAGTTTAAGACCAATTATGTGAGAAGTGAAAGGGAAATTACAAGAGAGGTTAGGAAAAGGAGGCCA CAGTGGATTACTGGGAGTGTCACAAAATAGAGCCAGCCTCTGCTTCAGTCACACTACCTGCTAATTGGAA ATCATGTCTTGAGTCACGCTGTGATACTGAACAACTTCGGTGGCCTTTTGCTCAGACCTTGGACACCCTC ATCTTTGCGCCATGAGAGTTCTCTCAGTGGGGGATACTGTATTCTGGAGTGTGGGCACCTGAAGAGTATC CCGGAGCCCACTGTGCCGGTCTGAGGATCCAGTTATACTGACAGTAGAACACACTTTCATTTCCATTACA GTGGGGATGCCTCCCACCTACAGATTAGCTGCCAGCATCAGTGCTGGAGATGAAATGGGTGTTGGGACCA CCCTTGAGAGCACTCTAGAAAGGCGCTGTATGGGAAGTCTGACCTCCCTCATCCTCTCCCTCCAGTACTG GAAAGTATGAGTGCGCCTCTGAAAACCAGCTGACACCCCTGGCGAAGTTCCAGGGGCCGTTCCATATAGA AAGTACGTACTCTAAACACTAAAATCAGACTGCAAAATTCTTGTCCTGCAGGAATGGGACATGGGGTCAC AGTGGCCACAATAGGAGCAAACAGACTGTTTGTGGCACGGTTCCCTCAATGGTGTACACCCACGCCGCTG TTCAGGTTCCTCTTCACTGACATCACGTATGGATTAAAGTGAGTTAGATGCTCGATGCTGTCTCTCCACT GGATGTCAAACAGACGGTCATCTTTCACTTAGTGATGGGGTAGAGTGCGTATGTCCTACCTGTGTGTAAA TGCTGATGAACAGCCTACTTACAGCTATGAATTTTCATAAATTTAAATGTGGGAGGAAATATTTAAAATT AAAATACCATGTCATGAATTTTTATTAGAGCCTAAACCTACAGATTGAAAGCCTGCTGCAAACTTTTAAA AAATAACATTTTTATGATGGATTGTGTAATCACATGTTGGAAATAAAGTAACCAAGTGTTATCAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6306904 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6306904 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7535 same embryo
	EMAGE:7537 same embryo
	EMAGE:7540 same embryo
	EMAGE:7536 same embryo
	EMAGE:7538 same embryo
	EurExpress:euxassay_004659 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source