EMAGE mouse gene expression spatial database

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EMAGE:7560

AI593442 expressed sequence AI593442 ( MGI:2143099)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007649. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007649_01	euxassay_007649_02	euxassay_007649_03	euxassay_007649_04

euxassay_007649_05	euxassay_007649_06	euxassay_007649_07	euxassay_007649_08	euxassay_007649_09

euxassay_007649_10	euxassay_007649_11	euxassay_007649_12	euxassay_007649_13	euxassay_007649_14

euxassay_007649_15	euxassay_007649_16	euxassay_007649_17	euxassay_007649_18	euxassay_007649_19

euxassay_007649_20	euxassay_007649_21	euxassay_007649_22	euxassay_007649_23	euxassay_007649_24

euxassay_007649_25	euxassay_007649_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7560_wholemount_strong.wlz

7560_wholemount_moderate.wlz

7560_wholemount_weak.wlz

7560_wholemount_possible.wlz

7560_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7560_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 18 weak expression: see section 10 11 17

rest of cerebellum mantle layer

weak

regional

weak expression: see section 09 10 21 22 23

pons mantle layer

weak

regional

weak expression: see section 10 15 18 20 21

ventral grey horn

moderate

regional

moderate expression: see section 11 12 16 18 weak expression: see section 13 14 15

bladder

weak

regional

Expression in the fundus region.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35905
Entity Detected:	AI593442, expressed sequence AI593442 ( MGI:2143099)
Sequence:	sense strand is shown >T35905 TCCATGCCTGCATCTGATATACAGCAAAGGGGATCTTTCTTCTTCCCAAGTCTGGCCTAATTAACCTCCC TGAACACATAGGAAATGTTAAGGGAAAAGGAAAGCATGAGAGAAGATAAATCTCTTGTCCTCTCTTTAAA TGTCAGATAAGTCCCTCTATGTTAGACTCTGCTGTTTAGTGAAGGGCAGTGGGACCCCTACATATATCCA TCTCCCAAGCCACTAGCTCCCCTCTATGCTCTGTCTTATTTCAAGTTGTATGTGGTTATTATCCCGAGAA ATGATTGCCTCTAATGTTTTGGTTACATATAAAGTTTTCCAAGCTCAGTCTGTATCTTTATAAAATAATT TAATAAGGTTGATTTAGTCACCCATAGATATCATCCAAGTCCTTTCTGAAGCACAGAAGACCATCGTTTA AGCATGCCATGTTGTATCATTAGGAAGATCAGGTATAATCTTTGGATACAATATATTAAACAATGAACCA GATTCTCTCCAGTGCCTTAGTCACTTCCTAGTAACAGGTCAGAGTGCATTCAGTCCCTCGGGCCACCAAG GATGCTGTTAGTGTATCAGAGCTCTACACTGTACAACAGAATGGCTAAGGCACTGTGAAGGAGAATATCC AT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70582. Forward Primer - name:070582_F_cDNA_AI593442, sequence:TCCATGCCTGCATCTGATATAC; Reverse Primer - name:070582_N_SP6_cDNA_AI593442, sequence:ATGGATATTCTCCTTCACAGTGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7565 same embryo
	EMAGE:7563 same embryo
	EMAGE:7564 same embryo
	EMAGE:7561 same embryo
	EMAGE:7562 same embryo
	EurExpress:euxassay_007649 same experiment
	MGI:4823046 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source