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EMAGE:7562

Gfod1 glucose-fructose oxidoreductase domain containing 1 ( MGI:2145304)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562
"Pseudo-wholemount" of euxassay_007665. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007665_01 euxassay_007665_02 euxassay_007665_03 euxassay_007665_04
EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562
euxassay_007665_05 euxassay_007665_06 euxassay_007665_07 euxassay_007665_08 euxassay_007665_09
EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562
euxassay_007665_10 euxassay_007665_11 euxassay_007665_12 euxassay_007665_13 euxassay_007665_14
EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562
euxassay_007665_15 euxassay_007665_16 euxassay_007665_17 euxassay_007665_18 euxassay_007665_19
EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562 EMAGE:7562
euxassay_007665_20 euxassay_007665_21 euxassay_007665_22 euxassay_007665_23 euxassay_007665_24
EMAGE:7562 EMAGE:7562
euxassay_007665_25 euxassay_007665_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7562Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7562_wholemount_strong.wlz
7562_wholemount_moderate.wlz
7562_wholemount_weak.wlz
7562_wholemount_possible.wlz
7562_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7562_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35910
Entity Detected:Gfod1, glucose-fructose oxidoreductase domain containing 1 ( MGI:2145304)
Sequence:sense strand is shown

>T35910
GGGATAGAACCCCTAGCTCTGTGCATGGCAGGCAAGCTCTCTACCGCTCAGCCATACCTCCAACCTCAAT
TAAATCTTGAAAGTATTCAGAAAACCTTGTGACAACAACAAAAGGGCAATCTCTAATTATGCATCTCTGG
AGGCTAGGGGACAGAGAGCCAGGTATTCACCCTGAGAAGAACGCAAGGCCCACTCTACTCATAGGCTATT
GTCCTATTGCTGGATGTGAACTTTCACTCTGACTAGCCTGTGACTCTGGCTCCATTCACTGGTGATTTCT
TTTGCCCACCAAAACCCCGGATCTTGCAGGACAGGATGACACTGGGGAACTAAGTACCTGTCAGACAATC
CTATTTGTTTCCTTTGGGCAAGGGAGGTGTCAGAAGTCTGAAGTATGGCTCCAGAGCCATCCCCAGCACT
TGTATTCTGCTAGCTCACTCCGGTCAGCGTCCAGCAGGTGGGTTACGCTCTGCATTTAGCTGGGGCTTCT
AGAACTTCTCTGCAGTGATGTGGTTTCCTCAGAGTTTATCAGTCTGAGTGATTGCATTAGTACAAAGAAG
CTAGCAGACCCAACTGGCCTTTTCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 87761. Forward Primer - name:087761_F_cDNA_AI850995, sequence:GGGATAGAACCCCTAGCTCTGT; Reverse Primer - name:087761_N_SP6_cDNA_AI850995, sequence:GAGAAAAGGCCAGTTGGGTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7565 same embryo
 EMAGE:7563 same embryo
 EMAGE:7564 same embryo
 EMAGE:7561 same embryo
 EMAGE:7560 same embryo
 EurExpress:euxassay_007665 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS