Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7563

Actn3 actinin alpha 3 ( MGI:99678)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7563 EMAGE:7563 EMAGE:7563 EMAGE:7563 EMAGE:7563
"Pseudo-wholemount" of euxassay_007641. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007641_01 euxassay_007641_02 euxassay_007641_03 euxassay_007641_04
EMAGE:7563 EMAGE:7563 EMAGE:7563 EMAGE:7563 EMAGE:7563
euxassay_007641_05 euxassay_007641_06 euxassay_007641_07 euxassay_007641_08 euxassay_007641_09
EMAGE:7563 EMAGE:7563 EMAGE:7563 EMAGE:7563 EMAGE:7563
euxassay_007641_10 euxassay_007641_11 euxassay_007641_12 euxassay_007641_13 euxassay_007641_14
EMAGE:7563 EMAGE:7563 EMAGE:7563 EMAGE:7563 EMAGE:7563
euxassay_007641_15 euxassay_007641_16 euxassay_007641_17 euxassay_007641_18 euxassay_007641_19
EMAGE:7563 EMAGE:7563 EMAGE:7563 EMAGE:7563
euxassay_007641_20 euxassay_007641_21 euxassay_007641_22 euxassay_007641_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7563Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7563_wholemount_strong.wlz
7563_wholemount_moderate.wlz
7563_wholemount_weak.wlz
7563_wholemount_possible.wlz
7563_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7563_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hand
weak weak
regionalweak expression: see section 01 02 22
foot
moderate moderate
regionalmoderate expression: see section 05 06 23
vertebral axis musculature
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
tongue muscle
weak weak
regionalweak expression: see section 08 09 10 11 12 13
tail mesenchyme
weak weak
regionalweak expression: see section 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35875
Entity Detected:Actn3, actinin alpha 3 ( MGI:99678)
Sequence:sense strand is shown

>T35875
GGAGCAGGTAGAGAAGGGCTATGAGGATTGGCTGCTTTCGGAGATCAGGCGTCTGCAGAGGCTTCAGCAC
CTGGCTGAGAAGTTCCAACAGAAGGCTTCCCTGCATGAAGCTTGGACCCGGGGCAAAGAGGAAATGTTAA
ACCAGCACGACTACGAGTCAGCCTCGCTGCAGGAGGTGCGTGCGCTCTTGCGACGTCATGAGGCCTTTGA
GAGCGACCTGGCTGCGCACCAAGACCGGGTGGAACACATCGCGGCCCTGGCCCAGGAACTCAATGAGCTC
GACTACCATGAGGCAGCCTCCGTGAACAGCCGCTGCCAAGCCATCTGTGACCAGTGGGATAACTTGGGTA
CACTGACCCAGAAGAGGAGGGACGCACTAGAGAGGATGGAGAAGCTCCTGGAGACCATTGACCAGCTGCA
GCTGGAGTTTGCTCGGCGGGCAGCCCCCTTCAATAACTGGCTGGATGGGGCTATTGAGGACCTGCAGGAT
GTGTGGCTCGTGCACTCTGTGGAGGAGACCCAGAGCCTGCTAACAGCACACGAACAGTTCAAGGCAACGT
TGCCCGAGGCTGATCGAGAGCGAGGCGCCATCCTGGGCATCCAAGGAGAGATCCAGAAGATTTGCCAAAC
ATACGGACTACGGCCAAAGTCCGGCAACCCCTACATCACCCTCAGTTCGCAGGACATCAACAATAAGTGG
GATACAGTCAGAAAGCTGGTACCCAGCCGTGACCAGACACTGCAGGAGGAGCTGGCCCGGCAGCAGGTGA
ACGAGAGGCTCCGGCGACAGTTTGCAGCCCAGGCTAATGCCATAGGACCCTGGATC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74642. Forward Primer - name:074642_F_cDNA_Actn3, sequence:GGAGCAGGTAGAGAAGGGCTAT; Reverse Primer - name:074642_N_SP6_cDNA_Actn3, sequence:GATCCAGGGTCCTATGGCATT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7565 same embryo
 EMAGE:7564 same embryo
 EMAGE:7561 same embryo
 EMAGE:7560 same embryo
 EMAGE:7562 same embryo
 EurExpress:euxassay_007641 same experiment
 MGI:4822958 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS