EMAGE mouse gene expression spatial database

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EMAGE:7596

Smarcc1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 ( MGI:1203524)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004705. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004705_01	euxassay_004705_02	euxassay_004705_03	euxassay_004705_04

euxassay_004705_05	euxassay_004705_06	euxassay_004705_07	euxassay_004705_08	euxassay_004705_09

euxassay_004705_10	euxassay_004705_11	euxassay_004705_12	euxassay_004705_13	euxassay_004705_14

euxassay_004705_15	euxassay_004705_16	euxassay_004705_17	euxassay_004705_18	euxassay_004705_19

euxassay_004705_20	euxassay_004705_21	euxassay_004705_22	euxassay_004705_23	euxassay_004705_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7596_wholemount_strong.wlz

7596_wholemount_moderate.wlz

7596_wholemount_weak.wlz

7596_wholemount_possible.wlz

7596_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7596_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8670
Entity Detected:	Smarcc1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 ( MGI:1203524)
Sequence:	sense strand is shown >T8670 GGAAACGCCGCGAGGGCCAGGCTAGGCCGGGCGGTAGACACGACGGACGGTGACTATGGCCGCGACAGCG GGTGGCGGTCCGGGAGCAGCAGCAGGCGCCGTGGGTGCAGGGGGTGCGGCGGCGGCCTCCGGGCTGGCCG TGTACCGGAGGAAGGACGGGGGCCCGGCCAGCAAGTTTTGGGAGAGCCCGGACACGGTGTCCCAGCTAGA TTCGGTGCGAGTCTGGCTGGGCAAGCACTACAAGAAGTATGTTCATGCAGATGCTCCTACCAATAAAACA CTAGCTGGACTGGTGGTGCAGCTTCTACAGTTCCAAGAAGATGCCTTTGGGAAGCATGTCACCAACCCAG CTTTCACCAAACTACCTGCAAAATGTTTCATGGATTTCAAAGCTGGAGGCACCTTGTGTCACATTCTTGG GGCAGCTTACAAGTACAAAAATGAACAGGGCTGGCGGAGATTTGATCTTCAGAACCCATCCCGAATGGAT CGTAACGTTGAAATGTTCATGAACATTGAGAAAACATTGGTACAGAACAACTGTCTGACTAGACCAAACA TCTACCTCATTCCAGACATTGATTTGAAGTTGGCTAACAAGTTGAAAGATATCATCAAACGGCATCAGGG GACATTTACTGATGAGAAGTCAAAAGCTTCCCACCATATTTATCCATATCCTTCCTCACAAGAGGATGAG GAGTGGCTGAGACCAGTGATGAGGAGAGACAAGCAGGTGCTGGTGCACTGGGGTTTCTACCCAGACAGCT ATGACACTTGGGTCCACAGTAATGATGTTGATGCTGAAATTGAAGATGCACCAATCCCAGAAAAGCCCTG GAAGGTTCATGTAAAATGGATTTTGGACACTGACGTTTTCAATGAATGGATGAATGAAGAGGATTATGAA GTGGATGAGAACAGAAAGCCAGTGAGCTTTCGTCAACGAATTTCAACAAAGAATGAAGAGCCAGTCAGAA GTCCAGAAAGGAGAGACAGAAAAGCCTCTGCCAACTCTAGGAAGAGGAAACCTTCCCCTTCTCCTCCTCC TCCCACAGCCACAGAGTCCCGCAAGAAGAGCGGGAAGAAAGGACAAGCTAGCCTTTATGGGAAACGTAGA AGTCAGAAGGAAGAAGATGAGCAAGAAGATCTTACCAAGGACATGGAAGACCCCACACCTGTACCTAACA TAGAGGAAGTGGTTCTCCCTAAGAATGTAAACCCAAAGAAGGACAGTGAAAACACACCCGTTAAAGGAGG CACGGTGGCAGATCTAGATGAGCAGGATGAAGAAGCAGTTACAACAGGAGGAAAGGAAGATGAAGATCCC AGCAAAGGTGATCCAAGTCGCTCAGTTGACCCAGGTGAAGACAACGTGACAGAACAGACCAATCACATCA TTATTCCCAGCTACGCATCCTGGTTTGATTATAATTGTATTCATGTCATTGAACGGCGTGCGCTTCCTGA GTTCTTTAATGGAAAAAACAAATCCAAGACCCCTGAAATATACTTGGCATATCGAAATTTTATGATTGAC ACATACCGTCTAAACCCTCAAGAATATTTAACCAGCACTGCTTGCCGGCGAAACCTGACTGGAGATGTGT GTGCTGTGATGAGGGTTCATGCCTTCTTAGAGCAGTGGGGTCTTGTTAACTACCAAGTTGACCCAGAGAG TCGACCCATGGCAATGGGACCTCCTCCCACTCCTCACTTCAATGTGTTAGCTGACACACCCTCTGGGCTT GTGCCCCTGCATCTTCGATCACCTCAGGTCCCTGCCGCTCAACAGATGTTAAATTTTCCTGAGAAGAACA AGGAAAAACCAATTGATTTGCAGAACTTTGGTCTTCGAACTGACATTTACTCCAAGAAAACACTGGCAAA GAGTAAAGGTGCTAGTGCTGGAAGGGAGTGGACAGAACAGGAGACCCTTCTTCTCCTAGAGGCTCTGGAG ATGTACAAGGACGATTGGAATAAAGTGTCAGAACATGTTGGAAGCCGTACTCAGGACGAATGCATCCTCC ACTTTCTGAGGCTTCCCATTGAGGACCCTTACCTTGAAAATTCAGATGCTTCTCTTGGGCCACTGGCTTA CCAGCCTGTCCCTTTCAGCCAGTCGGGAAACCCGGTGATGAGCACTGTTGCCTTTTTAGCATCTGTCGTT GACCCCCGTGTAGCATCTGCTGCAGCAAAAGCAGCGTTGGAGGAGTTTTCTCGTGTCCGAGAAGAAGTAC CCCTGGAATTGGTTGAAGCACATGTCAAGAAAGTACAGGAAGCTGCAAGAGCCTCTGGGAAGGTGGACCC CACCTATGGCTTGGAGAGCAGCTGTATTGCTGGCACAGGGCCTGACGAGCCAGAGAAGCTTGAAGGATCT GAAGAAGAGAAGATGGAAACAGATCCTGATGGTCAGCAGCCTGAAAAGGCAGAAAACAAAGTGGAAAATG AATCGGATGAAGGTGATAAAATACAAGATCGAGAGAATGAAAAAAACACTGAGAAGGAACAAGATAGTGA CGTCAGTGAGGATGTCAAGCCAGAAGAAAAGGAGAATGAAGAGAACAAAGAGCTCACTGATACATGTAAA GAAAGAGAAAGCGATGCCGGGAAGAAGAAAGTGGAACACGAGATTTCGGAAGGAAACGTTGCCACAGCCG CAGCAGCTGCTCTGGCCTCAGCTGCTACTAAAGCCAAGCACCTGGCGGCTGTTGAAGAAAGAAAAATCAA GTCCTTGGTAGCTCTCTTGGTTGAAACACAAATGAAGAAACTAGAGATCAAACTTCGACATTTTGAAGAG CTGGAGACTATAATGGACAGAGAGAAAGAGGCTCTAGAACAACAGAGACAGCAGTTGCTTACTGAGCGTC AGAACTTCCACATGGAACAGTTGAAATATGCTGAACTACTTGCCCGGCAGCAAATGGAGCAGCAGCAGCA GCATGGCCAGACACCTCAGCAGGCGCACCAGCACACGGGAGGGCCGGGGATGGCCCCACTTGGAGCCACA GGCCACCCTGGCATGATGCCGCATCAGCAGCCCCCTCCCTACCCACTGATGCACCATCAGATGCCGCCAC CCCATCCTCCCCAACCAGGTCAAATACCAGGCCCTGGCTCCATGATGCCTGGCCAGCCCATGCCAGGTCG CATGATCCCCGCTGTGGCAGCCAACATTCACCCTACTGGGAGTGGCCCTACCCCTCCTGGTATGCCTCCA ATGCCCGGAAACATCTTAGGACCCCGGGTACCCCTCACAGCACCAAACGGCATGTGTAAGTAGCACAGTG GGACGACATTACCCAGCATCCTGCTTTGGAAGCTACAGATAAGGAAGAAATGCTTTCTTCGTGAGATTAG CAAGCCCATAGACAGTCTAGCCTGCAACAGTGAGGTGTGGGAGATAACGTTTTGGTGTGAACACTCAGGT TGTGTACTCAGTACTGAGATCTGTGGGGAAGCCACTGATTCTGTCACTCATGATTAAACCTGACTAGTTT GAATCTCATCTGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400646 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400646 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7598 same embryo
	EMAGE:7599 same embryo
	EMAGE:7597 same embryo
	EMAGE:7600 same embryo
	EMAGE:7595 same embryo
	EurExpress:euxassay_004705 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source