EMAGE mouse gene expression spatial database

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EMAGE:7599

Gja5 gap junction protein, alpha 5 ( MGI:95716)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004691. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004691_01	euxassay_004691_02	euxassay_004691_03	euxassay_004691_04

euxassay_004691_05	euxassay_004691_06	euxassay_004691_07	euxassay_004691_08	euxassay_004691_09

euxassay_004691_10	euxassay_004691_11	euxassay_004691_12	euxassay_004691_13	euxassay_004691_14

euxassay_004691_15	euxassay_004691_16	euxassay_004691_17	euxassay_004691_18	euxassay_004691_19

euxassay_004691_20	euxassay_004691_21	euxassay_004691_22	euxassay_004691_23	euxassay_004691_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7599_wholemount_strong.wlz

7599_wholemount_moderate.wlz

7599_wholemount_weak.wlz

7599_wholemount_possible.wlz

7599_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7599_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm mesenchyme

strong

regional

strong expression: see section 01 02

hand mesenchyme

strong

regional

strong expression: see section 03 04 05 23 24

foot mesenchyme

strong

regional

strong expression: see section 04 05 06 07 19 20

lower leg mesenchyme

strong

regional

strong expression: see section 01 02 03 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

heart atrium

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 16 17 18 19 20 21 22

heart ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 16 17

tongue muscle

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 11 12 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8679
Entity Detected:	Gja5, gap junction protein, alpha 5 ( MGI:95716)
Sequence:	sense strand is shown >T8679 ATAACAGTGGGCAGTTGAACAGCAGCCAGAGCCTGAAGAAGCCAACTCCAGGGAGGAGGAAAGGAAGCAG AAGGCTCGGCCTCGGTCTCCTACTCTTGGCAAGATGGGTGACTGGAGCTTCCTGGGGGAGTTCCTGGAGG AGGTCCACAAGCACTCCACAGTCATCGGCAAGGTCTGGCTCACTGTCCTGTTCATTTTCCGCATGCTGGT CCTGGGCACCGCTGCTGAGTCCTCCTGGGGAGATGAGCAGGCCGACTTCCGGTGCGATACCATTCAGCCT GGTTGCCAAAATGTCTGCTATGACCAAGCCTTCCCCATCTCCCACATTCGTTATTGGGTACTGCAGATCA TCTTTGTGTCCACGCCTTCTCTAGTGTACATGGGCCATGCCATGCACACTGTGCGCATGCAGGAAAAGCA GAAATTGCGGGATGCTGAGAAAGCTAAAGAGGCCCACCGCACTGGTGCCTATGAGTACCCAGTAGCCGAA AAGGCCGAGCTGTCCTGCTGGAAAGAAGTAGATGGGAAGATTGTCCTCCAGGGCACCCTACTCAACACCT ATGTCTGCACCATTCTGATCCGCACCACCATGGAGGTGGCCTTCATCGTAGGCCAGTACCTCCTCTATGG GATCTTCCTGGATACCCTGCATGTCTGCCGCAGGAGTCCCTGTCCCCACCCAGTCAACTGTTATGTTTCG AGGCCCACGGAGAAGAATGTCTTCATTGTCTTTATGATGGCTGTGGCTGGACTGTCTCTGTTTCTCAGCC TGGCTGAACTCTACCACCTGGGCTGGAAGAAGATCCGACAGCGCTTTGGCAAGTCACGGCAGGGTGTGGA CAAGCACCAGCTGCCTGGCCCTCCCACCAGCCTCGTCCAGAGCCTCACTCCTCCCCCTGACTTCAATCAG TGCCTAAAGAACAGCTCCGGAGAGAAATTCTTCAGCGACTTCAGTAATAACATGGGCTCCCGGAAGAATC CAGACGCTCTGGCCACTGGGGAAGTGCCAAACCAGGAGCAGATTCCAGGGGAAGGCTTCATCCACATGCA CTATAGCCAGAAGCCAGAGTACGCCAGTGGAGCCTCTGCGGGCCACCGCCTTCCTCAGGGCTACCATAGT GACAAACGGCGCCTTAGTAAGGCCAGCAGCAAAGCAAGGTCAGATGACCTGTCAGTGTGATCCTCCTTTT AGGGAGGGCCAGGCCCAGGAGGGATCTAAGGAGTGTGAGGCAGGAAAGAAACAGTCTCTCTCGTGATGGT ATACTCTCCTCAGCACTACTTTGCTCTGTCTGTCCTGGGTCTGGGTGGCATTCATTGGCATTCATTGCTC GTTATCCTCAAAATTACAGTTCTGAGTCTTCTCAATGACTGGGCTACTCCTCAAGTCTCATTCCCTCCTA CTCATCTACCAGTATTCACAGTGAAGCCATAGTGGAGGCAGGATCAGGAACCGTGGCAAAATCTGGTCTG GGGAGGACAGGTTCAACACCAGCAACATACCAGATAGACCCCCCACCCACCCCAAGTCTTCTCTTTGGTA CTATGCCCATCCTCTTGTTTTGTTTTGTTTTGTTTTTTCTTAAGAGCCCAAGGTTGAGAGTATTCATCAA GTAATCTGCCCTGTATCATTCCTTTGAATCAGTTGTTTCCATGTGCCATACCTCGGAGTGCTGGTGAGGT AACTGTGGGCTGCCCTTGGCTGCATCTCCTTCCTCCCAGTCTTAAAGGGGTATCCTTGGTCCTTGGCCAG CATCAGATTTACAAGTGCCTTGGTATGTACCTTTGGCAAATGTTTCATGTTGATGATGTCTCAGCCTTAC TGCCAGGCTAGACACCCAGCTCGTAAGGGTGCCAAGACCTCCAGAAGTCACTGTGCACACAAGCATCGCT GGAATTGCCACCAGCTGTCATCCTGCGGTCCTTTATAGTTTAACCTAATGACAGAAACCATCCTGGCCGG CTCACCCACCGTTCCTCTAAAATCTTTGTTAACAGGAATGTCCAAAGTCACCATCCTCTCTTGGATCCTG GCCAGATTACCAGCCACCAAGTTCAGTGGAATTTCCCCAGGATTTACTGAAACAAAGAGAGCATTGTGCT TCTCGGGTTCTTCTGGGTCTGGGGATGGGGGTGGAGGGGGAAATTCTGCTGTGAAGATGAAAAGGTGGGG GGAGGACACTGAAAAACTATTTTCCCTTCTGTGTATGCCTTCCACTGTCTGCCAGCTTTTAATGCCGAGA GGTGTGGTGATAGCAATTGAGGCCCAAGATCTCTCTTAGAGGCATCAAGGAGACATGGAAGCAGAAAGGG TCCAGTTGGTTTGGACCCTTATTTAGTGCAGATATTCCTATGTTTTCTTGGTACCCCTAGGTACAACAGG GGAGGACTGTAGAAAATCCCTGCCTTCCTCAGGGTCTATCCTGGGGTATCTACTGAGTGGATCCATCTTA TGCAGATGGAAGAAGCTCAGCACCAGATACCGAGATTTACAAACACAATACTGTGTGTGTGTGTGTGTGT GTGTGTGTGTGTGTGTGTTTGTGTGTGCATGCCCATCCATCTCTGTGTGCCTCTGTGTGTGTGTGCTCGT GTGTGCATGCAGATAGAATGGTGAAGAAGAGCCCTGAAGAACGATAAGGAAACATGTAGAGAAACTTGGG TCTTTCTGCTTCCTTCTCAGTTGTCTGGCAGGAGGGCTGAGCTTGCTTCTTAATCAGCCTAAAGATCAGG CATGAGAAGTTCAGGAGGGTGAAGGGTCAGAGGTCAGTCCCTGTCCCAGTGACCCATCTCTGGGTCCCTC TGCTGGCATTTGGTTGCTACTAATGGATAGAAAGAAATTGACTGAGGATACCTGGGAAAGGAAGACGGGC TGTTCCAGTCCCAGTGCATTTCATCCATTCCCGACACTGGCACTAAGACTCTCTGTTCTGGCTATGGAGT GTGTGTTTGTTTTCTGAAATGGGATTTTTTTGAATTAGAAAATCTGAGACCAGTCGTTGATGGTGAGGTT TTTTTTGTTTTCTGTTTTTTTTTTTTTTTTTTTTTCAGAAGAGAAAGAGGAAATACTATGTTTGACTGGT TTTAAATAAAATCTATCTCTACATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6401526 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401526 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7598 same embryo
	EMAGE:7597 same embryo
	EMAGE:7600 same embryo
	EMAGE:7595 same embryo
	EMAGE:7596 same embryo
	EurExpress:euxassay_004691 same experiment
	MGI:4825057 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source