EMAGE mouse gene expression spatial database

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EMAGE:7603

Ripk1 receptor (TNFRSF)-interacting serine-threonine kinase 1 ( MGI:108212)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004703. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004703_02	euxassay_004703_03	euxassay_004703_04	euxassay_004703_05

euxassay_004703_06	euxassay_004703_07	euxassay_004703_08	euxassay_004703_09	euxassay_004703_10

euxassay_004703_11	euxassay_004703_12	euxassay_004703_13	euxassay_004703_14	euxassay_004703_15

euxassay_004703_16	euxassay_004703_17	euxassay_004703_18	euxassay_004703_19	euxassay_004703_20

euxassay_004703_21	euxassay_004703_22	euxassay_004703_23	euxassay_004703_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7603_wholemount_strong.wlz

7603_wholemount_moderate.wlz

7603_wholemount_weak.wlz

7603_wholemount_possible.wlz

7603_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7603_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8649
Entity Detected:	Ripk1, receptor (TNFRSF)-interacting serine-threonine kinase 1 ( MGI:108212)
Sequence:	sense strand is shown >T8649 CGAAAAGCGCGGAACTTGCTGTCATCTAGCGGGAGGTTGGACTCTTCTTGAGGTCGTTTTAGCTCAAGTC GAGACTGAAGGACACAGCACTAAGCAAGAACCAAAAGTGGTGTGTTGGAGATTCTGAGCAATCAAAATGC AACCAGACATGTCCTTGGACAATATTAAGATGGCATCCAGTGACCTGCTGGAGAAGACAGACCTAGACAG CGGAGGCTTCGGGAAGGTGTCCTTGTGTTACCACAGAAGCCATGGATTTGTCATCCTGAAAAAAGTATAC ACAGGGCCCAACCGCGCTGAGTACAATGAGGTTCTCTTGGAAGAGGGGAAGATGATGCACAGACTGAGAC ACAGTCGAGTGGTGAAGCTACTGGGCATCATCATAGAAGAAGGGAACTATTCGCTGGTGATGGAGTACAT GGAGAAGGGCAACCTGATGCACGTGCTAAAGACCCAGATAGATGTCCCACTTTCATTGAAAGGAAGGATA ATCGTGGAGGCCATAGAAGGCATGTGCTACTTACATGACAAAGGTGTGATACACAAGGACCTGAAGCCTG AGAATATCCTCGTTGATCGTGACTTTCACATTAAGATAGCCGATCTTGGTGTGGCTTCCTTTAAGACATG GAGCAAACTGACTAAGGAGAAAGACAACAAGCAGAAAGAAGTGAGCAGCACCACTAAGAAGAACAATGGT GGTACCCTTTACTACATGGCACCCGAACACCTGAATGACATCAATGCAAAGCCCACGGAGAAGTCGGACG TGTACAGCTTTGGCATTGTCCTTTGGGCAATATTTGCAAAAAAGGAGCCCTATGAGAATGTCATCTGTAC TGAGCAGTTCGTGATCTGCATAAAATCTGGGAACAGGCCAAATGTAGAGGAAATCCTTGAGTACTGTCCA AGGGAGATCATCAGCCTCATGGAGCGGTGCTGGCAGGCGATCCCAGAAGACAGGCCAACATTTCTTGGCA TTGAAGAAGAATTTAGGCCTTTTTACTTAAGTCATTTTGAAGAATATGTAGAAGAGGATGTGGCAAGTTT AAAGAAAGAGTATCCAGATCAAAGCCCAGTGCTGCAGAGAATGTTTTCACTGCAGCATGACTGTGTACCC TTACCTCCGAGCAGGTCAAATTCAGAACAACCTGGATCGCTGCACAGTTCCCAGGGGCTCCAGATGGGTC CTGTGGAGGAGTCCTGGTTTTCTTCCTCCCCAGAGTACCCACAGGACGAGAATGATCGCAGTGTGCAGGC TAAGCTGCAAGAGGAAGCCAGCTATCATGCTTTTGGAATATTTGCAGAGAAACAGACAAAACCGCAGCCA AGGCAGAATGAGGCTTACAACAGAGAGGAGGAAAGGAAACGAAGGGTCTCTCATGACCCCTTTGCACAGC AGAGAGCTCGTGAGAATATTAAGAGTGCAGGAGCAAGAGGTCATTCTGATCCCAGCACAACGAGTCGTGG AATTGCAGTGCAACAGCTGTCATGGCCAGCCACCCAAACAGTTTGGAACAATGGATTGTATAATCAGCAT GGATTTGGAACTACAGGTACAGGAGTTTGGTATCCGCCAAATCTAAGCCAAATGTATAGTACTTATAAAA CTCCAGTGCCAGAGACCAACATACCGGGAAGCACACCCACCATGCCATACTTCTCTGGGCCAGTAGCAGA TGACCTCATAAAATATACTATATTCAATAGTTCTGGTATTCAGATTGGAAACCACAATTATATGGATGTT GGACTGAATTCACAACCACCAAACAATACTTGCAAAGAAGAGTCGACTTCCAGACACCAAGCCATCTTTG ATAACACCACTAGTCTGACTGATGAACACCTGAACCCTATCAGGGAAAACCTGGGAAGGCAGTGGAAAAA CTGTGCCCGCAAGCTGGGCTTCACTGAGTCTCAGATCGATGAAATCGACCATGACTATGAAAGAGATGGA CTGAAAGAGAAAGTTTACCAAATGCTTCAGAAGTGGCTGATGCGGGAAGGCACCAAAGGGGCCACAGTGG GAAAGTTGGCCCAGGCACTTCACCAATGTTGCAGGATAGACCTGCTGAACCACTTGATTCGTGCCAGCCA GAGCTAAGCCTGGGCAGGCTCTGGCAGTGGGAAGCAAACTATTTGTCTGGTGCACAAACCCCGTTTGCCC ACTAGCCTTCAGAACTCTATCTCAGCATGAGCTCTGCATTTGAGCACACAGGGTCATGCAGTTTGGAACT GGTGGATGGGAAGAGAAATCTGAAGCCCACAGTGATTCTTCAGAACATGCAAGCATAAAGACCGCTGAAT GAATGGTCGGTCCATGACCAGTAGGAGGAAAAAAATTAAAAATACAGTGTATTGAGTTTTCAAAGGGAGA GAAGATGTTGGGGGAAGGTGGCCTTCGTTCAGCTTGTGTCATAGTCATCACTTAGGTTATTTGCTCAGTT TCCTGTGGTTTCATTGGGTAAGGGGAAAACAAACAAAAAACATTCAAAGAATGTAAAATCAGCTAGCTTC CCGCTTCATGGACTGTGAGTCAGAAAAGTTCTGCTCAAATGCCTTCTATGAGCCACGGCTCTTCTAAAAA CTATAGGATCTGTTAACTTGAGCATCCAATGCTGTACTGTCACCTTAAGCTTTAAGAATCATGTGTAATT CTAGTCTATCAAATTATCAATTAGTGAGAGATTTTTAAGGAGTGCTAAGGAGAAAAAGGTGCAGGGCAGA GAGAGATCTTCAGGTACTGGCTGTCATTGCAGCTCAGGTACTGGCTGTCATTGCAGAGCTGCTGCTGGTT CTGGCTGCTGTAAAAAGTCTACTTTTAAGTTTCTAAAGTTTCTCTACCATTCTGGCGCTTCATGCAGTTT CCCACCCTGTGAGCCCCACAGAGGGAACAGATGTGCAGGAAACACTGATCGGGAAATACCATCTCCTCCT CTCTGGATATGACTGGTGCCTTAGCACTGTTCCCTGTGCCCAATAAACTGTTTCCTCAGTTTTCTCCAGT GTGCTGTACAAATATGTTTCTTCTGTGTGCCACCAATCAAGAAAGGACAGCTTCAGAGTCATCTTGTGCC TTTAGGAAGCCCATATTGCAACCTACAAGTGAGTGAAGGAGCTAGGGTAGAAGACTGAAGCCGCCTACAC CAGCCACAGTCACTGTTAGGATGGGCTGCACAGCCGCTTTGGCCTTGTTGGCCATTCTGGCACTCATTGG CACTTCATCCTCCTTTGTTGGGCTATCCTGTACTCAGTAGGATATTTGGGAACATTCCTGGCCTCCATCT GCAAGATGCCAACAGTACAAGTCAACCTCACAACACCCACCAGGTGTGAGAAAAATAACTAGATATTGCT ACATGTTCTTTGGAGGGACTCTCCTGTAACGTTATTTCCCCCATCCCCAGACAAGGTTTCTCAGTTCTCG CTGTCCTGGAACTCACTTTGTAGACCAGATTGGTCTCCCAATTCAGAGATCTGCCTGCCTCTGCCTCCTG AGTGCTGGGAATAAAGGCATGTGCCACCACCACCCAGCTTCCTGCTGTAAGTTTATATGCCTTACTTCTC CTGTCACACAGGCTAGGCCATCATTAACAGCATGGAACATGCACAGTGGAAGGGAATTGGAGCAGGGAAG GTTCCAGGCTTTCCTGAAGGAGCCTGCATAGTCTTTAAAAAATGTTTTCTTTTTAACTTCTAATGCTGTT TTAAGAAGACAGAATTAGAAACTGCTTCTAGTGGAGTTTTAAGGAGAATAAAATAGCAAGGGAGGCCAGA GCTGGTTTTGGAATTGCAGTAGCAATCAGGGTCCTAGTATTAAAATCATGTCTGTTAGTGTTCAGTAGGG GAAGACTTGTGTGTCCACTGCTGAAGATAGGTGCTTCAGATCCAGGCCTGGATTGTGACCACAGACTTTC AGATCTTTTCCAGGCTCACCACTGACAGCCAGACAATGGCTTGGCACCTCGAGTTGTGGCTGAGCCTGTA TGAGTTGAAGGATGGTCAGTAGATGAAGCCGTTTACTCTCATGGTGTGTGCCCTTGTACAAGAGATCTGG GCGCGTGACACAAAGCCAGTCATTGACAGGCTAGACCCAAAGACCTTTAAGCACTCCCATTTGCTTCCGC TGTATCACAATAACTGATGACTTTGTAGTCAGTGGTACCTGCTCCGAACAAGTACGTGGAGTGTGGGAGT CCCTCTGGGAGCCCAGCGTGGAATTAGAACACCTCTTTAAAGCCACCTCCTAAGCCGAGCAGAATGCAGT GGACTGGGGTACAAGTCAGACCTGAGTGTCATTGTCCACATCATGGAAAAACAAGATGGCCACCAGAACA CTGAAGGCCTGAGGAGACTAACCCTGTTCCCAGAACTCAGAGTTCTGTTTGGTTCTGTTTTTAATAAGAT AACCTTTCTTTAATGTATACATATGCATATACATATATATTTGTACTTTAAATACAGAGTACTGAATAAA ATTTATGTGACTATAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5721177 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5721177 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7601 same embryo
	EMAGE:7604 same embryo
	EMAGE:7606 same embryo
	EMAGE:7605 same embryo
	EMAGE:7602 same embryo
	EurExpress:euxassay_004703 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source