EMAGE mouse gene expression spatial database

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EMAGE:7604

Hdac5 histone deacetylase 5 ( MGI:1333784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004693. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004693_01	euxassay_004693_02	euxassay_004693_03	euxassay_004693_04

euxassay_004693_05	euxassay_004693_06	euxassay_004693_07	euxassay_004693_08	euxassay_004693_09

euxassay_004693_10	euxassay_004693_11	euxassay_004693_12	euxassay_004693_13	euxassay_004693_14

euxassay_004693_15	euxassay_004693_16	euxassay_004693_17	euxassay_004693_18	euxassay_004693_19

euxassay_004693_20	euxassay_004693_21	euxassay_004693_22	euxassay_004693_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7604_wholemount_strong.wlz

7604_wholemount_moderate.wlz

7604_wholemount_weak.wlz

7604_wholemount_possible.wlz

7604_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7604_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

moderate

homogeneous

moderate expression: see section 04 05 09 10 11 12 13 15 16 17 18 19 20 21 22 23 weak expression: see section 06 07 08

not examined

regional

not examined expression: see section 04

olfactory cortex marginal layer

moderate

homogeneous

moderate expression: see section 10 11 12 13 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8639
Entity Detected:	Hdac5, histone deacetylase 5 ( MGI:1333784)
Sequence:	sense strand is shown >T8639 CGAAGGATGGCCTTGGATGGGCATTAGAGGCACTGCGGCCCTGGGCTCGGGCTCCTGTCCCGTCCGTCTG TCTGTTATCGTCTGTCTCTCTTGACATCACAGCAGCTCCGCCCCCTCCCGTCCCAGCCCCCAACGTCAGC TTCCTGCAGGCCCAGAGCCGGCATGAACTCTCCCAACGAGTCGGATGGCATGTCAGGCCGGGAACCATCC TTGGAAATCCTGCCACGGACTCCTCTGCACAGCATCCCTGTGGCAGTGGAGGTGAAGCCGGTGCTGCCAG GAGCCATGCCCAGCTCCATGGGGGGTGGAGGTGGAGGTAGCCCCAGCCCCGTGGAGCTTCGGGGGGCTCT GGCGGGCCCCATGGACCCTGCGCTACGGGAGCAGCAACTGCAGCAGGAGCTCCTGGTCCTCAAGCAGCAG CAGCAGCTCCAGAAGCAGCTCCTGTTCGCCGAGTTCCAGAAGCAGCACGACCACTTGACGCGGCAGCACG AGGTCCAGCTGCAGAAGCACCTCAAGCAGCAGCAGGAGATGCTGGCGGCTAAGAGGCAGCAGGAGCTGGA GCAGCAGCGGCAGCGGGAGCAGCAGCGGCAGGAGGAGCTGGAGAAACAGCGGCTGGAGCAGCAGCTGCTC ATCCTGCGCAACAAGGAGAAGAGCAAAGAGAGTGCCATCGCCAGCACCGAGGTAAAGCTGAGGCTCCAGG AATTCCTGTTGTCCAAGTCAAAGGAGCCCACGCCAGGCGGCCTCAACCATTCCCTCCCACAGCACCCCAA ATGCTGGGGAGCCCACCACGCTTCTTTGGACCAGAGTTCCCCTCCCCAGAGCGGCCCTCCTGGGACGCCT CCCTCCTACAAATTGCCTTTGCTTGGGCCCTATGACAGCCGTGATGACTTTCCCCTCCGTAAAACGGCCT CGGAACCCAACTTAAAAGTACGTTCGAGGCTAAAACAGAAGGTAGCCGAGAGGAGAAGCAGTCCCCTCCT GCGTCGAAAGGATGGCACTGTTATTAGTACTTTTAAGAAGAGAGCAGTTGAGATCACCGGCACGGGGCCT GGGGTGTCGTCCGTGTGTAACAGTGCGCCCGGCTCTGGCCCCAGCTCTCCCAACAGTTCCCACAGCACCA TCGCTGAGAACGGCTTTACTGGCTCAGTCCCCAACATCCCCACTGAGATGATCCCCCAGCACCGGGCCCT CCCTCTGGACAGTTCCCCAAACCAGTTCAGCCTCTATACGTCTCCTTCTCTGCCCAACATCTCCCTAGGG CTGCAGGCCACTGTCACTGTCACCAACTCGCACCTCACCGCCTCCCCGAAGCTGTCAACACAGCAGGAGG CTGAGAGGCAGGCCCTTCAGTCCCTGCGGCAGGGCGGCACACTGACCGGCAAGTTCATGAGCACATCCTC CATCCCTGGCTGCCTGTTGGGAGTGGCACTGGAGGGTGACACAAGCCCCCACGGGCACGCTTCCCTGCTG CAGCACGTTTTGCTCCTGGAGCAGGCCCGGCAACAGAGCACGCTCATAGCAGTGCCGCTCCATGGGCAGT CCCCACTGGTGACGGGTGAACGTGTGGCCACCAGCATGAGGACGGTGGGTAAGCTCCCGAGGCACCGACC TCTGAGCCGCACTCAGTCCTCCCCGCTGCCGCAGAGTCCCCAGGCCCTGCAGCAGCTGGTCATGCAGCAG CAGCACCAGCAGTTCCTGGAGAAGCAGAAGCAGCAGCAGATGCAGCTGGGCAAGATCCTTACCAAAACTG GGGAGCTGTCAAGGCAGCCCACCACTCACCCGGAGGAGACAGAAGAGGAGCTGACGGAGCAGCAGGAGGC CTTGCTGGGAGAGGGGGCCCTGACCATTCCCCGGGAAGGCTCTACAGAAAGTGAGAGCACCCAGGAAGAC CTAGAAGAGGAGGAGGAGGAGGAGGAGGAGGAAGAGGAGGACTGCATTCAGGTCAAGGATGAGGATGGCG AGAGTGGTCCTGATGAAGGCCCTGACTTAGAAGAGTCCAGTGCTGGTTACAAAAAGTTGTTCGCAGATGC CCAGCAGTTACAGCCCCTCCAGGTGTACCAGGCACCCCTCAGCCTGGCCACTGTGCCTCATCAGGCCCTG GGCCGCACCCAGTCCTCACCTGCTGCTCCTGGGAGCATGAAGAGCCCCACAGACCAACCCACTGTGGTGA AGCACCTCTTCACCACAGGTGTGGTCTATGACACGTTCATGCTGAAGCACCAGTGTATGTGCGGAAACAC ACACGTGCACCCAGAGCACGCCGGCCGCATCCAGAGCATCTGGTCCCGGCTGCAGGAAACTGGTCTGCTC GGCAAGTGTGAGCGGATCCGGGGTCGTAAAGCCACACTGGATGAAATCCAGACCGTGCACTCTGAGTACC ACACCCTGCTCTATGGGACCAGCCCCCTTAACCGGCAGAAGCTGGACAGCAAGAAGCTGCTTGGCCCCAT CAGCCAGAAGATGTACGCCATGCTGCCCTGTGGGGGCATTGGGGTGGACAGTGACACGGTGTGGAATGAG ATGCACTCCTCAAGTGCCGTGCGAATGGCAGTGGGCTGCCTGGTGGAGCTGGCCTTCAAGGTGGCTGCAG GAGAGCTCAAGAATGGATTTGCTATCATCCGGCCCCCAGGACACCATGCTGAGGAGTCCACAGCCATGGG ATTCTGCTTCTTCAACTCCGTAGCCATCACAGCTAAACTCCTGCAGCAGAAGCTGAGCGTGGGCAAGGTC CTCATCGTGGACTGGGATATTCACCATGGCAACGGCACCCAGCAAGCATTCTACAACGATCCCTCTGTGC TCTACATCTCCCTGCATCGCTACGACAACGGGAACTTCTTTCCAGGCTCTGGGGCTCCTGAAGAGGTTGG TGGAGGGCCAGGTGTGGGGTACAACGTAAATGTGGCGTGGACAGGAGGTGTGGATCCCCCCATTGGAGAT GTGGAATACCTGACAGCCTTCAGGACAGTGGTGATGCCCATTGCCCAGGAGTTCTCACCTGACGTCGTCC TAGTCTCCGCTGGGTTTGATGCTGTTGAAGGACATCTGTCTCCACTGGGTGGCTATTCTGTCACCGCCAG ATGTTTTGGCCACTTGACCAGGCAGCTCATGACACTGGCTGGGGGCCGGGTGGTGCTGGCCCTGGAGGGA GGCCATGACTTGACCGCCATCTGTGATGCCTCTGAGGCCTGTGTCTCGGCTCTGCTCAGCGTGGAGCTGC AGCCCTTGGATGAAGCAGTCTTGCAGCAAAAGCCCAGCGTCAATGCGGTTGCCACACTAGAGAAAGTCAT CGAGATCCAGAGCAAACACTGGAGCTGTGTACAGAGGTTTGCCGCTGGTCTGGGCTGCTCGCTGCGGGAG GCTCAGACAGGTGAGAAAGAGGAGGCCGAGACTGTGAGCGCCATGGCCCTGCTTTCCGTGGGGGCTGAGC AGGCCCAGGCTGTTGCCACTCAAGAGCACAGCCCCAGGCCAGCAGAGGAGCCCATGGAGCAGGAGCCTGC CCTGTGACACCCTGGCCCCCATCCCTCTGGGCTTCATCATTGTGATTTTGTTTATTTTTTCTATTAAAAA CAAAAAGTCACACATTCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5720438 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5720438 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7601 same embryo
	EMAGE:7606 same embryo
	EMAGE:7603 same embryo
	EMAGE:7605 same embryo
	EMAGE:7602 same embryo
	EurExpress:euxassay_004693 same experiment
	MGI:4825341 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source