EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7605

Smap2 stromal membrane-associated GTPase-activating protein 2 ( MGI:1917030)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004704. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004704_01	euxassay_004704_02	euxassay_004704_03	euxassay_004704_04

euxassay_004704_05	euxassay_004704_06	euxassay_004704_07	euxassay_004704_08	euxassay_004704_09

euxassay_004704_10	euxassay_004704_11	euxassay_004704_12	euxassay_004704_13	euxassay_004704_14

euxassay_004704_15	euxassay_004704_16	euxassay_004704_17	euxassay_004704_18	euxassay_004704_19

euxassay_004704_20	euxassay_004704_21	euxassay_004704_22	euxassay_004704_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7605_wholemount_strong.wlz

7605_wholemount_moderate.wlz

7605_wholemount_weak.wlz

7605_wholemount_possible.wlz

7605_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7605_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08

trigeminal v ganglion

moderate

regional

moderate expression: see section 22 weak expression: see section 04 05 06 07 08 09 19 20 21

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 09 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8637
Entity Detected:	Smap2, stromal membrane-associated GTPase-activating protein 2 ( MGI:1917030)
Sequence:	sense strand is shown >T8637 AGAGCTGCCGGAAGTAGGCGGTGGAGGTGGCGGCTGAGCTGGTCGCAGCTGCCAGGGAAACCGAGGCGGG AAGCACAGCGGGCAGACCCTCAACACTCGGGGTCAAGTGTGCGTCCCGCACGCCTGAAGCCGGGAGCTCG CTCCACCTAGGCCACTTCTGGGGTCCCGCAGCCTCCGCGAGTCCCTGCCTTGCCCGAAGCCCGCCAGGGG GCGCCGCGCTGAGGGGCTGAGGGGCTGCGGGGCGGGGGCGCTGACGGCTCTGACCCCGGGGCTGCTCCGG GCCGGGCGAGAGGACCGCCTGGGCGGGGACCCCAAGGCCTCAGCCCCGGACAGAGGCAGGGGTTCTTGGG GGGTGCAGGGGGCGCGTCGCCCTCTGCCCCCGCCGGCACCCTGGCCATGACAGGCAAGTCGGTGAAGGAC GTGGATCGGTACCAGGCGGTCCTGGCCAACCTGCTGCTGGAGGAAGATAACAAGTTCTGTGCTGACTGCC AGTCCAAAGGGCCGAGATGGGCCTCCTGGAACATCGGGGTGTTCATCTGCATTCGGTGTGCTGGGATCCA CAGGAATTTGGGGGTGCATATATCCAGGGTAAAATCAGTGAACCTCGACCAGTGGACTCAAGAACAGATT CAGTGCATGCAAGAGATGGGGAATGGAAAAGCAAACCGACTCTATGAAGCCTACCTTCCCGAGACCTTTC GGCGACCTCAGATAGACCCAGCTGTTGAAGGATTTATTCGAGATAAATATGAGAAGAAGAAATATATGGA CCGAAGTCTGGACATCAATGTCCTTAGGAAAGAGAAGGATGATAAGTGGAAACGAGGGAATGAACCCGCT CCGGAGAAAAAGATGGAACCTGTTGTTTTTGAGAAAGTAAAAATGCCACAGAAAAAAGAAGACGCACAGC TACCTCGGAAAAGCTCTCCGAAATCTGCAGCCCCTGTCATGGACTTGTTGGGCCTTGATGCTCCTGTGGC CTGCTCAATTGCAAACAGTAAGACCAGCAATGCCCTAGAAAAGGATCTAGATCTTTTGGCCTCTGTTCCG TCCCCTTCTTCAGTTTCCAGAAAGGCTGTAGGTTCCATGCCAACTGCAGGGAGTGCTGGTTCTGTCCCTG AAAACCTGAACCTATTTCCAGAGCCAGGGAGCAAATCAGAAGAAACAGGCAAGAAACAGCTCTCCAAGGA CTCCATCCTATCACTGTATGGATCCCAGACGCCTCAAATGCCTGCCCAAGCAATGTTCATGGCTCCTGCT CAGATGGCATACCCCACAGCTTACCCCAGTTTCCCCGGGGTTACACCACCTAACAGCATCATGGGGGGCA TGGTGCCTCCGCCAGTAGGCATGGTAGCTCAACCGGGAGCCTCTGGAATGCTAACCCCTATGGCCATGCC TGCAGGCTATATGGGGGGCATGCAGGCGTCCATGATGGGTGTGCCGAACGGGATGATGACCACTCAGCAG GCCGGCTACATGGCAAGCATGGCAGCTATGCCGCAGACTGTGTATGGAGTTCAGCCAGCTCAGCAACTGC AATGGAACCTTACTCAGATGACCCAGCAAATGGCTGGGATGAACTTCTACGGAGCCAACGGCATGATGAA CTATGGACAGTCAATGGGCGGTGGAAATGGCCAGGCAGCCAATCAGACTCTCAGTCCTCAGATGTGGAAA TAAAAGCAAAGCACCTGTATGGCTGCCACTCTTTGACCCTTGCTTTCCTCCCTCCTCTTTTTCCCCCAGC CTTCCCCTTTCCTTTTTCCTACCTCTCTCTGTTCGGTTTAGAAACTGATCATAAGTCATATGTTGTTTGG CATTCTGCCTAACCCAGTCTCCCTTCCCAGAGTCATGGACCTCTCTGTTGCTTTATGTTGGTCATGTCCC TGACTGTCCAGATTTCCTCCCACCCCACCCCACCCTCTCCCAGCACCAGCACCTTAGAAACTATTGGCAG AAGAGGGCACATGAGCTGCGGGAGAGTGTATACACTGATCAGTACCTCCCTTGAGGCTGAGCTTCCTCGC AGACCCACAGAAAGGTCTATAAAGGACAAGTGCTTGGCAGAGGGGAAAGTAGAAAGGGTCTTTATTGGCT ATAAGCTGTTTCTAAGTGCTGAGAGTTTTGCTTGAAGAGGGAGCGAGCCATCAAGTTCCCAGCTTTTCAG GAATGAAAGAAGTTCAGCAGTCAGATAATCCTTGTGTCTTGGGTCAGTTTGGCCCTGGCCCTAGAGTGAG GCAAGCCCTTCCTATGAAGATGAGCAAAAATATTATTCCACTTCACTCTGAGTGCTTTCAGGATCTGGGG CTTCAAGACTCACTGCTTCAGTCAGCCTTCATTAGCACCAAAGACTCCATGAAGGTCGTCCCCCACACAA ACATACCCCTTGCGCACCTTCTGCCTACTTTGAGCCGGATCTGGCCTGTGACTAGAAGACCAACCCCTAC GAGGAAATGTGGAGCTCAACGTGTGCACTGCTTGTATGCGTGTGGATGTGTGGATCCCACCTGCCGCCTC CCCATCTGCTCTGGGTGTATTTTTGTTTTTTATAGTTCTAGGTTTACAACAGAGAGGAGTTAATTTATCA ACAGCCTAAAACTGTTGCCAGGTCATCTTACAGTTTAAAACATGCCATCTCATCGGTAGTCCCTGCTCTT TTCCCTCTCCCATCTGCCAGTCTCTCTGTGCCCTCGTGTCTGAGTGCTGTTTGCCCACCTTTTCCAGGTG TAAGAGGCAGAGAGCCTGGACAGCTTTCTCTCTCAGCCATTGTTCACCCCACTTGAAAATTCAAACAAGA AAACTTTGCTTCAAAGATCTCATGTGTGGGAACCACAGCGCCTGGTTGCCTTTCTCCTGTGTATGTGTAA ATTCCTTAATAAATATTGCAGGGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5720177 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5720177 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7601 same embryo
	EMAGE:7604 same embryo
	EMAGE:7606 same embryo
	EMAGE:7603 same embryo
	EMAGE:7602 same embryo
	EurExpress:euxassay_004704 same experiment
	MGI:4828318 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source