EMAGE mouse gene expression spatial database

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EMAGE:7606

Mnt max binding protein ( MGI:109150)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004696. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004696_01	euxassay_004696_02	euxassay_004696_03	euxassay_004696_04

euxassay_004696_05	euxassay_004696_06	euxassay_004696_07	euxassay_004696_08	euxassay_004696_09

euxassay_004696_10	euxassay_004696_11	euxassay_004696_12	euxassay_004696_13	euxassay_004696_14

euxassay_004696_15	euxassay_004696_16	euxassay_004696_17	euxassay_004696_18	euxassay_004696_19

euxassay_004696_20	euxassay_004696_21	euxassay_004696_22	euxassay_004696_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7606_wholemount_strong.wlz

7606_wholemount_moderate.wlz

7606_wholemount_weak.wlz

7606_wholemount_possible.wlz

7606_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7606_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8650
Entity Detected:	Mnt, max binding protein ( MGI:109150)
Sequence:	sense strand is shown >T8650 CCCTTTCTTGTCGGCGGGACTCCGGGGGCCGCGGGGCGGGAGGCATCGGAAGGGAGGAAGAGGAAGAAGG AAGGAAGAAGGCAGTGCCGCCTTTTTTATTTTTTGCATCAAAATTTTTTTAATTTGCAAATTTTTATTTT GCAAATATTTTGGGAGACATTGATTTTTCTCCCCGTGCTCCCCCGTTCTTTTCTGAGGAGTGCGCTGCGC CGCCCAGCCCTGTCGCCCCCCGGAGGTGATCCCTCCCTCCTGCCGGCCCGCCAGCCTGACCTGTGCTTGG CTCGCGGGCCGCAGCCTCGGCCCCGGCGCGCCCCCGGCTGCTCTCGCCGCGATGAGCATAGAGACGCTAC TGGAGGCGGCCCGCTTCCTGGAATGGCAAGCGCAGCAACAACAGAGAGCACGTGAGGAGCAGGAGCGCCT TCGCCTGGAGCGGGAACGTGAGAGAGAGCAGGAGCAGAAGAGGGCAAGTAACTTGGCCAGGTTGGCCCAT GCCCTGCCTGTGGAGGAACCCCGAATCGAGGCTCCACCACTGCCTTTGTCTCCACCGGCACCACCACCCG CACCACCACCACCACTTGCCACCCCTGCTCCACTGACTGTCATTCCTATTCCTGTAGTGACCAATTCCCC GCAGTCTCTACCCCCACCTCCACCTCTACCCCCCGCAGCCCAGCCTCTTCCCCTGGCACCTCGTCAGCCA GCCCTGGTCAGCACCCCTGGACTCAGCATTAAGGAGCCAGTCACCCTGCCCACCAGACCACAGGTGCCCA CCCCTGCTCCCCTCCTGCCTGATGCGAAGACCACAGTTGCGCCCACTGGCAGCCCCAAGCCTTTGCAGCC CCTCCCTGCGCCCATCCTGACCATTGCACCACACCCTGGAGTCCAGCCGCAGCTGGCTCCCCAGCAACCA CCCCCGCCCACGCTTGGGACCCTGAAGTTGGCACCGGCTGAAGAAGCCAAATCCAGTGAACAGAAGAAAA GGCCTGGAGGGATCGGAACCAGAGAAGTCCACAACAAATTGGAGAAGAACAGGAGGGCCCATCTGAAGGA ATGTTTTGAGACCCTGAAGCGCAACATCCCCAACGTGGACGACAAGAAGACTTCGAATCTGAGCGTGCTG CGGACGGCGCTGCGGTACATCCAGTCCCTGAAGAGGAAGGAGAAAGAGTATGAGCATGAGATGGAGCGCC TTGCGCGGGAGAAGATCGCCACCCAGCAGCGGCTGGCAGAGCTCAAGCATGAGCTGAGTCAGTGGATGGA CGTGCTGGAGATAGATCGTGTGCTCCGGCAGACAGGCCAGCCTGAGGACGACCAGGCGTCCACCTCCACA GCCTCCGAGGGCGAGGACAACGTTGACGAGGAGATGGAGGGAGACCGGGCAGGCCTGGGCCCACCTAAGC TGAACCATCGTCCCCAGCCGGAGCTGCTGAAATCCGCCCTGCCAACCCCCAGCACCGCCCCTGCGCCTCT GCCTACCCATCCGCACCCTCACCCCCACCCAGTGGCCCTGTCTCCTGCCCACCTTCCTGTGCAGCAGCAG CAGCCACCACAGCAAAAGACCCCTCTGCCAGCCCCTCCTCCCCCACCTGCCACCCCAACCCAGACGCTAG TGCCAGCTCCGGCCCATCTGGTGGCCACGGCTGGGGGCGGCTCCACGGTCATTGCCCACACAGCCACGAC CCATGCCTCAGTCATCCAGACTGTGAACCATGTTCTGCAGGGGCCGGGGGGCAAGCATATCGCCCACATC GCCCCCTCCGCCCCCAGCCCTGCTGTGCAGCTGGCACCCGCCACACCCCCCATCGGCCACATCACAGTGC ACCCTGCCACCCTCAACCATGTGGCCCACCTTGGCTCCCAGTTGCCCTTGTACCCACAGCCTGTGGCCGT GAGCCAGCCAGTGGCCGTGAGCCACATCGCCCACACCCTGTCGCACCAGCAAGTAAATGGCACAGCCGGG CTGGGGCCCCCAGCCACTGTCATGGCAAAGCCAGCTGTGGGGGCCCAGGTGGTGCACCACCCCCAGCTGG TGGGCCAAACAGTGCTCAACCCTGTGACTATGGTCACCATGCCCTCCTTTCCGGTCAGCACACTCAAGCT GGCTTGAGAATGAGGCCACTTAGGCCCCCAGTGGGGGCAGGGAAGGGGACCTGACTGCCCCTAATCCCAC CCACCAGCTCCACACATTCCAGCCAGGCCCAGGCCCGCCCCACCCACACCACCCCCAGGCCTCCTTAGGG GAAGGGGGTGCAGAGACTCTGAGCCAGGGGAAGGGAGGGGCCCCCAGGGAGTTGGCTGCAGGCAGGAAGT CCTGCTGCAGGAGGACTTGGCAGACAGGAGGATGCTTTTGGTGGACATTCTTGCCTACCCAGCCTGGTGC CAGTTCTGGTGCCGCTCCATCTCCCTGTTTTGTCCCCTGGAAGTCTGCACTGTGGCTTACGTTCTCTGCC TTCCCCTTCCCTGGCCTGCCTTCCTGTGCTGTTGCTGCTATTGCTCTGTCTGGGGAGGTGGCTGAGCTCT CCGGCTTCCCCTCCCCAGCCTCAGAGTCCTGTGGGACCTCTGGATAGGAAACAGGGAGCCCCAGAAAGAT GGGAGATGGGTGGGGAGGGGCCCACCTCTGAGTGCAGGTGACAGATCCTAAGATCTGGAGTCAGGTTAGG ACAAGTGAAGGCCTGGGCCTGGGCCACTCTGACCCACCTGTGGTCACTGTGATTGGCTACAGTAGAAACT GTTCAGAATGAGTCCCAACCAACAATAAACGAGGTCCTGGCTGTTGTCAAGGTTCCCGAGCCTGCCCCTG GGGTGGGGCTTCCCTAGTGCTTCCCCCGGCCCTGGTTTAGGTACTGCCTGCAGCTTGGGAGGTGTGGACC AGCAAGGGCTCTAGGAGCTAAGGTACACTGTGGAGGTATCCCGGGGCTCTTCCCATTCTCCCTCCCTCTC GGTGCCAGTGTTGAGACCCCTCCCTTCCTGGTGGGGCTGCAGTGTCTATGGTGGAAAGGGCCTTTGCTGG GGAAGACAAGGCACAGCATGTGTTTGATGGAGAGCGGGGGTGCTGTGCTTCGGGGGTGCTGTGTCTCCTG CCTCTGCTTCTGCAGACCGCTTTGGCTTCTCTTTTTAAGTGGATATTTATTACAAGTGGCCTTGTGTCAG ACACTCAGTTACTCGCGTGCGCATTCAGGCCCTCACTGGACACTCACCAATGGCCTTTCCGTGTGGGGCA GGAAGCAAGTGCCCCTAAGGTGCGAAGTTTGCACTGGGGGCTTAGGCAGTCCAGGGGCCAGAGGGGAGGA CTCAGGGTCCTTCAGATGACATTGTGAGCAGACACTGAGGCCAGCGAAGGTCCTGAATACATTCTCCCTT GAAATAATGACTCCTCAGGTGCCTCAGAGCTCACAGGTCCTCCTGGCCCTGGAAACCTCCCAGGGCACTT TATTATTATTATTTGTCTCCTGGGGTAGGGAATGGTAGCTAGGACCAGGTCTCAACCCCAGCACAGGAAT CCCAACAAACTGGCTGCCTTCCTGTGGCTGCTGCCAGCCACCTCTACCCACATTCTGACTCTGGGGGCCA CCTCCACTCTCTGCTCTGTGTTGTGACAAGGGGCAGAGTCTGGTGCTGGCTGGGTCAGACCACTTGTCCC ATTAACCCTTCTCTGTGTCTCGAGGCCAGTTGCAGAGTTGACAGCATCGTGTTGGCCCCCAGGCCTGCAC AGATACCTCATCATCCACCCAGCCCTCCCCCCATCCCCCCGGCAGGGGTTCCTCCAGATCTAGTGCCGAA TGACTATGTCCAGATTGGTGGTGATGGGTGTTGTCGCTGTTTTTGTTGTTTGTGACTGCTGTGTGCCCAA GTGGGCAGCCACCTCCCAGCCCTTTCTTGGGCATCTCCCCTTTGAGAGCTGTTAGGACCACATCTGTCCT CACGCTGTGGGACCGCCTGGCCCTTCCCTTCCTATCCCTTCCAGCCTGGGACACACACACACATACACAC ACCTTACCTCTCATGCGTGTTTTACCTGTGCTGTTTGGAGTGGCTCACTGCCTGGGATCTTTACCTCGGG GAGGAAAAGGGGAGAGTGGTTCCCTTGGGGCCAAAGAAGGGCAGGGGATACCTGGAGGGGAGTTGGGTCA CCACAGTCTCTCTCTAGGAACAGCTGCTTCTCCCCCCACCCCAATCCCAGGGTCCCACTCCCAATCCCCA AAGAGGTGGCCCTTGTTTACAGTGAGGACTTGGTCACTGTGTCTCTGTTTCCTGAAATATGAATTGTAGC GACCCCAGACTGTAGAGATTTTTATGTGTTTGGGTACATCTGCTGTGTGGGAAAAAAAAAAAACTACAAA AACCCTAATTTTGTACATATTGTATTTTTACTATTGAACTGTACTCTAGTGGCTGTTCATGCTCTGAGTT TAGGTATTGAGACATGAATACTATCCATGTAATAAGCACTTGCCTGGAATAAAATATAAAACTGAAATAA ATCTGCACTGAAACCTGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5721249 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5721249 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7601 same embryo
	EMAGE:7604 same embryo
	EMAGE:7603 same embryo
	EMAGE:7605 same embryo
	EMAGE:7602 same embryo
	EurExpress:euxassay_004696 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source