EMAGE mouse gene expression spatial database

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EMAGE:7638

Rcan2 regulator of calcineurin 2 ( MGI:1858219)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004716. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004716_01	euxassay_004716_02	euxassay_004716_03	euxassay_004716_04

euxassay_004716_05	euxassay_004716_06	euxassay_004716_07	euxassay_004716_08	euxassay_004716_09

euxassay_004716_10	euxassay_004716_11	euxassay_004716_12	euxassay_004716_13	euxassay_004716_14

euxassay_004716_15	euxassay_004716_16	euxassay_004716_17	euxassay_004716_18	euxassay_004716_19

euxassay_004716_20	euxassay_004716_21	euxassay_004716_22	euxassay_004716_23	euxassay_004716_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7638_wholemount_strong.wlz

7638_wholemount_moderate.wlz

7638_wholemount_weak.wlz

7638_wholemount_possible.wlz

7638_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7638_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8909
Entity Detected:	Rcan2, regulator of calcineurin 2 ( MGI:1858219)
Sequence:	sense strand is shown >T8909 AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCCGGGTCCTTCTTTACTCTGCT GTGCTGCCTCAGAGGCGCTTGCAGCGGGGAGCAGGCTCCAGGTGCCTGAGTCTCCCTAGGCTCAGCTCGC TCTCTTGGCACCCCACCCCCCCAGGAGCAGGGCAGTACAGGACAGTATCCATCCTGAGGAGTATCTTCAC TCCCCACTCTGAGGCTTCCTAACTCTTCACTGAGAATTCTGAAGAGGGGAATGCCAGCCCCTAGCATGGA CTGTGATGTTTCCACTCTGGTCGCCTGTGTGGTGGATGTGGAGGTCTTTACCAATCAGGAGGTTAAGGAA AAATTCGAGGGACTGTTCCGGACCTATGATGAATGTGTGACGTTCCAGCTGTTTAAGAGTTTCCGACGGG TTCGAATAAATTTCAGCCATCCCAAATCTGCAGCCCGTGCCCGGATAGAGCTTCATGAGACTCAGTTCAG AGGGAAGAAGCTAAAACTCTACTTCGCCCAGGTCCAGACCCCAGAGACAGATGGAGACAAACTGCATTTG GCACCTCCACAGCCTGCCAAACAGTTCCTCATCTCACCCCCTTCATCTCCTCCTGTTGGCTGGAAGCCTA TCAGCGATGCCACACCAGTCCTCAACTATGACCTTCTTTATGCTGTGGCCAAACTAGGACCAGGAGAGAA ATATGAGCTGCACGCTGGAACTGAGTCTACACCGAGCGTCGTGGTGCATGTGTGTGACAGCGACATGGAG GAGGAGGAGGACCCAAAGACTTCCCCAAAGCCAAAAATCATCCAGACCCGGCGTCCCGGCCTGCCACCCT CCGTGTCCAACTGAGCACGTCCATGATAACATGCATCTCCTCTTATCATGCTTCCCGCCCCCCCCCTTGT TGTTTGTCCAAACAATTAATTGCCTTTAAGTCCCTGGGTGTTTGGTTGTTTGCGAATCCTTCCTTGTAAT TAAGCCTCTCGGACAAAAGGGCTAGGCAAAGGTGATATGTCTCCCAATCAACTCCTACCCATTAACTACA ATCCATCACTTAGAAAGTTCTAGATAGAGGGTAGTATTTTCTTATTAAATAATCAGTACAGCCCATATCT CTGTTCTTTCATGTTGATCCAAGCCAGAGACATCGGTAATGAAGAGCCTTTATGTTGTTTGTGAGCTGCT CCAGTTCAGTCCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATGTTTTTTTCCTGGCCA CTTATATAGGACCATAAGTAAACTTGGCTTTGACTGCATGAGATCCCCCTATCTGGTCTCACCCAGTCCT CTGCATCCCGACATTCCCAGGACATGCACGATCACCAGCATTGATTTTGATGATTTTGAGGGTATGCCCC AACTTGTGGTATCCCAGCCCTGTCCTGTATAGACAAGGGGCATCCGCAGCACCATTCAGCTCAATAAGTC TGGGAATCATTATTAGTTTGAGTCAATTACATCATATAACTCTGGCCAAATTCCTGGAGGTAAAATTGAG CATGGCCCCAAAGATGTTGAACATCACTACCACAGAGGTCTACCCAGTGTTACTGGGGAGGCTTGTCCAA GTGACAGAGACCGATTTCAGACAAACCTATTTATTATAAAATCTCATAGTGTCTGAATGACTGTCCCCTA CCCCTTTGTATATTTGTACAGATGTTTGAGCTGTGCTTTTAAAATCTGGATGTTTTTTATTTAGTGATTG TTCAACCATTAGCTGCTTAATGACATCATACCTGTGTTGCTTCTGAATGCATTCTGATTCTAATGTAGAT AACCTGATACTATTACATGCTATTGCGGTTCGTGCTAAGTTGTAAAAGGGAACAGCAATGTCTCATGCCA ATAGATAACTGACTAGCCAACCTTTGCTGTCCAAACCACCTGTTTCCAAACAAAGATCCAAGTATCAGTT ATAAGCAACTTGGGTGACGCTAAATCCAAGAAATGTATACAGCTGAGCATTTCAGAGCACAAAGGATGAT GGGTAAACGGATGCCCTTCCCATTCATCCAATGCCCATGCGCTCAAAAGTTCCTTGTTTTTGTTATGTGT GACTTTTGTATATCTCTAGCCCAAAGTGCATTACAGAAGATACACCTGCAGAACCATACTTTCTGCTCCA TGTGTCAAGCCTCATCTATTCCTGTTCATTAACTAGTAACTTTGGATGCAAATACCATTTACCACACAAC AGGCAAGGACTTTCATGGCCTAAGCCTCGGAGTAACACACAAGAACACTAACACAGCCACCTGATGGGGG GATAATTGTGGTTTTTTGATTAAGGTGTATGTTAGTTTCCGCAGAAACTTCAAACCTAGACTGATCATTC AGAAAATTAAAAGTCCGTTCTACTGTGAAATATAGCAATATAGTGCCGGACACGGTACAGTACGATTGAG ACCGAGGACAGTATTGCTTGTGGAATCCTAAGTTTCCATGGGGAAAACAAGTCGTCTTTTCAAAATCAGA TCTGAGGAGTGTACACAGTAAGCAAAGGCTTTGACTTTCCTTTGTTGTGGAAGGTTTTGTTTTCTTTTTT TTAAATTGGCCATGGAAAGACTAGAAATAGTGGCGAGATGATGCCCTCTCGTGGCAGAAACAGTGAAATC GGTTGTATAGATGGATTGATTACAACAGTCAACTACCAGGGGCTGACCTTCTAATCCACATTAACTTTCA GGATGCAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAACAAAATGAAA GTCTGGGAAATAATTTGCGCTTCCAAACAATGATAGCAGAAATGTCTAATTTAGTAAAAGTTGAGTGTGA CTACCTTGCATAGTCCTTAAGAACTCAGTGTTCTAGACGGCCCAGATGATTAGTCTTAGTAAACTGTATT GCCATCTGTTGACGTTAACCTGCTACAGATCAAGGGGTTCTCGCGCTAGATTGAGCATGGGGACGGGGGA GGGGGAGCTTTCCCCAGAAGGAATGGGGAAGCCACGGGACCTCCCAGCATCGAGAAAGTCACCTGGACAC TTCCATGATGCTCATTATGCAAACCTCTTTAGCGCTCTTTTAAGTTTGAAACGTTTTTAAATGGAGGGGA AGGGGAAGGTTTCCACCAACTGAATCATTTGTGCACGTGTACAGCTCAAAGAGCTTAGAGTTCAAATATA TCTGGTGAATGACTGTCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6416901 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6416901 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7640 same embryo
	EMAGE:7642 same embryo
	EMAGE:7639 same embryo
	EMAGE:7637 same embryo
	EMAGE:7641 same embryo
	EurExpress:euxassay_004716 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source