EMAGE mouse gene expression spatial database

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EMAGE:7656

Nkx2-3 NK2 transcription factor related, locus 3 (Drosophila) ( MGI:97348)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005312. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005312_01	euxassay_005312_02	euxassay_005312_03	euxassay_005312_04

euxassay_005312_05	euxassay_005312_06	euxassay_005312_07	euxassay_005312_08	euxassay_005312_09

euxassay_005312_10	euxassay_005312_11	euxassay_005312_12	euxassay_005312_13	euxassay_005312_14

euxassay_005312_15	euxassay_005312_16	euxassay_005312_17	euxassay_005312_18	euxassay_005312_19

euxassay_005312_20	euxassay_005312_21	euxassay_005312_22	euxassay_005312_23	euxassay_005312_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7656_wholemount_strong.wlz

7656_wholemount_moderate.wlz

7656_wholemount_weak.wlz

7656_wholemount_possible.wlz

7656_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7656_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

strong

regional

strong expression: see section 12 13 14 15 16

midgut

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 14 15 16

lower jaw molar

strong

regional

strong expression: see section 08 18 moderate expression: see section 07 weak expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8950
Entity Detected:	Nkx2-3, NK2 transcription factor related, locus 3 (Drosophila) ( MGI:97348)
Sequence:	sense strand is shown >T8950 CAAAACCCAGACCCCCAGGTCCCGGCCAATGGAGGGGATTTAGACTGGACCGCGTCTGTCAAAAGCTCGA CTCCGCACCACCGCGGAGTCCAGAAGAAGAGCTGGAGCCACCGCACTCCCTCCCCGCCCCCGCCGGGATT TATTGAGTATTTGGACTGGACAATTAAGTGGCCCTGATGATGTTACCAAGCCCGGTCACCTCCACCCCTT TCTCAGTCAAAGACATTTTGAATCTGGAGCAGCAGCGGCACTTCCACGGAGCTCACTTGCAGGCGGAATT GGAGCAGCACTTCCACTCGGCACCCTGCATGCTGGCCACGGCTGAAGGGACGCAGTTTTCCGATGCAGGG GAGGAGGACGAGGAAGAAGAGGGAGAGAAACTGTCCTATTTGAACTCACTAGCTGCTGCCGAGGGCCATG GAGATTCGGGTCTCTGTCCTCAGAGCTATGTCCATACAGTTCTCCGAGACGCTTGCAGCGGGCCCAAGGA ACAAGAAGAGGAGGTTGTGAGCGAACGGAGCCAAAAAAGCTGTCAGTTGAAGAAGTCTCTGGAAGCGGCG GGAGACTGTAAGACGAGCGAGGACGGCGAGAGGCCGAAGCCGCGGAGCCGCCGGAAGCCCCGGGTGCTCT TCTCGCAAGCTCAGGTCTTTGAGCTGGAGCGCAGGTTCAAGCAGCAGCGGTACCTGTCGGCGCCCGAGCG CGAGCATCTCGCCAGCAGCCTGAAGCTCACGTCCACGCAGGTGAAAATCTGGTTCCAGAATCGCAGGTAC AAGTGCAAGAGACAGCGGCAGGATAAGTCCCTGGAGCTGGGGACGCACGCGCCGCCGCCGCCACCCCGCC GCGTGGCAGTGCCGGTGCTCGTGCGGGACGGCAAGCCGTGCGTCACGCCCAGCGCACAAACCTACGGCTC GCCCTACGGCGTGGGCGCCGGCGCCTACTCCTACAACAGCTTCCCCGCCTACGGCTACGGGAACTCGGCC GCCGCAGCCGCTGCAGCCGCCGCAGCCGCAGCAGCAGCGGCGGCTTACAGCGGCAGCTACGGCTGCGCCT ATCCGACCGGTGGCGGCGGTGGTGGTGGCGGCACGGCCTCCGCGGCGACCACCGCCATGCAACCCGCCTG CAGCGCCACCGGCGGTGGATCCTTTGTGAACGTGAGCAACCTGGGAGGCTTCGGCAGCGGCGGCGGCGCG CAACCCTTGCATCAAGGTGCTGCAGCCGGGTCCGCGTGCACGCAGGGGACTTTGCAGGGCATCAGGGCTT GGTAAGGCCTGGGCGGGACACGCGGCGGGCGTCCTACTGCACGCTGGGCACCGCGGGAATGAGACACGAG AAAGGACAGACCCAAGGGCCAGGTCCCCTCGTTAAAATAAAAATACAGACGTATCGCTCCTTCCAAGGGG CCATGGTCGCAGCTCACTCCAGGCCTGGGGCATGGAACTCAGCGGAGAAAGGATGCCCTGGTCCAGTCCT CAAGACTGAGACAGAAACACCGGCCAGGCGCCAGGGAAGAGGTGGCCTCCCATCCTGACTCTGCGGAGAG TGCGGGAGGCTGGGACTCCGGCTGTCGCTTGCTCTCCGGAGGAGGAGGAGGGCTTTTCTCTTCCTGCTTT CCCTTGGCCTTCTCCGAGTGTGGTCCGGGAACCGGACCGAACAAGGGGGGAGGGGGGAGAAAGAAAGAAA GAAAGAAAAAGAACAATTTAAAGAAAAAGAAAGGTAGTTGGAGAGAGAACGTGAAGGAGAGGAAAATGGG GTACTAGCTTGAGAAATTCCACCGATCCGCAGCCCCTTTTGCTTGCCCGGAGCGCCACAACGAAGTGGAT TTTTCCTTTTCCAACTGTGTCCCTTCCAAGACAGACTCCGCCCGGATCCCTAGATTGTCAGGGGACTGTT CTTGGAGACCACCACCGCAGTGAGATCGAAAGTTGGGGGAAGGGAGGCAGCTGAGCAAATCCAAGACCTG TCCGCCCAACCTAGGTTGTCGCCAGGTTGGTTTCCTTTCTGTTTTGTATTCTGTATTCCGCACATTATCT CTCTATAACTATATCCACACGCCGTGTACACAGCCGCTGTCACACACTACAGGAGTCAATAAAGAAGGTG CAATATTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6807512 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6807512 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7661 same embryo
	EMAGE:7657 same embryo
	EMAGE:7660 same embryo
	EMAGE:7658 same embryo
	EMAGE:7659 same embryo
	EurExpress:euxassay_005312 same experiment
	MGI:4826723 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source