EMAGE mouse gene expression spatial database

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EMAGE:7657

Ddost dolichyl-di-phosphooligosaccharide-protein glycotransferase ( MGI:1194508)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005304. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005304_01	euxassay_005304_02	euxassay_005304_03	euxassay_005304_04

euxassay_005304_05	euxassay_005304_06	euxassay_005304_07	euxassay_005304_08	euxassay_005304_09

euxassay_005304_10	euxassay_005304_11	euxassay_005304_12	euxassay_005304_13	euxassay_005304_14

euxassay_005304_15	euxassay_005304_16	euxassay_005304_17	euxassay_005304_18	euxassay_005304_19

euxassay_005304_20	euxassay_005304_21	euxassay_005304_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7657_wholemount_strong.wlz

7657_wholemount_moderate.wlz

7657_wholemount_weak.wlz

7657_wholemount_possible.wlz

7657_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7657_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8954
Entity Detected:	Ddost, dolichyl-di-phosphooligosaccharide-protein glycotransferase ( MGI:1194508)
Sequence:	sense strand is shown >T8954 GTCCGTCCCCGCAGCGGCGTGGGCCGCGGGGCCACGGAGTACCCGGACCGCTTCCAAGTGGTACTCCGCC GCTGCGAGCGGCCATTGGGCGGCCGCGAGGGACGCTTCCGGTGCCCAGGTGCTGGCCTGGGCTGCTGGGA GATGAAGATGGATCCCCGCCTCGCCGTCCGCGCCTGGCCCCTCTGCGGGCTGCTGCTGGCCGTGCTCGGC TGCGTCTGCGCCAGCGGCCCCCGCACCCTCGTGCTGCTGGACAACCTGAACGTGCGGGACACGCACTCGC TGTTCTTCCGCAGCCTGAAGGACCGGGGCTTTGAGCTCACCTTCAAGACCGCAGATGACCCCAGCTTGTC CCTCATTAAGTACGGGGAGTTCCTCTATGACAACCTTATCATCTTTTCCCCGTCGGTGGAAGATTTTGGA GGCAACATCAACGTGGAGACCATCAGTGCCTTCATTGATGGTGGCGGCAGCGTTTTGGTGGCTGCCAGCT CTGATATTGGTGACCCTCTTCGGGAGCTGGGCAGTGAGTGTGGGATTGAATTTGATGAAGAGAAAACAGC TGTCATCGACCACCACAACTATGATGTTTCTGACCTTGGCCAGCACACACTCATTGTGGCTGACACTGAG AACCTGCTGAAGGCCCCGACCATTGTTGGCAAGTCATCTCTGAACCCCATTCTCTTCCGAGGAGTTGGAA TGGTGGCAGACCCGGACAATCCCTTGGTTTTGGACATCCTAACAGGCTCTTCAACCTCTTACTCCTTCTT CCCAGATAAACCAATCACCCAGTACCCCCATGCGGTGGGGAGGAACACTCTGCTGATTGCCGGGCTCCAG GCCAGGAACAACGCCCGGGTCATCTTCAGTGGCTCTCTGGATTTCTTCAGCGATGCCTTCTTCAACTCGG CAGTGCAGAAGGCCACACCCGGTGCGCAGAGGTATTCTCAGACAGGCAACTATGAACTAGCTGTGGCCCT CTCACGCTGGGTGTTCAAGGAGGAGGGTGTCCTTCGAGTAGGGCCTGTGTCCCATCACCGGGTGGGCGAG ATGGCTCCACCCAATGCCTACACTGTCACCGACTTGGTGGAGTATAGCATCATCATAGAACAGCTCTCCA ATGGCAAGTGGGTCCCCTTTGATGGTGATGACATTCAGCTGGAGTTCGTGCGCATCGACCCCTTCGTGAG GACCTTCCTGAAGAGGAAAGGTGGCAAGTACAGTGTCCAGTTCAAGCTGCCTGACGTGTATGGTGTATTC CAGTTTAAAGTGGATTACAACCGGCTAGGCTACACCCACCTGTACTCTTCCACCCAGGTGTCAGTGAGGC CACTCCAGCACACGCAGTATGAGCGCTTCATCCCCTCGGCCTATCCCTACTATGCCAGTGCCTTCTCCAT GATGGCCGGGCTCTTCATCTTCAGCATCGTCTTCTTGCACATGAAGGAGAAGGAGAAGTCTGACTGAGCC AGAACCTGGGGTCCACACAGTACGGCCGAGGCCATCACGAATGGATCGGTCGGGTTCTGTTTTGTTTTGT AAAGGCCATGGGAAAATGGCACAGCCTTACCTCAGAGGGCGAGGACTATGGGGTGGGGGGCAGGGAACAT TTTTTTTTTTCTGTAAGTTTTCCAAGTTTGAATCACTCTGTGGCAATTTTTGTATGTGCAGCCGCCCCCA TTTCTAAAATAAAGAGAACCACTGCCCTCACCTGTCACCTGGCCCCATGACACTGATGATGGCAGGGCTG CTGCCTAGGGACACTGTGCCAGAGCCTCCGTGCCTCTCATCTCTCCTCATGGAGGTGGGGATCACACACC CACAGTGTAGTGGAAACTAGACTGACAGCTGGCCTAAGAATTGCTGCTGAAGAGACCCGAGAGGGACTGG AAAGATGACTAGGTTAAGAGCACTGCAGAGGACCCAGGTTCAAGTCCCAGCACCCACATGGCAGCTCACA ACCTTCTATAATGGTAGTCTGAGGAGATCTGATGCCCTTTTGTGGCCTCCCAGGAGACCAGGTATTTAAG TGATACATCGTATGTACAGACAAAACACGATATGCATAAAATAAAGAAAACCCCTGTGTTCCTGTAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6808198 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6808198 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7661 same embryo
	EMAGE:7660 same embryo
	EMAGE:7656 same embryo
	EMAGE:7658 same embryo
	EMAGE:7659 same embryo
	EurExpress:euxassay_005304 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source