EMAGE mouse gene expression spatial database

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EMAGE:7661

Ap2m1 adaptor protein complex AP-2, mu1 ( MGI:1298405)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005298. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005298_01	euxassay_005298_02	euxassay_005298_03	euxassay_005298_04

euxassay_005298_05	euxassay_005298_06	euxassay_005298_07	euxassay_005298_08	euxassay_005298_09

euxassay_005298_10	euxassay_005298_11	euxassay_005298_12	euxassay_005298_13	euxassay_005298_14

euxassay_005298_15	euxassay_005298_16	euxassay_005298_17	euxassay_005298_18	euxassay_005298_19

euxassay_005298_20	euxassay_005298_21	euxassay_005298_22	euxassay_005298_23	euxassay_005298_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7661_wholemount_strong.wlz

7661_wholemount_moderate.wlz

7661_wholemount_weak.wlz

7661_wholemount_possible.wlz

7661_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7661_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8951
Entity Detected:	Ap2m1, adaptor protein complex AP-2, mu1 ( MGI:1298405)
Sequence:	sense strand is shown >T8951 AGCCGAGGCAGCAGACAGGCGAGCTGGGGGCGGGCGGACATCTTGGGATCCGAGAGTGGCCAGGCCGGCC TTGTGGGGGCTGCTGACTCCAGGTCTGTTCTCAGAGCAATGGGTCACAGAGACTGAGCTGCCACCATGAT CGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTTATCTCCCGGGTCTACAGAGATGACATCGGG AGGAATGCTGTGGATGCCTTTCGGGTCAATGTCATTCATGCACGGCAGCAGGTGCGCAGCCCTGTCACAA ACATCGCTCGCACCAGCTTCTTCCATGTTAAGCGGTCCAACATCTGGCTGGCCGCAGTCACCAAGCAGAA TGTCAATGCTGCCATGGTCTTCGAATTCCTCTACAAGATGTGTGATGTAATGGCTGCTTACTTTGGCAAA ATCAGCGAGGAGAACATCAAGAACAATTTTGTGCTCATATACGAGCTGCTGGATGAGATTCTGGACTTTG GCTACCCACAGAACTCAGAGACAGGTGCACTGAAAACCTTCATCACCCAGCAGGGTATCAAGAGTCAGCA TCAGACGAAGGAAGAACAGTCCCAGATCACCAGCCAGGTGACCGGGCAGATTGGCTGGCGGCGAGAAGGC ATCAAGTATCGCCGGAATGAACTCTTCCTAGATGTTCTGGAGAGTGTGAACCTGCTTATGTCCCCACAGG GGCAGGTGCTGAGTGCCCATGTGTCAGGCCGGGTGGTGATGAAGAGTTACCTGAGTGGCATGCCTGAGTG CAAGTTTGGAATGAATGACAAGATTGTCATAGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAAGCAAG AGTGGTAAGCAGTCGATCGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTTTGACT CTGAGCGGAGCATCAGCTTCATCCCTCCCGACGGAGAGTTTGAACTCATGAGATACCGTACTACCAAGGA CATCATCCTTCCTTTCCGGGTGATCCCATTGGTGCGGGAGGTGGGGCGCACCAAACTGGAGGTCAAGGTG GTCATCAAGTCCAACTTCAAGCCCTCACTTCTGGCCCAGAAGATTGAGGTGAGGATCCCGACTCCATTGA ACACAAGCGGGGTACAGGTGATCTGCATGAAGGGGAAGGCCAAGTACAAGGCCAGCGAGAACGCCATTGT ATGGAAGATCAAGCGCATGGCAGGCATGAAGGAATCCCAGATCAGCGCAGAGATTGAGCTCCTGCCCACC AACGATAAGAAGAAATGGGCTCGGCCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCGCCTTCTGGCC TCAAGGTGCGCTACTTGAAGGTATTCGAACCGAAGCTGAACTACAGCGACCATGATGTCATCAAATGGGT GCGATACATTGGCCGCAGTGGCATTTATGAAACCCGCTGCTAGCTGCCTAGTTGCAGCTGGCCCACCTCC CTACCCACCCTTCCCACAGGTCCAAGTACCCCTAGTGACCACTCACCAGTGTCGTTCCTCCGTCCTGCTC TGCTGCCTTTCCTTTCCAATAGCCCCTGAGTATAAGTCTGAACCAGCCACACCACAGTGGAACTGGTCAG GTAGTGCCTGGCTTCCTGGGAGGGTGGTTTTTGAGGCTTCTCTTCCGTCTGTCTGTCCTGGCTTAGCGCC AGGCCCTGAGTTTTGTGACCAAAGCCAGGTGCTCTCCTCTTCTCTCTACCCCTGTGGCCACACCTCTGCA GTGGGAAGGTTGACTACCCCTCACCTCAGAGCTTCCCCAAAGGCCAGTAACAGATCCCCAGTCTTTAGTC CCCACTCTGCTTTGGGATAGTATGAGCTTCGTTTTGTACACGTGTGACTTTGTCCAGTTACAAACCCAAT AAACAATAGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6807694 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6807694 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7657 same embryo
	EMAGE:7660 same embryo
	EMAGE:7656 same embryo
	EMAGE:7658 same embryo
	EMAGE:7659 same embryo
	EurExpress:euxassay_005298 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source