EMAGE mouse gene expression spatial database

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EMAGE:7694

Hdgfrp3 hepatoma-derived growth factor, related protein 3 ( MGI:1352760)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007467. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007467_01	euxassay_007467_02	euxassay_007467_03	euxassay_007467_04

euxassay_007467_05	euxassay_007467_06	euxassay_007467_07	euxassay_007467_08	euxassay_007467_09

euxassay_007467_10	euxassay_007467_11	euxassay_007467_12	euxassay_007467_13	euxassay_007467_14

euxassay_007467_15	euxassay_007467_16	euxassay_007467_17	euxassay_007467_18	euxassay_007467_19

euxassay_007467_20	euxassay_007467_21	euxassay_007467_22	euxassay_007467_23	euxassay_007467_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7694_wholemount_strong.wlz

7694_wholemount_moderate.wlz

7694_wholemount_weak.wlz

7694_wholemount_possible.wlz

7694_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7694_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8976
Entity Detected:	Hdgfrp3, hepatoma-derived growth factor, related protein 3 ( MGI:1352760)
Sequence:	sense strand is shown >T8976 CCGGCGAGCAGGAGAACGACCGGGATGGCGCGTCCGCGGCCCCGCGAGTACAAAGCGGGAGACCTGGTCT TCGCCAAGATGAAGGGCTACCCGCACTGGCCCGCCCGGATTGATGAACTCCCAGAGGGAGCTGTGAAGCC TCCAGCAAACAAGTACCCTATCTTCTTTTTTGGTACCCATGAAACTGCATTTCTAGGTCCTAAAGACCTT TTCCCATATAAGGAATACAAAGACAAGTTTGGAAAGTCAAACAAACGGAAAGGATTTAATGAAGGATTAT GGGAAATTGAAAATAATCCAGGAGTGAAATTTACTGGGTACCAGACAATTCAGCAACAGAGCTCTTCAGA AACTGAGGGAGAAGGTGGAAATACTGCAGATGCAAGCAGTGAGGAAGAAGGTGATAGAGTAGAAGATGGA AAAGGCAAAAGAAAGAATGAAAAAGGAGGCTCAAAACGGAAAAAGTCCTACACGTCAAAGAAGTCTTCTA AACAGTCCCGGAAATCTCCAGGAGACGAAGATGATAAAGACTGCAAAGAAGAGGAGAACAAAAGCAGCTC TGAGGGCGGAGACGCTGGCAATGACACGAGAAACACGACTGCAGACTTGCAGAAAGCCGGTGAAGGGACC TAACTACCGTAATGAATGCTGCATATTGAGAGACACCACAAGAAGGTTAACTGTTTGATTGTCTAATTCT TGGATTTGGATTTGATATGAACCAACGGTCTTTGTTGTCATTGACAGAGCCCCAGTATGTGTGTGCATTA GTCACATTCCTCTCTGTTCTGTTGAAGGAAAAAGAGACATTTTAACCCTTTTAAGGTTGACTTTATTTTA TGTTATTTGGTTGCATGAAGTTGCCCTTAATCACTAAGAATGATGAAGATTTTGCAGACTTCTCTGTGTC TAGGCTCCTTTTCCAAGTCATCAGCTTCCTGCCTCTCTCCCACCATCATCAGATACTCAGCTAAACAGTA ATTCATATTGTTAGGTGACTGCTCAACATAATACTTCAGGATGGGATTTTCTTTGTAGCAGAACCCAAGA ACCAACTCCTAGATACATACTGTTGGTGTATTGGAGATGAAGTTAGAGTCATCTTTCAGTTTTAAGGCCA TGTGTAAAGTTTAACCATATGTACAATACCTATTCTGTATTAGAAATAGCTAGTTGAAACTTAATACTTC TCAAAACTCATGTTCCTGTGTCCACAAACTAAGTTTATATGTGAACTTACTGAGTCTTTTTGTGTCATAC CAAAATAAAGCAAGGACTTATTTTTTTTCCCAAAGCCAATACAATTTGAGCCAATCCCTCAAGATGGTCA CTTTACACTGAAAGCTGAGCTCAGCAGGAGCACTGTGGGAAACAAAGCATTGACTCTTGAGTACAGAGTT TAAAATAGACTTTTCTTTTAGAACTAGAATTAGAGTAGTTAACGTTCATCCGCCAACCATTGTTACGTGT GCATTACTGTCGCTCTTGTGGTGATAGAGTCTTATTTATTTTTATGCCAGCTTATACTGTGAGAACTCAT TAGTCAGTTTGGGTCTCATCAACCCTGCTATGCTTGTCCTTGGAAGATCTTTTGTGTGTGTTTGAGGTTT GTAGCTGAGAAGTTTACTGTAAAAAAAAAAAATCAAAAACAAAAAAATGTATTGTTTTTACAGAATAAAT TTATTGGAATGTGTACTGGGAGTAAGGTTTGAGGTTATAAACAGCCGAGTTAGTGTTATTTGGCTTCATA TGTGTAATGTGAAGTATTAATGTGTTTCTTGTATTATAGCAAAGATTGACAGCAAATTGACATTAGAATT AAGTGCAGGTGAAAATTTTATTTTTTCTATCATGTTTCTTTTTAAGTATTCTGGATGTTAGGTTAAGTGA AAGCCGTGGGGGGACAGGAGGGAGCAACAGCAGGAGCTGCAGCAGCAGGGGCTGTCTGCACGTCTGCGAC AGCCCAGCTCAGGGGACCCTGCATTTACACACAGCCTTCCGTGCATCCAGTTTGCTTTTGGTGTTCTTAC TGTGTTGAAGAACTAATGAACGTGAAGAGAAAATAGAAGAAGACCTAAGAGCCCAGTGTCGTTGTGAAGA TGGAGGTGATGTGGGGCTCTGGATGCACTGGTCTACTCACCCCTCCCTCCAGGACATCACTGTCCTTTGG GCTTGCTCCTGCCGGTTAGCAATCAGTGGTCCCCTGTTGTGCTCACGCAGTTCCATGTATGGAAAATTCA TTAAGAGACAGGCTGTTAAGTCAGGGTTTTTTCTTTAAAAGGTACTTTTTAATTTTAAATATTTTAGTTT AAAATAATGAAATTTGACAAAAGATCTCTTCATTATCATGCAAAACATATGGAGAATGTAGATAGCATTT TTAAATTGGAACATATAACAAAGGTTTTTTTGTTTGTTTGTTTGTTTGAGGCAGGGTTTCTCTGTGTAGC CCTGATTATCCTGGAACTCACTCTGTAGACCAGGCTAGCCTGGAACTCAAAAGATCAGCCTGTCTCTGAA TCCCAAGTGCAAGGATTAAAGGCGTGTGCTACCACTACCTGGCACAGATTTCTTAAATAAAAAGTTCAAA ATTTGTCATGAAAATGTATATATAAAATGCTTCAAAATTAATAAAGTTTATCTTCATAAACACCAAAAAT AATTTTATTGAAGGTATACACAGTATTGAAGGTATACACAGAAACACAAGAGAAATATTATCAACCAATA AAACAAGTGTTCATTAAGGCAACTAATAAAGTTGATTTGTGTCATCTTAGCTATTCAAATTCACAATAGA AGTTTTGACAGCAATTTTTAAAATGGCAATAAATAATATGCTTTCCTTGTGAAAACACTTGAATCAATTT AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810355 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810355 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7696 same embryo
	EMAGE:7695 same embryo
	EMAGE:7699 same embryo
	EMAGE:7697 same embryo
	EMAGE:7698 same embryo
	EurExpress:euxassay_007467 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source