EMAGE mouse gene expression spatial database

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EMAGE:7695

Fam171a2 family with sequence similarity 171, member A2 ( MGI:2448496)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007459. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007459_01	euxassay_007459_02	euxassay_007459_03	euxassay_007459_04

euxassay_007459_05	euxassay_007459_06	euxassay_007459_07	euxassay_007459_08	euxassay_007459_09

euxassay_007459_10	euxassay_007459_11	euxassay_007459_12	euxassay_007459_13	euxassay_007459_14

euxassay_007459_15	euxassay_007459_16	euxassay_007459_17	euxassay_007459_18	euxassay_007459_19

euxassay_007459_20	euxassay_007459_21	euxassay_007459_22	euxassay_007459_23	euxassay_007459_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7695_wholemount_strong.wlz

7695_wholemount_moderate.wlz

7695_wholemount_weak.wlz

7695_wholemount_possible.wlz

7695_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7695_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8977
Entity Detected:	Fam171a2, family with sequence similarity 171, member A2 ( MGI:2448496)
Sequence:	sense strand is shown >T8977 GGCGCCGCAGCCGGAGCGGGAGCCCAACCCGGAGCTGCACAGAGCGGTGCAGAACTGGGGCTGAGCGGGG CCGGCCGATCAGGGCCGGGCGGCAACGATGGACCGCTAGGGCCCGGCCGAGCCCCGCGATGCCGCCGCCG AGTGGCCCCGGCGTCCTCGCGCGGCTGCTGCCGCTGCTGGGGCTGCTGCTCGGCGGCGCCTCCCGGGCTC CCGGCAAGTCTCCGCCGGAGCCCCCCAGCCCGCAGGAGTGTCTTGGGAAGGGACCCTGGATTGGCCTCCA AACAGAGGGAGGTGGGAGGGACCCGGTCCCCCATGGGAGGGGCTGGGGAAGAGGGCTGGGGTGGGGGAGC AGTGCCTGTATAAACGCCTGCCTGTCAGTTCCACCTACAGCCAGCTTTGGGCTTCGCTCACACCGGCCAG CACCCAGCAGGAAATGCGGGCTTTCCCTGCCTTCCTGGGGACCGAGGCCTCCAGCTCAGGCAATGGCTCC TGGCTGGAGCTGATACCACTGGCTGCTGTGAGCGTCCACCTGCTAACAGGCAATGGGACAGAGGTGCCTC TCTCAGGCCCCATTCACCTGTCCCTGCCAGTGCCCTCAGAGCCTCGTGCCCTTGCTGTGGGCACCAGCAT TCCAGCCTGGAGGTTTGACCCCAAGAGTGGACTGTGGGTACGAAATGGCACTGGTGTGATTCGGAAGGAA GGCCGACAGCTCTACTGGACTTTTGTCTCCCCGCAGCTAGGGTACTGGGTAGCTGCCATGGCCTCCCCTA CATCTGGGCTGGTTACCATCACCTCAGGCATTCAGGACATTGGCACCTACCACACAATCTTCCTGCTGAC GATCCTGGCTGCCCTGGCGCTGCTGGTGCTCATTCTGCTGTGTCTACTGATCTACTACTGCAGAAGACGC TGCTTGAAGCCTAGACAACAGCACCGCAAGCTGCAGCTCTCCGGCCCCTCGGACAACAAACGAGATCAGG CTACGTCGATGTCTCAACTCCATCTCATCTGCGGGGGACCCCTGGAGCCTACGTCGTCGGGAGACCCCGA GGCCCCTCCCCCAGGCTCCCTACACTCGGCCTTCTCCAGCTCCCGAGACTTAGCATCCTCGCGGGATGAC TTCTTCCGTGCCAAACCGCGCTCCGCCAGCCGACCGGCCGCCGAGCCTCCTGGTGCCCGCACCGTTGAGG GTGCCGGGCTCAAGAGCGCCCGCTCCGTCGAGGGTCCTGGCGGGCTGGAGCCCAGCCTGGACGAATACCG GCGGGGTCCAGCCGGGGCCGCGGCCTTCCTGCATGAGCCTCCCTCGCCGCCGCCATCCTTCGACCATTAT CTGGGCCACAAGGGGGCGGCCGAGAGCAAGACCCCCGACTTTCTGCTGTCACAGTCGGTAGACCAGCTGG CGCGGCCGCCGTCCCTCAGCCAACCGGGCCAGCTCATTTTCTGCGGTTCCATCGACCACCTCAAGGACAA CGTGTACCGCAATGTCATGCCCACCCTGGTGATCCCCGCACACTATGTGCGCCTGGGCGGAGAGGCGGGC GCCGCGGGAGTGGGCGACGAAGCCACCCCGCCGGAGGGCTCGGCCGCCGGCCCCGCGCGCCCCTTCCCTC AACCCGACCCCCAGCGCCCCCTGATGCAGGGCCACGCGGGTGCGGGAGGCGACAGCGGTGGCGGCGAGGG CTGGGGCGGCGGGCGCTCCGCACCGGTCAGCGGCTCGGTCACCATTCCGGTGCTGTTCAACGAGTCCACC ATGGCGCAGCTCAACGGCGAGCTGCAGGCTCTAACCGAGAAGAAGCTGCTGGAACTGGGAGTGAAGCCTC ACCCGCGCGCCTGGTTCGTATCCCTGGACGGACGTTCCAATTCACAAGTGCGCCACTCCTACATCGACCT GCAGGCGGGCAGCGGCGGCCGCAGCACCGATGCCAGCCTGGACTCAGGCGTCGACGTGCACGAGGCTCGA CCTGCGCGCCGCCGGCTCCCGAGGGAAGAGCGGGAGCGCGCGCAGCTGCCCGCGCCGCCGCCGCCCGCGC CCCCGCGCCTGGCGCTCAGCGAAGACACAGAGCCCAGCAGCAGCGAGAGCCGCACCGGCCTCTGCTCGCC GGAGGACAACTCGCTAACGCCCCTGCTGGACGAGGTGGTGGCACCCGAAGGCCGGGCAGCCACGGTACCC AGGGGTCGGGGCCGCAGCCGTGGGGACAGTTCCCGCAGCAGCGCCAGTGAGCTGCGGCGCGATTCGCTCA CCAGCCCGGAGGACGAGCTGGGGGCGGAGGTGGGCGACGAGGCTGGCGACAAGAAGAGCCCGTGGCAGCG GCGGGAGGAGCGGCCGCTAATGGTGTTCAACGTCAAGTAGTGCGGCCCGGGGGTCACCCACCGCCCCTGC AGGTTCCCAGAGTGCGCGCCCCGGGATGCTATGGCGGAGGGGCATGCGCTCTTATCCCGGAGAAGAGCTC AGGGGTCGGCAGAGCTCATCTGTGGTATGTGCTGGGGGAGTGTCTTGGGAAGGGACCCTGGATTGGCCTC CAAACAGAGGGAGGTGGGAGGGGCAATCTCTGTGGACCCTTGGGAGGGGAGGGGTGCTGCTTGGATGTCA CAGGGCTGGTGGCTGGGGCGATCCTGGAGACTAGAGGATAATGTGATAACACTCTTGACAAAAAAAAAAT ACAAAGCTGGTGAGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810415 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810415 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7696 same embryo
	EMAGE:7699 same embryo
	EMAGE:7697 same embryo
	EMAGE:7694 same embryo
	EMAGE:7698 same embryo
	EurExpress:euxassay_007459 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source